HS‐GC‐IMS; HS‐SPME‐GC‐MS; Tibetan sheep; VOCs; Biotechnology; Food Science; Agronomy and Crop Science; Nutrition and Dietetics
Abstract :
[en] [en] BACKGROUD: To clarify the variations in flavor characteristics among various Tibetan sheep breeds (Gangba, Huoerba, Sewa, Duoma and Awang) across different mutton portions (M. longissimus thoracis et lumborum, topside, thick flank and shoulder fillet), HS-SPME-GC-MS and HS-GC-IMS were performed with multivariate statistical analysis to characterize the volatile organic compounds (VOCs) in Tibetan sheep mutton.
RESULTS: The results identified 89 and 75 VOCs using HS-SPME-GC-MS and HS-GC-IMS, respectively, with aldehydes, ketones and alcohols as the primary compound classes. The main flavor characteristic substances were 1-octen-3-ol, 3-hydroxy-2-butanone, 2,3-pentanedione, acetone, octanal, 2-pentylfuran, nonanal, hexanal, 1-hexanol, 2-butanone, 1-pentanol, heptanal, 2-pentanone, 1-penten-3-ol and benzaldehyde, contributing notes of fruity, floral, grassy, caramel, baking and creamy aromas. The contributing compounds of odor activity value > 1 were successfully determined, enabling the characterization of Tibetan sheep mutton flavor profiles. Fingerprints were applied to distinguish common and unique VOCs across different mutton portions from the studied breeds. Samples were effectively categorized by principal component analysis and partial least squares discriminant analysis (PLS-DA) according to their aroma characteristics, and a stable model (variable importance in projection (VIP)) was established through PLS-DA, highlighting hexanal, 1-hexanol, 2-butanone, 1-pentanol, heptanal, 1-octen-3-ol, 3-hydroxy-2-butanone, 2-pentanone, octanal, ethanol, acetone, 1-penten-3-ol, 2,3-pentanedione and acetic acid with VIP > 1.
CONCLUSION: The findings reveal the VOC profiles of various mutton portions of Tibetan sheep breeds, offering insights for product development and enhancing the understanding of flavor in Tibetan sheep meat products.
Disciplines :
Food science
Author, co-author :
Xu, Yuqian ; Université de Liège - ULiège > TERRA Research Centre ; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China
Yang, Qingfeng; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China
Zheng, Xiaochun; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China
Yang, Huiguo; Xinjiang Uyghur Autonomous Region Academy of Animal Science, Urumqi, China
Schroyen, Martine ; Université de Liège - ULiège > TERRA Research Centre > Animal Sciences (AS)
Zhang, Dequan ; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China
Hou, Chengli ; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China
Language :
English
Title :
Identification of volatile flavor substances in four key muscle portions of five Tibetan sheep breeds.
Publication date :
01 May 2025
Journal title :
Journal of the Science of Food and Agriculture
ISSN :
0022-5142
eISSN :
1097-0010
Publisher :
John Wiley and Sons Ltd, England
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
This work was supported by the National Key R&D Program of China (2021YFD1600700).
Fan Y, Liang Y, Deng K, Zhang Z, Zhang G, Zhang Y et al., Analysis of DNA methylation profiles during sheep skeletal muscle development using whole-genome bisulfite sequencing. BMC Genomics 21:327 (2020).
Zhang Q, Que M, Li W, Gao S, Tan X and Bu D, Gangba sheep in the Tibetan plateau: validating their unique meat quality and grazing factor analysis. J Environ Sci 101:117–122 (2021).
Zhang X, Han L, Hou S, Raza SHA, Wang Z, Yang B et al., Effects of different feeding regimes on muscle metabolism and its association with meat quality of Tibetan sheep. Food Chem 374:131611 (2022).
Hastie M, Ha M, Jacob RH, Hepworth G, Torrico DD and Warner RD, High consumer acceptance of mutton and the influence of ageing method on eating quality. Meat Sci 189:108813 (2022).
Jia W, Di C and Shi L, Applications of lipidomics in goat meat products: biomarkers, structure, nutrition interface and future perspectives. J Proteomics 270:104753 (2023).
Wang Q, Li L, Ding W, Zhang D, Wang J, Reed K et al., Adulterant identification in mutton by electronic nose and gas chromatography-mass spectrometer. Food Control 98:431–438 (2019).
Liu H, Hui T, Zheng X, Li S, Wei X, Li P et al., Characterization of key lipids for binding and generating aroma compounds in roasted mutton by UPLC-ESI-MS/MS and orbitrap Exploris GC. Food Chem 374:131723 (2022).
Li J, Yang Y, Tang C, Yue S, Zhao Q, Li F et al., Changes in lipids and aroma compounds in intramuscular fat from Hu sheep. Food Chem 383:132611 (2022).
Pereira PFM, de Sousa Picciani PH, Calado V and Tonon RV, Electrical gas sensors for meat freshness assessment and quality monitoring: a review. Trends Food Sci Technol 118:36–44 (2021).
Yao W, Ma S, Wu H, Liu D, Liu J and Zhang M, Flavor profile analysis of grilled lamb seasoned with classic salt, chili pepper, and cumin (Cuminum cyminum) through HS-SPME-GC-MS, HS-GC-IMS, E-nose techniques, and sensory evaluation on Sonit sheep. Food Chem 454:139514 (2024).
