date seed oil; enrichment; margarine; oxidative stability; roasting
Abstract :
[en] Bakery margarine is one of the most commonly used fat products in the food industry. Its oxidative stability is a critical quality parameter, typically maintained using synthetic antioxidants such as tertiary butylhydroquinone (TBHQ) due to its strong antioxidant activity. However, excessive daily consumption of TBHQ has been linked to potential health risks, prompting increased interest in natural alternatives. In this work, oil extracted from roasted and unroasted date seeds were analyzed and compared for there phenolic composition and free radical scavenging capacity. In addition, bakery margarines prepared by formulation with the roasted and unroasted date seed oils (10 g/kg) were compared to margarine supplemented with the synthetic antioxidant TBHQ (200 m/kg) as a control. They were evaluated in terms of quality parameters, oxidative stability measured by induction time, peroxide value during accelerated storage for 10 weeks at 60°C, and fatty acid profile. The results showed that pre-treatment of the seeds by roasting significantly increased phytochemical content and antioxidant capacity. Margarine containing roasted
date seed extracts showed oxidation resistance similar to the control without altering its quality. However, it showed superior resistance properties and a longer induction time (increased from 24.6 to 31 h) compared to margarine containing unroasted
date seed oil. This study highlights, for the first time, the role of roasting in improving the quality of date seed oil. These results suggest that roasted date seed oil can effectively improve the shelf life and stability of margarine, offering a promising natural
alternative to synthetic antioxidants in food applications.
Disciplines :
Food science
Author, co-author :
Mayouf, Rahma; Laboratoire de Biochimie Biophysique Biomathématique et Scientométrie, Faculté Des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria
Hamitri-Guerfi, Fatiha; Laboratoire de Biochimie Biophysique Biomathématique et Scientométrie, Faculté Des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria
Benbouriche, Aicha; Laboratoire de Biochimie Biophysique Biomathématique et Scientométrie, Faculté Des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria
Ayadi, Mohamed ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Hadjal, M.; Laboratoire Central Des Corps Gras, Complexe Agro-Alimentaire de CEVITAL Agro-Industrie, Bejaia, Algeria
Zeroual, Brahim; Laboratoire Central Des Corps Gras, Complexe Agro-Alimentaire de CEVITAL Agro-Industrie, Bejaia, Algeria
Ouatmani, Toufik; Laboratoire Central Des Corps Gras, Complexe Agro-Alimentaire de CEVITAL Agro-Industrie, Bejaia, Algeria
Hamitri, Morad; Laboratoire Central Des Corps Gras, Complexe Agro-Alimentaire de CEVITAL Agro-Industrie, Bejaia, Algeria
Attia, Hamadi; Laboratoire Analyses, Valorisation et Sécurité Des Aliments (LAVASA), Ecole Nationale D'ingénieurs de Sfax, Université de Sfax, Sfax, Tunisie
Boulekbache-Makhlouf, Lila; Laboratoire de Biochimie Biophysique Biomathématique et Scientométrie, Faculté Des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria
Felfoul, Imène; Laboratoire Analyses, Valorisation et Sécurité Des Aliments (LAVASA), Ecole Nationale D'ingénieurs de Sfax, Université de Sfax, Sfax, Tunisie
Language :
English
Title :
Investigating the Impact of Roasted Date Seed (Phoenix dactylifera L.) Oil on the Quality and Oxidative Stability of Bakery Margarine
Publication date :
2025
Journal title :
Journal of the American Oil Chemists' Society
ISSN :
0003-021X
eISSN :
1558-9331
Publisher :
American Oil Chemists' Society Press, Champaign, United States - Illinois
Abd-Elkarim, N. A., E. A. Abdrabou, and A.-K. Osman. 2024. “Influence of Roasting and Microwave Heating on Nutritional Value and Bioactive Compounds of Siwi Date Seed.” Egyptian Journal of Food Science 52: 123–137. https://doi.org/10.21608/ejfs.2024.223982.1190.
Abid, Y., S. Azabou, M. Jridi, I. Khemakhem, M. Bouaziz, and H. Attia. 2017. “Storage Stability of Traditional Tunisian Butter Enriched With Antioxidant Extract From Tomato Processing By-Products.” Food Chemistry 233: 476–482. https://doi.org/10.1016/j.foodchem.2017.04.125.
