[en] This study aimed to develop novel functional cheeses from camel and bovine milk, fortified with varying concentrations of Spirulina (Arthrospira platensis) powder at 0.1, 0.25, 0.5, and 1.0 %, and to assess their physicochemical, textural, and bioactive properties. The antioxidant assays demonstrated that cheeses from camel and cow's milk revealed significant antioxidant activities, which were enhanced with the addition of Spirulina (p < 0.0001). Increasing Spirulina content led to a decrease in L* and a* values, along with an increase in b* values compared to the control samples (p < 0.0001). The textural properties of both camel and bovine cheeses were significantly affected, with a marked reduction in hardness as Spirulina concentration increased (p < 0.0001). However, the impact on texture was minimal in camel milk cheese. The composition of camel milk resulted in a softer curd structure, allowing for the even distribution of Spirulina without disrupting the protein network. In contrast, Spirulina interacted with the denser protein matrix of bovine cheese, leading to a notable decline in textural properties. Overall, the results suggest that Spirulina may serve as a natural component in camel cheeses formulations in order to enhance their nutritional profile while maintaining desirable functional properties.
Disciplines :
Food science
Author, co-author :
Lajnaf, Roua; Alimentary Analysis Unit, National Engineering School of Sfax, Sfax, Tunisia ; Montpellier University, Montpellier Cedex 5, France
Attia, Hamadi; Alimentary Analysis Unit, National Engineering School of Sfax, Sfax, Tunisia
Ayadi, Mohamed ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Language :
English
Title :
Development and characterization of spirulina (Arthrospira platensis)-fortified cheeses from camel and bovine milk
This research was supported by the Ministry of Scientific Research and Technology of Tunisia . We would like to sincerely thank Dr. Fatma Chakroun for her valuable assistance with the statistical analyses of this work.
Lucas, B.F., de Morais, M.G., Santos, T.D., Costa, J.A.V., Effect of Spirulina addition on the physicochemical and structural properties of extruded snacks. Food Sci. Technol. 37 (2017), 16–23.
Terpou, A., Bosnea, L., Mataragkas, M., Markou, G., Influence of incorporated arthrospira (Spirulina) platensis on the growth of microflora and physicochemical properties of feta-type cheese as functional food. Proceedings, 2020, MDPI, 97.
Karimy, M.F., Febrisiantosa, A., Sefrienda, A.R., Setiyawan, A.I., Pratiwi, D., Khasanah, Y., Wahyuningsih, R., Suwito, W., Kurniawan, T., Hariyadi, S., The impact of adding different levels of spirulina on the characteristics of halloumi cheese. Int. J. Gastron. Food Sci., 38, 2024, 101050.
Garbowska, M., Berthold-Pluta, A., Stasiak-Różańska, L., Kalisz, S., Pluta, A., The impact of white mulberry, green barley, chia seeds, and spirulina on physicochemical characteristics, texture, and sensory quality of processed cheeses. Foods, 12, 2023, 2862.
Bosnea, L., Terpou, A., Pappa, E., Kondyli, E., Mataragas, M., Markou, G., Katsaros, G., Incorporation of Spirulina platensis on traditional greek soft cheese with respect to its nutritional and sensory perspectives. Proceedings, 2020, MDPI, 99.
Voșgan, Z., Dumuta, A., Marian, M., Mihalescu, L., Pop, F., The effect of incorporating spirulina in mozzarella cheese as a functional food. J. Agroaliment. Process. Technol., 27, 2021.
Al haj, O.A., Al Kanhal, H.A., Compositional, technological and nutritional aspects of dromedary camel milk. Int. Dairy J. 20 (2010), 811–821.
Izadi, A., Khedmat, L., Mojtahedi, S.Y., Nutritional and therapeutic perspectives of camel milk and its protein hydrolysates: a review on versatile biofunctional properties. J. Funct. Foods, 60, 2019, 103441.
Badawy, A.A., El-Magd, M.A., AlSadrah, S.A., Therapeutic effect of camel milk and its exosomes on MCF7 cells in vitro and in vivo. Integr. Cancer Ther. 17 (2018), 1235–1246.
FAOSTAT, Food and Agriculture Organization of the United Nations, Crops and livestock products. https://www.fao.org/faostat/en/#data/QC, 2023. (Accessed 22 April 2025)
Muñoz-Bas, C., Muñoz-Tebar, N., Viuda-Martos, M., Sayas-Barberá, E., Pérez-Alvarez, J.A., Fernández-López, J., Application of date-coproducts for the fortification of fresh goat cheese: effect on their nutritional, technological, physicochemical, microstructural, microbiological and sensory properties. Appl. Food Res., 4, 2024, 100619.