Yao W, Wu H, Cai Y, Chen Y, Liu D and Zhang M, Comprehensive analysis of geographical impact on flavor profiles of braised chicken across eastern, central, and western China using GC-IMS, E-nose techniques and sensory evaluation. Food Chem Adv 5:100808 (2024).
Zhou B, Zhao X, Laghi L, Jiang X, Tang J, Du X et al., Insights into the flavor profile of yak jerky from different muscles based on electronic nose, electronic tongue, gas chromatography–mass spectrometry and gas chromatography–ion mobility spectrometry. Foods 13:2911 (2024).
Wang J, Xu Z, Zhang H, Wang Y, Liu X, Wang Q et al., Meat differentiation between pasture-fed and concentrate-fed sheep/goats by liquid chromatography quadrupole time-of-flight mass spectrometry combined with metabolomic and lipidomic profiling. Meat Sci 173:108374 (2021).
Zhang C, Zhang J, Tuersuntuoheti M, Zhou W, Han Z, Li X et al., Landscape genomics reveals adaptive divergence of indigenous sheep in different ecological environments of Xinjiang, China. Sci Total Environ 904:166698 (2023).
Sun X, Yu Y, Wang Z, Akhtar KH, Saleh ASM, Li W et al., Insights into flavor formation of braised chicken: based on E-nose, GC–MS, GC-IMS, and UPLC-Q-Exactive-MS/MS. Food Chem 448:138972 (2024).
Nie R, Wang Z, Liu H, Wei X, Zhang C and Zhang D, Investigating the impact of lipid molecules and heat transfer on aroma compound formation and binding in roasted chicken skin: a UHPLC-HRMS and GC-O-MS study. Food Chem 447:138877 (2024).
Li C, Al-Dalali S, Wang Z, Xu B and Zhou H, Investigation of volatile flavor compounds and characterization of aroma-active compounds of water-boiled salted duck using GC–MS–O, GC–IMS, and E-nose. Food Chem 386:132728 (2022).
Zhu X, Healy LE, Sevindik O, Sun D-W, Selli S, Kelebek H et al., Impacts of novel blanching treatments combined with commercial drying methods on the physicochemical properties of Irish brown seaweed Alaria esculenta. Food Chem 369:130949 (2022).
Zhao J, Wang M, Xie J, Zhao M, Hou L, Liang J et al., Volatile flavor constituents in the pork broth of black-pig. Food Chem 226:51–60 (2017).
Hao Y, Kang J, Guo Y, Meng L, Li Z and Qin X, Bacterial interactions mediated by acetic acid and their impact on flavor profile during acetic acid fermentation stage of Shanxi aged vinegar. Food Biosci 64:105996 (2025).
Huang Q, Dong K, Wang Q, Huang X, Wang G, An F et al., Changes in volatile flavor of yak meat during oxidation based on multi-omics. Food Chem 371:131103 (2022).
Wen R, Hu Y, Zhang L, Wang Y, Chen Q and Kong B, Effect of NaCl substitutes on lipid and protein oxidation and flavor development of Harbin dry sausage. Meat Sci 156:33–43 (2019).
Schuster L, Franke C, Silcock P, Beauchamp J and Bremer PJ, Development of a novel sample reuse approach to measure the impact of lean meat, bone and adipose tissue on the development of volatiles in vacuum-packed chilled lamb stored at 2 °C for 15 days. Meat Sci 145:31–39 (2018).
Jiang H, Dai A, Yan L, Zhang Z, Ding B, Bai J et al., Analysis of volatile organic compounds (VOCs) in yak ghee from different pastoral areas of China based on GC-IMS. Int Dairy J 160:106098 (2024).
Wang F, Gao Y, Wang H, Xi B, He X, Yang X et al., Analysis of volatile compounds and flavor fingerprint in Jingyuan lamb of different ages using gas chromatography–ion mobility spectrometry (GC–IMS). Meat Sci 175:108449 (2021).
Sun A, Wu W, Soladoye OP, Aluko RE, Bak KH, Fu Y et al., Maillard reaction of food-derived peptides as a potential route to generate meat flavor compounds: a review. Food Res Int 151:110823 (2022).
Mariutti LRB and Bragagnolo N, Influence of salt on lipid oxidation in meat and seafood products: a review. Food Res Int 94:90–100 (2017).
Chen X, Luo J, Lou A, Wang Y, Yang D and Shen QW, Duck breast muscle proteins, free fatty acids and volatile compounds as affected by curing methods. Food Chem 338:128138 (2021).
Shen C, Cai Y, Wu X, Gai S, Wang B and Liu D, Characterization of selected commercially available grilled lamb shashliks based on flavor profiles using GC-MS, GC × GC-TOF-MS, GC-IMS, E-nose and E-tongue combined with chemometrics. Food Chem 423:136257 (2023).
Zhang L, Badar IH, Chen Q, Xia X, Liu Q and Kong B, Changes in flavor, heterocyclic aromatic amines, and quality characteristics of roasted chicken drumsticks at different processing stages. Food Control 139:109104 (2022).
Liang M, Zhang Z, Wu Y, Wang R and Liu Y, Comparison of Amomum tsaoko Crevost et Lemaire from four regions via headspace solid-phase microextraction: variable optimization and volatile characterization. Ind Crops Prod 191:115924 (2023).