Akinoso, R., E. O. Adegoroye, and M. S. Sanusi. 2023. “Effects of Roasting on Physicochemical Properties and Fatty Acids Composition of Okra Seed Oil.” Measurement: Food 9: 100076. https://doi.org/10.1016/j.meafoo.2023.100076.
Ali, R. F. M., and A. M. El-Anany. 2025. “Physico-Chemical Characteristics, Nutritional Quality, and Oxidative Stability of Cold-Pressed Chia Seed Oil, Date Palm Seed Oil, Along With Their Binary Mixtures.” Cogent Food & Agriculture 11, no. 1: 2466061. https://doi.org/10.1080/23311932.2025.2466061.
Ammar, I., H. Gharsallah, A. Ben Brahim, et al. 2021. “Optimization of Gluten-Free Sponge Cake Fortified With Whey Protein Concentrate Using Mixture Design Methodology.” Food Chemistry 343: 128457. https://doi.org/10.1016/j.foodchem.2020.128457.
Azizkhani, M., and P. Zandi. 2009. “Effects of Some Natural Antioxidants Mixtures on Margarine Stability.” International Journal of Food Engineering 3: 53–56.
Basuny, A. M. M., and M. A. Al-Marzooq. 2011. “Production of Mayonnaise From Date Pit Oil.” Food and Nutrition Sciences 2: 938–943. https://doi.org/10.4236/fns.2011.29128.
Belcadi-Haloui, R., A. Zekhnini, Y. El-Alem, and A. Hatimi. 2018. “Effects of Roasting Temperature and Time on the Chemical Composition of Argan Oil.” International Journal of Food Science 2018: 7683041. https://doi.org/10.1155/2018/7683041.
Benbouriche, A., H. Haddadi-Guemghar, M. Bachir-Bey, et al. 2022. “Improvement of Thermo-Resistance and Quality of Soybean Oil by Blending With Cold-Pressed Oils Using Simplex Lattice Mixture Design.” Oilseeds and Fats, Crops and Lipids 29: 33. https://doi.org/10.1051/ocl/2022025.
Benbouriche, A., H. Haddadi-Guemghar, D. Mehidi-Terki, L. Boulekbache-Makhlouf, and M. Bachir-Bey. 2023. “Enrichment of Soybean Oil With Carotenoids From Chili Paste By-Product: Enhancement of Quality, Oxidative Stability, and Thermo-Resistance.” Journal of Food Measurement and Characterization 18: 1107–1120. https://doi.org/10.1007/s11694-023-02239-2.
Bentayeb Ait Lounis, S., L. Mekimène, D. Mazi, et al. 2018. “Nutritional Quality and Safety of Algerian Margarines: Fatty Acid Composition, Oxidative Stability and Physicochemical Properties.” Mediterranean Journal of Nutrition and Metabolism 11: 331–342. https://doi.org/10.3233/MNM-18208.
Besbes, S., C. Blecker, C. Deroanne, G. Lognay, N. E. Drira, and H. Attia. 2004. “Quality Characteristics and Oxidative Stability of Date Seed Oil During Storage.” Food Science and Technology International 10: 333–338. https://doi.org/10.1177/1082013204047777.
Bolini, H. M. A., A. C. Medeiros, C. T. M. Pereira, et al. 2023. “Consumer Acceptance Studies of Margarine to Guide Product Development in the Food Industry.” Food 13: 116. https://doi.org/10.3390/foods13010116.
Chbani, M., B. Matthaus, Z. Charrouf, et al. 2020. “Characterization of Phenolic Compounds Extracted From Cold Pressed Cactus (Opuntia ficus-indica L.) Seed Oil and the Effect of Roasting on Their Composition.” Food 9: 1098. https://doi.org/10.3390/foods9081098.
Chen, J., Y. Zhao, R. Wu, et al. 2023. “Changes in the Quality of High-Oleic Sunflower Oil During the Frying of Shrimp (Litopenaeus Vannamei).” Food 12: 1332. https://doi.org/10.3390/foods12061332.
