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Author Correction: Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae (Scientific Reports, (2017), 7, 1, (15911), 10.1038/s41598-017-16092-z)
Zhang, Yong; Fan, Jia; Sun, Jingrui et al.
2020In Scientific Reports, 10 (1)
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Keywords :
Aphid saliva, sitobion avenue, salivary glands, proteins,
Abstract :
[en] Aphid saliva plays important roles in aphid-host interactions, such as assisting aphid digestion, detoxification, activating or suppressing plant defenses. The grain aphid, Sitobion avenae, is one of the most devastating pests of cereals worldwide. In this study, we performed the transcriptome analysis of salivary glands of S. avenae. A total of 33,079 assembled unigenes were identified in the salivary glands of aphids. Of the all obtained unigenes, 15,833(47.86%) and 10,829(32.73%) unigenes showed high similarity to known proteins in Nr and Swiss-Prot databases respectively. 526 unigenes were predicted to encode secretory proteins, including some digestive and detoxifying enzymes and potential effectors. The RT-PCR and RT-qPCR results showed that all of the 15 most highly expressed putative secretory proteins specifically expressed in salivary glands. Interestingly, 11 of the 15 most highly expressed putative secretory proteins were still not matched to function-known proteins. We also detected the expression of 9 interested putative secretory proteins in aphid different tissues, including some digestive and detoxifying enzymes, effectors and Ca2+ binding proteins. The results showed that only glutathione-S-transferase 1 was specifically expressed in salivary glands. These findings provide a further insight into the identification of potential effectors involving in aphid-cereals interactions. amendment to this paper has been published and can be accessed via a link at the top of the paper. © 2020, The Author(s).
Disciplines :
Entomology & pest control
Author, co-author :
Zhang, Yong;  State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China ; Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, 5030, Belgium
Fan, Jia;  State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Sun, Jingrui;  State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Francis, Frédéric  ;  Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Chen, Julian;  State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Language :
English
Title :
Author Correction: Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae (Scientific Reports, (2017), 7, 1, (15911), 10.1038/s41598-017-16092-z)
Publication date :
July 2020
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature
Volume :
10
Issue :
1
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NSCF - National Natural Science Foundation of China
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