Incorporating quercetin nanocrystals in chitosan-polyvinyl alcohol composite film with cinnamon essential oil loaded Pickering emulsions for enhanced controlled release properties
Fan, Simin; Zhang, Dequan; Yang, Qingfenget al.
2025 • In Food Packaging and Shelf Life, 52, p. 101678
[en] Despite extensive efforts in developing bio-based active packaging, most current films still suffer from poor release kinetics of active ingredients. This study presents an innovative strategy to improve the controlled-release performance of antimicrobial packaging by incorporating cinnamon essential oil (CEO)-loaded Pickering emulsions as carriers and quercetin nanocrystals (QNs) as nanofillers into a chitosan-polyvinyl alcohol composite (CS-PVA/PEs/QNs). The resulting CS-PVA/PEs/QNs film exhibited excellent mechanical properties, achieving a tensile strength of 39.09 ± 0.46 MPa. We attribute this enhancement to a strengthened hydrogen-bonding network among QNs, the CEO-loaded Pickering emulsions, and the CS-PVA matrix, as confirmed by FTIR, XRD, and TGA. Furthermore, the film demonstrated remarkable antimicrobial activity (>99.99 % inhibition against food spoilage bacteria) and a significantly enhanced antioxidant capacity (approximately five-fold increase). The incorporation of QNs effectively modulated the CEO release kinetics, which followed a Fickian diffusion mechanism in high-fat food simulants, thereby suggesting a potential for long-term antimicrobial efficacy. Owing to these controlled-release antimicrobial characteristics, the CS-PVA/PEs/QNs film proved highly effective in preserving fresh meat, extending its shelf life to 11 days at 4 • C. This work provides a promising strategy for designing advanced controlled-release systems and contributes to the development of highperformance antimicrobial packaging.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Fan, Simin ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Zhang, Dequan; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Yang, Qingfeng; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Zhu, Chaoqiao; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Richel, Aurore ; Université de Liège - ULiège > TERRA Research Centre > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Li, Cheng; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Tian, Ming; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Li, Xin; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Hou, Chengli ; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Incorporating quercetin nanocrystals in chitosan-polyvinyl alcohol composite film with cinnamon essential oil loaded Pickering emulsions for enhanced controlled release properties
Almasi, H., Azizi, S., Amjadi, S., Development and characterization of pectin films activated by nanoemulsion and Pickering emulsion stabilized marjoram (Origanum majorana L.) essential oil. Food Hydrocolloids, 99, 2020, 105338, 10.1016/j.foodhyd.2019.105338.
Chang, H., Xu, J., Macqueen, L.A., Aytac, Z., Peters, M.M., Zimmerman, J.F., Xu, T., Demokritou, P., Parker, K.K., High-throughput coating with biodegradable antimicrobial pullulan fibres extends shelf life and reduces weight loss in an avocado model. Nature Food 3:6 (2022), 428–436, 10.1038/s43016-022-00523-w.
Chen, X., Lan, W., Xie, J., Characterization of active films based on chitosan/polyvinyl alcohol integrated with ginger essential oil-loaded bacterial cellulose and application in sea bass (Lateolabrax japonicas) packaging. Food Chemistry, 441, 2024, 138343, 10.1016/j.foodchem.2023.138343.
Cheng, Y., Cai, X., Zhang, X., Zhao, Y., Song, R., Xu, Y., Gao, H., Applications in Pickering emulsions of enhancing preservation properties: Current trends and future prospects in active food packaging coatings and films. Trends in Food Science Technology, 151, 2024, 10.1016/j.tifs.2024.104643.
Du, H., Sun, X., Chong, X., Yang, M., Zhu, Z., Wen, Y., A review on smart active packaging systems for food preservation: Applications and future trends. Trends in Food Science Technology, 141, 2023, 104200, 10.1016/j.tifs.2023.104200.
Fan, S., Wang, D., Wen, X., Li, X., Fang, F., Richel, A., Xiao, N., Fauconnier, M.-L., Hou, C., Zhang, D., Incorporation of cinnamon essential oil-loaded Pickering emulsion for improving antimicrobial properties and control release of chitosan/gelatin films. Food Hydrocolloids, 138, 2023, 108438, 10.1016/j.foodhyd.2022.108438.