Cheong, L. Z., C. P. Tan, K. Long, M. Suria Affandi Yusoff, and O. M. Lai. 2009. “Physicochemical, Textural and Viscoelastic Properties of Palm Diacylglycerol Bakery Margarine During Storage.” Journal of the American Oil Chemists' Society 86: 723–731.
Ciou, J.-Y., H.-C. Chen, C.-W. Chen, and K.-M. Yang. 2021. “Relationship Between Antioxidant Components and Oxidative Stability of Peanut Oils as Affected by Roasting Temperatures.” Agriculture 11: 300. https://doi.org/10.3390/agriculture11040300.
Codex Alimentarius Commission. 1999. Codex Standards for Fats and Oils From Vegetable Sources. Codex Standard for Named Vegetable Oils. Codex Alimentarius Commission (CODEX-STAN 210–1999).
Das, M., and A. Das. 2024. “A Comprehensive Review on Strategies for Replacing Saturated Fats in Bakery Products.” Discover Food 4: 156. https://doi.org/10.1007/s44187-024-00240-2.
Devi, A., and B. Khatkar. 2017. “Thermo-Physical Properties of Fats and Oils.” International Journal of Engineering Research & Technology 7: 45.
Dollah, S., K. F. Chai, S. M. Abdulkarim, and H. M. Ghazali. 2020. “Comparative Study of Table Margarine Prepared From Moringa oleifera Seed Oil-Palm Stearin Blend and Commercial Margarines: Composition, Thermal, and Textural Properties.” European Journal of Lipid Science and Technology 122: 1900428. https://doi.org/10.1002/ejlt.201900428.
El Harkaoui, S., K. N'diaye, S. Gharby, et al. 2024. “Insights Into Date Seed Oil Composition: Geographical Variability and Potential Applications.” European Journal of Lipid Science and Technology 126: e2400061. https://doi.org/10.1002/ejlt.202400061.
Ergönül, P. G. 2013. “Solid Fat Contents and Instrumental Textural Attributes of Margarines Sold in Turkish Market.” Quality Assurance & Safety of Crops and Food 5, no. 2: 157–161. https://doi.org/10.3920/QAS2012.0122.
Farhoosh, R. 2007. “The Effect of Operational Parameters of the Rancimat Method on the Determination of the Oxidative Stability Measures and Shelf-Life Prediction of Soybean Oil.” Journal of the American Oil Chemists' Society 84: 205–209. https://doi.org/10.1007/s11746-006-1030-4.
Fernandez-Salguero, J., R. Gomez, and M. Carmona. 1993. “Analysis Water Activity in Selected High-Moisture Foods.” Journal of Food Composition and Analysis 6: 364–369. https://doi.org/10.1006/jfca.1993.1040.
Fikry, M., Y. Aniza Yusof, A. M. Al-Awaadh, et al. 2019. “Kinetics Modelling of the Colour, Hardness, Grinding Energy Consumption and Oil Yield Changes During the Conventional Roasting of Palm Date Seeds.” Food Science and Technology Research 25: 351–362. https://doi.org/10.3136/fstr.25.351.
Fruehwirth, S., S. Egger, D. Kurzbach, et al. 2021. “Ingredient-Dependent Extent of Lipid Oxidation in Margarine.” Antioxidants 10: 105. https://doi.org/10.3390/antiox10010105.
Gao, P., Y. Cao, R. Liu, Q. Jin, and X. Wang. 2018. “Phytochemical Content, Minor-Constituent Compositions, and Antioxidant Capacity of Screw-Pressed Walnut Oil Obtained From Roasted Kernels.” European Journal of Lipid Science and Technology 121, no. 1: 1800292. https://doi.org/10.1002/ejlt.201800292.
Gerzhova, A., M. Mondor, M. Benali, M. Aider, and Technology. 2016. “Incorporation of Canola Proteins Extracted by Electroactivated Solutions in Gluten-Free Biscuit Formulation of Rice–Buckwheat Flour Blend: Assessment of Quality Characteristics and Textural Properties of the Product.” International Journal of Food Sciences and Nutrition 51: 814–827. https://doi.org/10.1111/ijfs.13034.
Ghelichi, S., M. Hajfathalian, B. Yesiltas, A. M. Sorensen, P. J. Garcia-Moreno, and C. Jacobsen. 2023. “Oxidation and Oxidative Stability in Emulsions.” Comprehensive Reviews in Food Science and Food Safety 22: 1864–1901. https://doi.org/10.1111/1541-4337.13134.
