Genomic and virulence insights of Western European Aeromonas salmonicida subsp. salmonicida and development of Galleria mellonella infection assay. - 2025
Galleria mellonella; European Aeromonas salmonicida; antimicrobial resistance; mobile genetic elements; virulence; Anti-Bacterial Agents; Type III Secretion Systems; Animals; Larva/microbiology; Virulence/genetics; Anti-Bacterial Agents/pharmacology; Genome, Bacterial; Furunculosis/microbiology; Drug Resistance, Multiple, Bacterial/genetics; Plasmids/genetics; Microbial Sensitivity Tests; Genomics; Type III Secretion Systems/genetics; Aeromonas salmonicida/genetics; Aeromonas salmonicida/pathogenicity; Aeromonas salmonicida/drug effects; Moths/microbiology; Gram-Negative Bacterial Infections/microbiology; Gram-Negative Bacterial Infections/veterinary; Aeromonas salmonicida; Antimicrobial resistances; European aeromonas salmonicidum; Genetic elements; Infection models; Mobile genetic element; Salmonids; Drug Resistance, Multiple, Bacterial; Furunculosis; Gram-Negative Bacterial Infections; Larva; Moths; Plasmids; Biotechnology; Applied Microbiology and Biotechnology
Abstract :
[en] [en] AIMS: Aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis, a fish disease highly aggressive for salmonids and responsible for significant economic losses in aquaculture worldwide. This study aimed to explore genomic and antimicrobial resistance traits of Western European A. salmonicida subsp. salmonicida strains and to develop an adapted infection model using larvae of the greater wax moth Galleria mellonella to assess the pathogenic potential of this psychrophilic subspecies.
METHODS AND RESULTS: Three A. salmonicida subsp. salmonicida strains, isolated from salmonids displaying clinical signs of furunculosis, were tested against a panel of antibiotics and sequenced to characterize their genome. Virulence was evaluated in G. mellonella larvae using bacterial doses ranging from 101 to 106 CFU/larva. Two isolates exhibited multidrug resistance to antibiotics commonly used against furunculosis. Although closely related to the reference strain A449, genomic analyses revealed multiple plasmids known to encode antibiotic resistance genes. Virulence assays showed that this subspecies was lethal at doses as low as 101 CFU/larva, and that a fully functional Type III secretion system (T3SS) is not essential for the infection of G. mellonella, likely due to the presence of other virulence factors in T3SS-deficient strains.
CONCLUSIONS: These findings enhance the genomic characterization of European A. salmonicida subsp. salmonicida and validate the use of G. mellonella larvae as a relevant alternative infection model for studying this psychrophilic subspecies.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Desmecht, Salomé ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)
Touzain, Fabrice; Viral Genetics and Biosafety Unit, Ploufragan-Plouzané-Niort Laboratory, ANSES, Rue des Fusillés, Ploufragan, 22440, France
Olivier, Thibaut ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Antoine, Céline ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Beven, Véronique; Viral Genetics and Biosafety Unit, Ploufragan-Plouzané-Niort Laboratory, ANSES, Rue des Fusillés, Ploufragan, 22440, France
Meex, Cécile; Department of Clinical Microbiology, University Hospital of Liège, Avenue de l'Hôpital, Liège, 4000, Belgium
Lieffrig, François; Fish Pathology Laboratory, CER groupe, Rue de la Science, Marche-en-Famenne, 6900, Belgium
Charette, Steve J ; Institut de Biologie Intégrative et des Systèmes (IBIS), Laval University, Avenue de la Médecine, Quebec City, G1V 0A6, Canada
Thiry, Damien ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie vétérinaire et maladies bactériennes animales
Language :
English
Title :
Genomic and virulence insights of Western European Aeromonas salmonicida subsp. salmonicida and development of Galleria mellonella infection assay.
This research was funded by the University of Li\u00E8ge, Belgium, \u201CCr\u00E9dits sectoriels de la Recherche en Sciences de la Sant\u00E9\u201D 2022.
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