Fan, S., Yang, Q., Wang, D., Zhu, C., Wen, X., Li, X., Richel, A., Fauconnier, M.-L., Yang, W., Hou, C., Zhang, D., Zein and tannic acid hybrid particles improving physical stability, controlled release properties, and antimicrobial activity of cinnamon essential oil loaded Pickering emulsions. Food Chemistry, 446, 2024, 138512, 10.1016/j.foodchem.2024.138512.
Fan, S., Yang, Q., Zhu, C., Li, X., Richel, A., Fauconnier, M.-L., Fang, F., Zhang, D., Hou, C., Zein/chitosan Janus film incorporated with tannic acid and cinnamon essential oil co-loaded Pickering emulsion for sustained controlled release and pork preservation. International Journal of Biological Macromolecules, 2024, 138429, 10.1016/j.ijbiomac.2024.138429.
Guo, X., Luo, W., Wu, L., Zhang, L., Chen, Y., Li, T., Li, H., Zhang, W., Liu, Y., Zheng, J., Wang, Y., Natural products from herbal medicine self-assemble into advanced bioactive materials. Advanced Science, 11(35), 2024, 2403388, 10.1002/advs.202403388.
Ji, C., Wang, Y., Recent advances in Pickering emulsions for inhibiting foodborne bacteria. Current Opinion in Food Science, 61, 2025, 101265, 10.1016/j.cofs.2024.101265.
Jiang, H., Zhao, S., Li, Z., Chen, L., Mo, H., Liu, X., Swan-feathers inspired smart-responsive sustainable carboxymethyl cellulose/polyvinyl alcohol based food packaging film for robustly integrated Intelligent and Active Packaging. Nano Today, 56, 2024, 102272, 10.1016/j.nantod.2024.102272.
Li, F., Zhe, T., Ma, K., Zhang, Y., Li, R., Cao, Y., Li, M., Wang, L., One stone two birds: Multifunctional flavonol nanocrystals enable food packaging to both preserve freshness and visually monitor freshness. Chemical Engineering Journal, 453, 2023, 139760, 10.1016/j.cej.2022.139760.
Lucas-González, R., Yilmaz, B., Mousavi Khaneghah, A., Hano, C., Shariati, M.A., Bangar, S.P., Goksen, G., Dhama, K., Lorenzo, J.M., Cinnamon: An antimicrobial ingredient for active packaging. Food Packaging and Shelf Life, 35, 2023, 101026, 10.1016/j.fpsl.2023.101026.
Ma, Z., Xing, Z., Zhao, Y., Zhang, H., Xin, Q., Liu, J., Qin, M., Ding, C., Li, J., Lotus leaf inspired sustainable and multifunctional Janus film for food packaging. Chemical Engineering Journal, 457, 2023, 141279, 10.1016/j.cej.2023.141279.
Mather, A.E., Gilmour, M.W., Reid, S.W.J., French, N.P., Foodborne bacterial pathogens: Genome-based approaches for enduring and emerging threats in a complex and changing world. Nature Reviews Microbiology 22:9 (2024), 543–555, 10.1038/s41579-024-01051-z.
Miao, W., Gu, R., Shi, X., Zhang, J., Yu, L., Xiao, H., Li, C., Indicative bacterial cellulose films incorporated with curcumin-embedded Pickering emulsions: Preparation, antibacterial performance, and mechanism. Chemical Engineering Journal, 495, 2024, 153284, 10.1016/j.cej.2024.153284.
Parfitt, J., Croker, T., Brockhaus, A., Global food loss and waste in primary production: A reassessment of its scale and significance. Sustainability, 13(21), 2021, 12087, 10.3390/su132112087.
Qin, W., Zou, L., Hou, Y., Wu, Z., Loy, D.A., Lin, D., Characterization of novel anthocyanins film @ carbon quantum dot nanofiber intelligent active double-layer film, physicochemical properties and fresh-keeping monitoring in Ictalurus punctatus fish. Chemical Engineering Journal, 496, 2024, 154041, 10.1016/j.cej.2024.154041.