Golshan Tafti, A., N. Solaimani Dahdivan, and S. Yasini Ardakani. 2017. “Palm Stearin as Low Trans Hard Stock for Margarine.” International Food Research Journal 24: 821–827.
Harkat, H., R. Bousba, C. Benincasa, et al. 2022. “Assessment of Biochemical Composition and Antioxidant Properties of Algerian Date Palm (Phoenix Dactylifera L.) Seed Oil.” Plants (Basel) 11: 381. https://doi.org/10.3390/plants11030381.
ISO-15648. 2004. Fats and Butter-Determination of Salt Content. ISO.
ISO-3960. 2007. Animal and Vegetable Fats and Oils–Determination of Peroxide Value. ISO.
ISO-5509. 2000. Animal and Vegetable Fats and Oils–Preparation of Methyl Esters of Fatty Acids. ISO.
ISO-6321. 2002. Animal and Vegetable Fats and Oils-Determination of Melting Point in Open Capillary Tube (Slip Point). ISO.
ISO-660. 2009. Animal and Vegetable Fats and Oils-Determination of Acid Value and Acidity. ISO.
ISO-662. 1998. Animal and Vegetable Fats and Oils. Determination of Moisture Content and Volatile Matter. ISO.
ISO-6886. 2006. Animal and Vegetable Fats and Oils-Determination of Oxidation Stability (Accelerated Oxidation Test). ISO.
ISO-8292-1. 2008. Animal and Vegetable Fats and Oils- Determination of Solid Fat Content by Pulsed NMR Part 1-Direct Method. ISO.
Jacobsen, C. 2016. “Oxidative Stability and Shelf Life of Food Emulsions.” In Oxidative Stability and Shelf Life of Foods Containing Oils and Fats, 287–312. Elsevier Inc.
Jeyarani, T., S. Subramanian, R. Sneha, M. Sudha, and P. Negi. 2015. “Characterisation of Mango Kernel Fat and Preparation of Tran's-Free Margarine for Use in Muffins.” Journal of Nutrition & Food Sciences 5: 357. https://doi.org/10.4172/2155-9600.1000357.
Kaanin-Boudraa, G., F. Hamitri-Guerfi, L. Harfi, et al. 2023. “Physicochemical Characterization and Antioxidant Capacity of the Extracted Oil From Date Pits and Its Effect on Storage Stability of Margarine.” North African Journal of Food and Nutritional Research 7: 54–67. https://doi.org/10.51745/najfnr.7.16.54-67.
Karrar, E., I. A. Mohamed Ahmed, M. F. Manzoor, et al. 2021. “Effect of Roasting Pretreatment on Fatty Acids, Oxidative Stability, Tocopherols, and Antioxidant Activity of Gurum Seeds Oil.” Biocatalysis and Agricultural Biotechnology 34: 102022. https://doi.org/10.1016/j.bcab.2021.102022.
Liu, C., J. Li, Y. Bi, X. Wang, S. Sun, and G. Yang. 2016. “Thermal Losses of Tertiary Butylhydroquinone (TBHQ) and Its Effect on the Qualities of Palm Oil.” Journal of Oleo Science 65: 739–748. https://doi.org/10.5650/jos.ess16041.
Lopez, P. L., M. A. Marchesino, N. R. Grosso, and R. H. Olmedo. 2022. “Comparative Study of Accelerated Assays for Determination of Equivalent Days in the Shelf Life of Roasted High Oleic Peanuts: Chemical and Volatile Oxidation Indicators in Accelerated and Room Temperature Conditions.” Food Chemistry 373: 131479. https://doi.org/10.1016/j.foodchem.2021.131479.
López, P. L., N. S. Juncos, N. R. Grosso, and R. H. Olmedo. 2022. “Minthostachys Mollis Essential Oil and Its Combination With Tert-Butylhydroquinone for Control of Lipid Oxidation.” European Journal of Lipid Science and Technology 124, no. 11: 2200081. https://doi.org/10.1002/ejlt.202200081.