Garofalo, G., Buzzanca, C., Ponte, M., Barbera, M., D'Amico, A., Greco, C., Mammano, M.M., Franciosi, E., Piazzese, D., Guarrasi, V., Comprehensive analysis of Moringa oleifera leaves’ antioxidant properties in ovine cheese. Food Biosci., 61, 2024, 104974.
Shoukat, M., Hervé, V., Sarthou, A.-S., Peron, A.-C., Danel, A., Swennen, D., Bonnarme, P., Dugat-Bony, E., Iron fortification modifies the microbial community structure and metabolome of a model surface-ripened cheese. Int. J. Food Microbiol., 427, 2025, 110971.
Garofalo, G., Ponte, M., Greco, C., Barbera, M., Mammano, M.M., Fascella, G., Greco, G., Salsi, G., Orlando, S., Alfonzo, A., Improvement of fresh ovine “Tuma” cheese quality characteristics by application of oregano essential oils. Antioxidants, 12, 2023, 1293.
El-Zeini, H.M., Microstructure, rheological and geometrical properties of fat globules of milk from different animal species. Polish J. Food Nutr. Sci., 15, 2006, 147.
El Hatmi, H., Jrad, Z., Mkadem, W., Chahbani, A., Oussaief, O., Ben Zid, M., Zaidi, S., Khorchani, S., Belguith, K., Mihoubi, N.B., Fortification of soft cheese made from ultrafiltered dromedary milk with Allium roseum powder: effects on textural, radical scavenging, phenolic profile and sensory characteristics. LWT, 132, 2020, 109885.
Lajnaf, R., Attia, H., Ayadi, M.A., A review of camel β-casein: from purification processes, to bioactivity and techno-functionality. Food Biosci., 62, 2024, 105060.
Baig, D., Sabikhi, L., Khetra, Y., Shelke, P.A., Technological challenges in production of camel milk cheese and ways to overcome them–a review. Int. Dairy J., 129, 2022, 105344.
Yüksel, Z., Erdem, Y.K., Detection of the milk proteins by RP-HPLC. GIDA-J. Food. 35 (2010), 5–11.
Lajnaf, R., Zouari, A., Trigui, I., Attia, H., Ayadi, M.A., Effect of different heating temperatures on foaming properties of camel milk proteins: a comparison with bovine milk proteins. Int. Dairy J., 104, 2020, 104643.
Lajnaf, R., Trigui, I., Samet-Bali, O., Attia, H., Ayadi, M.A., Comparative study on emulsifying and physico-chemical properties of bovine and camel acid and sweet wheys. J. Food Eng., 268, 2020, 109741.
Plessas, S., Bosnea, L., Alexopoulos, A., Bezirtzoglou, E., Potential effects of probiotics in cheese and yogurt production: a review. Eng. Life Sci. 12 (2012), 433–440.
Atik, D.S., Gürbüz, B., Bölük, E., Palabıyık, İ., Development of vegan kefir fortified with Spirulina platensis. Food Biosci., 42, 2021, 101050.
Terpou, A., Mantzourani, I., Galanis, A., Kanellaki, M., Bezirtzoglou, E., Bekatorou, A., Koutinas, A.A., Plessas, S., Employment of L. paracasei K5 as a novel potentially probiotic freeze-dried starter for feta-type cheese production. Microorganisms, 7, 2018, 3.
International, Official Methods of Analysis of AOAC International; AOAC International: Gaithersburg, MD, USA. 1995.
Masmoudi, M., Ammar, I., Ghribi, H., Attia, H., Physicochemical, radical scavenging activity and sensory properties of a soft cheese fortified with Arbutus unedo L. extract. Food Biosci., 35, 2020, 100579.
Bersuder, P., Hole, M., Smith, G., Antioxidants from a heated histidine-glucose model system. I: investigation of the antioxidant role of histidine and isolation of antioxidants by high-performance liquid chromatography. J. Am. Oil Chem. Soc. 75 (1998), 181–187.
Tseng, A., Zhao, Y., Wine grape pomace as antioxidant dietary fibre for enhancing nutritional value and improving storability of yogurt and salad dressing. Food Chem. 138 (2013), 356–365.
Salvador, A., Fiszman, S.M., Textural and sensory characteristics of whole and skimmed flavored set-type yogurt during long storage. J. Dairy Sci. 87 (2004), 4033–4041.
Felfoul, I., Jardin, J., Gaucheron, F., Attia, H., Ayadi, M.A., Proteomic profiling of camel and cow milk proteins under heat treatment. Food Chem. 216 (2017), 161–169.
Mohamed, H., Johansson, M., Lundh, Å., Nagy, P., Kamal-Eldin, A., Short communication: caseins and α-lactalbumin content of camel milk (Camelus dromedarius) determined by capillary electrophoresis. J. Dairy Sci. 103 (2020), 11094–11099.