Rojas, A., Misic, D., Zizovic, I., Dicastillo, C.L. d, Velásquez, E., Rajewska, A., Rozas, B., Catalán, L., Vidal, C.P., Guarda, A., Galotto, M.J., Supercritical fluid and cocrystallization technologies for designing antimicrobial food packaging PLA nanocomposite foams loaded with eugenol cocrystals with prolonged release. Chemical Engineering Journal, 481, 2024, 148407, 10.1016/j.cej.2023.148407.
Wang, D., Fan, S., Li, X., Chen, L., Wen, X., Xu, Y., Zhu, C., Hou, C., Zhang, D., Carboxymethyl chitosan/polyvinyl alcohol hydrogel films by incorporating MSNs as ε-PL carrier with pH-responsive controlled release and antibacterial properties. Food Packaging and Shelf Life, 46, 2024, 101360, 10.1016/j.fpsl.2024.101360.
Wang, L., Liu, T., Liu, L., Liu, Y., Wu, X., Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat. Meat Science, 185, 2022, 108706, 10.1016/j.meatsci.2021.108706.
Wen, X., Zhang, D., Li, X., Ding, T., Liang, C., Zheng, X., Yang, W., Hou, C., Dynamic changes of bacteria and screening of potential spoilage markers of lamb in aerobic and vacuum packaging. Food Microbiology, 104, 2022, 103996, 10.1016/j.fm.2022.103996.
Xia, J., Sun, X., Jia, P., Li, L., Xu, K., Cao, Y., Lü, X., Wang, L., Multifunctional sustainable films of bacterial cellulose nanocrystal-based, three-phase pickering nanoemulsions: A promising active food packaging for cheese. Chemical Engineering Journal, 466, 2023, 143295, 10.1016/j.cej.2023.143295.
Xu, W., Jia, Y., Li, J., Sun, H., Cai, L., Wu, G., Kang, M., Zang, J., Luo, D., Pickering emulsion with high freeze-thaw stability stabilized by xanthan gum/lysozyme nanoparticles and konjac glucomannan. International Journal of Biological Macromolecules, 261, 2024, 129740, 10.1016/j.ijbiomac.2024.129740.
Yu, Y., Gong, M., Wang, S., Wang, X., Liu, Y., Huang, D., Guan, H., Liu, H., Chen, Y., Jiang, Y., Li, D., Pectin-based cinnamon essential oil Pickering emulsion film with two-sided differential wettability: A major role in the spatial distribution of microdroplets. International Journal of Biological Macromolecules, 277, 2024, 133727, 10.1016/j.ijbiomac.2024.133727.
Zhang, W., Ezati, P., Khan, A., Assadpour, E., Rhim, J.-W., Jafari, S.M., Encapsulation and delivery systems of cinnamon essential oil for food preservation applications. Advances in Colloid and Interface Science, 318, 2023, 102965, 10.1016/j.cis.2023.102965.
Zhang, Y., Jiang, W., Effective strategies to enhance ultraviolet barrier ability in biodegradable polymer-based films/coatings for fruit and vegetable packaging. Trends in Food Science Technology, 139, 2023, 104139, 10.1016/j.tifs.2023.104139.
Zhao, Y., Meng, Z., Shao, L., Dai, R., Li, X., Jia, F., Employment of cold atmospheric plasma in chilled chicken breasts and the analysis of microbial diversity after the shelf-life storage. Food Research International, 162, 2022, 111934, 10.1016/j.foodres.2022.111934.
Zhu, C., Yang, Q., Tian, M., Yang, W., Min, C., Fan, S., Wang, D., Li, X., Zhang, D., Hou, C., Sustainable nanofiber films based on polylactic acid/modified cellulose nanocrystals containing various types of polyphenols, exhibiting antioxidant activity and high stability. Food Chemistry, 477, 2025, 143514, 10.1016/j.foodchem.2025.143514.