Miskandar, M. S., Y. C. Man, M. S. A. Yusoff, and R. A. Rahman. 2005. “Quality of Margarine: Fats Selection and Processing Parameters.” Asia Pacific Journal of Clinical Nutrition 14: 387–395.
Mohamed Ahmed, I. A., M. Musa Özcan, N. Uslu, et al. 2020. “Effect of Microwave Roasting on Color, Total Phenol, Antioxidant Activity, Fatty Acid Composition, Tocopherol, and Chemical Composition of Sesame Seed and Oils Obtained From Different Countries.” Journal of Food Processing & Preservation 44: e14807. https://doi.org/10.1111/jfpp.14807.
Mohd Jaih, A., R. A. Rahman, A. Razis, A. Ariffin, A. Al-Awaadh, and N. Suleiman. 2019. “Fatty Acid, Triacylglycerol Composition and Antioxidant Properties of Date Seed Oil.” International Food Research Journal 26: 517–527. https://doi.org/10.13140/RG.2.2.28516.22406.
Moure, A., J. M. Cruz, D. Franco, et al. 2001. “Natural Antioxidants From Residual Sources.” Food Chemistry 72: 145–171. https://doi.org/10.1016/S0308-8146(00)00223-5.
Nenadis, N., A. Mastralexi, M. Z. Tsimidou, et al. 2018. “Toward a Harmonized and Standardized Protocol for the Determination of Total Hydroxytyrosol and Tyrosol Content in Virgin Olive Oil (VOO). Extraction Solvent.” European Journal of Lipid Science and Technology 120: 1800099. https://doi.org/10.1002/ejlt.201800099.
Ouahrani, S., S. Casal, M. Bachir-Bey, and F. Zaidi. 2022. “Impact of Moringa oleífera Leaves Extract in the Stabilization of Margarine Under Accelerated Storage.” Journal of Food Measurement and Characterization 17: 1455–1466. https://doi.org/10.1007/s11694-022-01714-6.
Ouahrani, S., D. A. Tzompa-Sosa, K. Dewettinck, and F. Zaidi. 2022. “Oxidative Stability, Structural, and Textural Properties of Margarine Enriched With Moringa Oleifera Leaves Extract.” Journal of the American Oil Chemists' Society 99: 485–499. https://doi.org/10.1002/aocs.12586.
Pădureţ, S. 2022. “The Quantification of Fatty Acids, Color, and Textural Properties of Locally Produced Bakery Margarine.” Applied Sciences 12: 1731. https://doi.org/10.3390/app12031731.
Palanna, M., M. Itagi, L. K. Sannegowda, et al. 2025. “A Bio-Mimicking Cobalt Tetramenthol-Substituted Phthalocyanine-Based Electrochemical Sensor for Selective and Sensitive Detection of Tert-Butylhydroquinone.” Journal of Materials Chemistry B 13: 4188–4200. https://doi.org/10.1039/d4tb02043j.
Paranthaman, R., P. P. Kumar, and S. Kumaravel. 2012. “HPLC and HPTLC Determination of Caffeine in Raw and Roasted Date Seeds (Phoenix Dactylifera L).” Scientific Reports 1: 1–4. https://doi.org/10.4172/scientificreports.249.
Pietinen, P., L. M. Valsta, T. Hirvonen, and H. Sinkko. 2008. “Labelling the Salt Content in Foods: A Useful Tool in Reducing Sodium Intake in Finland.” Public Health Nutrition 11: 335–340. https://doi.org/10.1017/S1368980007000249.
Potočnik, T., M. Rak Cizej, and I. J. Košir. 2018. “Influence of Seed Roasting on Pumpkin Seed Oil Tocopherols, Phenolics and Antiradical Activity.” Journal of Food Composition and Analysis 69: 7–12. https://doi.org/10.1016/j.jfca.2018.01.020.
Rakhshanda, N., M. Mehmood, K. Mahmood, A. Sajjad, S. Munir, and N. Akbar. 2020. “Physiochemical Properties and Total Phenolic Content of Date Palm (Phoenix Sylvestris (L.) Roxb) Seed Oil Grown in District Panjgur, Pakistan.” International Journal of Biosciences 16, no. 2: 272–278. https://doi.org/10.12692/ijb/16.2.272-278.
