The morphology and molecular mechanisms of enhanced olfaction in the grain aphid Sitobion miscanthi

Aphid; Odorant binding protein (OBP); Olfactory sensillum; Parallel reaction monitoring (PRM); Wing phenotype; Aromatization; Sensory perception; limonene; pheromone; pinene; Miscanthus; Odorant binding protein; Odorant-binding proteins; Olfactory sensilla; Parallel reaction monitoring; Parallel reactions; Plant volatiles; Reaction monitoring; animal experiment; animal model; aphid; Article; binding affinity; controlled study; electroencephalography; expression vector; female; gene expression; high performance liquid chromatography; liquid chromatography-mass spectrometry; mass spectrometry; morphology; nonhuman; olfactory system; parallel reaction monitoring; procedures; protein analysis; proteomics; secondary rhinarium; sense organ; Sitobion miscanthi; Triticum aestivum; ultra performance liquid chromatography; Ligands

Abstract :

[en] Winged aphids develop more sensitive olfaction than the wingless phenotype to identify potential habitat from afar. Two types of olfactory sensilla, primary rhinarium (PRh) and secondary rhinarium (SRh) are responsible for aphid olfactory perception, of which, SRh is involved in the perception of both E-β-farnesene (EBF) and plant volatiles. Odorant binding proteins (OBPs) play a vital role in the response of insect olfactory nerves located in the rhinarium to external odor stimuli. However, the role of SRhs in winged aphids have received little attention to date and the underlying mechanisms of how OBPs work for the olfactory enhancement in winged aphids are unclear. Here, we compared and analyzed the number of SRhs on the antennae of winged and wingless aphids in the grain aphid Sitobion miscanthi, then highly expressed OBPs in antennae of winged aphids were screened based on both transcriptomic and proteomic data and identified by parallel reaction monitoring parallel reaction monitoring (PRM). Furthermore, the affinity of highly expressed OBPs was tested. The results showed that winged aphids have more SRhs, and the three highly expressed OBPs, SmisOBP6/7/10, in winged antennae shared broad and overlapped ligands spectra with plant volatiles and pheromones. Furthermore, OBP9, as an OBP with high expression in antennae, not only binds EBF, but also shows extensive affinity to various ligand types. Our results highlight the importance of the higher number of SRhs as the morphological basis of enhanced olfactory perception in winged aphid and further indicates the involvement of OBP6/7/10 as the molecular support of this process. © 2024

Disciplines :

Entomology & pest control

Author, co-author :

Fan, Jia; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Jiang, Xin ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Li, Qian; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Yu, Miaomiao; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Zhang, Siyu; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Xue, Wenxin; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs

Su, Changqing; Center for Wetland Conservation and Research, Hengshui University, Hebei Province, Hengshui, 053000, China ; Collaborative Innovation Center for Wetland Conservation and Green Development of Hebei Province, Hengshui, 053000, China

Aradottir, Gudbjorg Inga; Mamore Research and Innovation Limited, Harpenden, Hertfordshire, United Kingdom

Liu, Yanxia; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Zhang, Yong; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Chen, Julian; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Language :

English

Title :

The morphology and molecular mechanisms of enhanced olfaction in the grain aphid Sitobion miscanthi

Publication date :

February 2025

Journal title :

International Journal of Biological Macromolecules

ISSN :

0141-8130

eISSN :

1879-0003

Publisher :

Elsevier

Volume :

289

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

NSCF - National Natural Science Foundation of China

Available on ORBi :

since 25 July 2025

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