Lajnaf, R., Attia, H., Ayadi, M.A., Effect of heat treatments on foaming and physico-chemical properties of bovine and camel sodium caseinate. J. Dairy Res., 2022, 1–5.
Merin, U., Bernstein, S., Yagil, R., Van Creveld, C., Lindner, P., Gollop, N., Short communication a comparative study of milk serum proteins in camel (Camelus dromedarius) and bovine colostrum. Livest. Prod. Sci. 67 (2001), 297–301.
El-Agamy, E.I., Nawar, M., Shamsia, S.M., Awad, S., Haenlein, G.F.W., Are camel milk proteins convenient to the nutrition of cow milk allergic children?. Small Rumin. Res. 82 (2009), 1–6.
Farah, Z., Rettenmaier, R., Atkins, D., Vitamin content of camel milk. Int. J. Vitam. Nutr. Res. 62 (1992), 30–33.
Bchir, B., Felfoul, I., Bouaziz, M.A., Gharred, T., Yaich, H., Noumi, E., Snoussi, M., Bejaoui, H., Kenzali, Y., Blecker, C., Attia, H., Investigation of physicochemical, nutritional, textural, and sensory properties of yoghurt fortified with fresh and dried Spirulina (Arthrospira platensis). Int. Food Res. J. 26 (2019), 1565–1576.
Bhagiel, I., Mustafa, E.A., Tabidi, M.M., Ahmed, M.E.M., Comparison between the physiochemical attributes of yogurt processed from camel milk and that processed from cow milk and the effect of storage period on pH and acidity. World J. Pharm. Pharm. Sci. 4 (2015), 1530–1540.
Khaskheli, M., Arain, M.A., Chaudhry, S., Soomro, A.H., Qureshi, T.A., Physico-chemical quality of camel milk. J. Agric. Soc. Sci. 2 (2005), 164–166.
Lee, W.-J., Lucey, J.A., Formation and physical properties of yogurt. Asian-Australasian J. Anim. Sci. 23 (2010), 1127–1136.
Ayyash, M., Al-Dhaheri, A.S., Al Mahadin, S., Kizhakkayil, J., Abushelaibi, A., In vitro investigation of anticancer, antihypertensive, antidiabetic, and antioxidant activities of camel milk fermented with camel milk probiotic: a comparative study with fermented bovine milk. J. Dairy Sci. 101 (2018), 900–911.
Soleymanzadeh, N., Mirdamadi, S., Kianirad, M., Antioxidant activity of camel and bovine milk fermented by lactic acid bacteria isolated from traditional fermented camel milk (Chal). Dairy Sci. Technol. 96 (2016), 443–457.
Ismaiel, M.M.S., El-Ayouty, Y.M., Piercey-Normore, M., Role of pH on antioxidants production by Spirulina (Arthrospira) platensis. Braz. J. Microbiol. 47 (2016), 298–304.
Shori, A.B., Camel milk as a potential therapy for controlling diabetes and its complications: a review of in vivo studies. J. Food Drug Anal. 23 (2015), 609–618.
Jung, F., Krüger-Genge, A., Waldeck, P., Küpper, J.-H., Spirulina platensis, a super food?. J. Cell. Biotechnol. 5 (2019), 43–54.
Abu-Lehia, I.H., Physical and chemical characteristics of camel milkfat and its fractions. Food Chem. 34 (1989), 261–271.
Mbye, M., Sobti, B., Al Nuami, M.K., Al Shamsi, Y., Al Khateri, L., Al Saedi, R., Saeed, M., Ramachandran, T., Hamed, F., Kamal-Eldin, A., Physicochemical properties, sensory quality, and coagulation behavior of camel versus bovine milk soft unripened cheeses. NFS J. 20 (2020), 28–36.
Barzegar, A., Yousefi, R., Sharifzadeh, A., Dalgalarrondo, M., Chobert, J.M., Ganjali, M.R., Norouzi, P., Ehsani, M.R., Niasari-Naslaji, A., Saboury, A.A., Haertlé, T., Moosavi-Movahedi, A.A., Chaperone activities of bovine and camel β-caseins: importance of their surface hydrophobicity in protection against alcohol dehydrogenase aggregation. Int. J. Biol. Macromol. 42 (2008), 392–399.
Lousada Falcão, R., Pinheiro, V., Ribeiro, C., Sousa, I., Raymundo, A., Nunes, M.C., Nutritional improvement of fresh cheese with microalga Chlorella vulgaris: impact on composition, structure and sensory acceptance. Food Technol. Biotechnol. 61 (2023), 259–270.
Bernaerts, T.M.M., Gheysen, L., Foubert, I., Hendrickx, M.E., Van Loey, A.M., The potential of microalgae and their biopolymers as structuring ingredients in food: a review. Biotechnol. Adv., 37, 2019, 107419.