Rice-Evans, C., N. Miller, and G. J. T. I. P. S. Paganga. 1997. “Antioxidant Properties of Phenolic Compounds.” Trends in Plant Science 2: 152–159.
Sahri, M. M., and N. A. Idris. 2010. “Palm Stearin as Low Trans Hard Stock for Margarine.” Sains Malaysiana 39: 821–827.
Samet-Bali, O., M. Ayadi, and H. Attia. 2009. “Traditional Tunisian Butter: Physicochemical and Microbial Characteristics and Storage Stability of the Oil Fraction.” LWT – Food Science and Technology 42: 899–905. https://doi.org/10.1016/j.lwt.2008.11.007.
Shahidi, F., and Y. J. C. S. R. Zhong. 2010. “Lipid Oxidation and Improving the Oxidative Stability.” Chemical Society Reviews 39: 4067–4079. https://doi.org/10.1039/B922183M.
Stefani Juncos, N., C. Florencia Cravero, N. Rubén Grosso, and O. R. Horacio. 2024. “Integral Oxidation Value Used as a New Oxidation Indicator for Evaluation of Advanced Stages of Oxidative Processes: Intox Value.” Microchemical Journal 204: 111186. https://doi.org/10.1016/j.microc.2024.111186.
Subhash, A. J., G. B. Bamigbade, and M. Ayyash. 2024. “Current Insights Into Date By-Product Valorization for Sustainable Food Industries and Technology.” Sustainable Food Technology 2, no. 2: 331–361. https://doi.org/10.1039/D3FB00224A.
Suri, K., B. Singh, and A. Kaur. 2022. “Impact of Microwave Roasting on Physicochemical Properties, Maillard Reaction Products, Antioxidant Activity and Oxidative Stability of Nigella Seed (Nigella Sativa L.) oil.” Food Chemistry 368: 130777. https://doi.org/10.1016/j.foodchem.2021.130777.
Suri, K., B. Singh, A. Kaur, M. P. Yadav, and N. Singh. 2020. “Influence of Microwave Roasting on Chemical Composition, Oxidative Stability and Fatty Acid Composition of Flaxseed (Linum Usitatissimum L.) oil.” Food Chemistry 326: 126974. https://doi.org/10.1016/j.foodchem.2020.126974.
Taha, E., N. Abd-Elkarim, and Z. Ahmed. 2019. “Date Seed Oil as a Potential Natural Additive to Improve Oxidative Stability of Edible Vegetable Oils.” Egyptian Journal of Food Science 47: 105–113. https://doi.org/10.21608/ejfs.2019.13050.1007.
United States Food and Drug Administration. 2024. Code of Federal Regulations, Title 21, Part 172. U.S. Government Publishing Office.
Vucic, V., A. Arsic, S. Petrovic, S. Milanovic, M. Gurinovic, and M. Glibetic. 2015. “Trans Fatty Acid Content in Serbian Margarines: Urgent Need for Legislative Changes and Consumer Information.” Food Chemistry 185: 437–440. https://doi.org/10.1016/j.foodchem.2015.04.018.
Vujasinovic, V., S. Djilas, E. Dimic, Z. Basic, and O. Radocaj. 2012. “The Effect of Roasting on the Chemical Composition and Oxidative Stability of Pumpkin Oil.” European Journal of Lipid Science and Technology 114: 568–574. https://doi.org/10.1002/ejlt.201100158.
Wannes, W. A., and B. Marzouk. 2016. “Characterization of Myrtle Seed (Myrtus communis Var. Baetica) as a Source of Lipids, Phenolics, and Antioxidant Activities.” Journal of Food and Drug Analysis 24: 316–323. https://doi.org/10.1016/j.jfda.2015.11.001.
Yao, X.-H., Y.-S. Shen, R.-Z. Hu, et al. 2020. “The Antioxidant Activity and Composition of the Seed Oil of Mulberry Cultivars.” Food Bioscience 37: 100709. https://doi.org/10.1016/j.fbio.2020.100709.
Yilmaz, E. G., and A. Yorulmaz. 2023. “Improvement in Frying Stability of Safflower Oil by Blending With Refined Olive Pomace Oil During Deep Fat Frying.” Journal of Oleo Science 72: 901–910. https://doi.org/10.5650/jos.ess23016.
