[en] Chemical pollution in coastal waters, particularly from agricultural runoff organophosphates, poses a significant threat to marine ecosystems, including coral reefs. Pollutants such as chlorpyrifos (CPF) are widely used in agriculture and have adverse effects on marine life and humans. In this paper, we investigate the impact of CPF on the metamorphosis of a coral reef fish model, the clownfish Amphiprion ocellaris, focusing on the disruption of thyroid hormone (TH) signalling pathways. Our findings reveal that by reducing TH levels, CPF exposure impairs the formation of characteristic white bands in clownfish larvae, indicative of metamorphosis progression. Interestingly, TH treatment can rescue these effects, establishing a direct causal link between CPF effect and TH disruption. The body shape changes occurring during metamorphosis are also impacted by CPF exposure, shape changes are less advanced in CPF-treated larvae than in control conditions. Moreover, transcriptomic analysis elucidates CPF's effects on all components of the TH signalling pathway. Additionally, CPF induces systemic effects on cholesterol and vitamin D metabolism, DNA repair, and immunity, highlighting its broader TH-independent impacts. Pollutants are often overlooked in marine ecosystems, particularly in coral reefs. Developing and enhancing coral reef fish models, such as Amphiprion ocellaris (Cuvier, 1830), offers a more comprehensive understanding of how chemical pollution affects these ecosystems. This approach provides new insights into the complex mechanisms underlying CPF toxicity during fish metamorphosis, shedding light on the broader impact of environmental pollutants on marine organisms.
Reynaud, Mathieu ; Marine Eco-Evo-Devo Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Japan ; PSL Université Paris, EPHE-UPVD-CNRS, UAR3278 CRIOBE, French Polynesia
Vianello, Stefano ; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
Lee, Shu-Hua; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
Salis, Pauline ; Sorbonne Université, CNRS, Biologie Intégrative des Organisms Marins, BIOM, Observatoire Océanologique, France
Wu, Kai; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
Frederich, Bruno ; Université de Liège - ULiège > Freshwater and OCeanic science Unit of reSearch (FOCUS)
Lecchini, David; PSL Université Paris, EPHE-UPVD-CNRS, UAR3278 CRIOBE, French Polynesia ; Laboratoire d'Excellence “CORAIL”, Perpignan, France
Besseau, Laurence; Sorbonne Université, CNRS, Biologie Intégrative des Organisms Marins, BIOM, Observatoire Océanologique, France
Roux, Natacha; Computational Neuroethology Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Japan
Laudet, Vincent; Marine Eco-Evo-Devo Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Japan ; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan ; CNRS IRL 2028 “Eco-Evo-Devo of Coral Reef Fish Life Cycle” (EARLY, France
Language :
English
Title :
The multi-level effect of chlorpyrifos during clownfish metamorphosis
Genomics Research Center, Academia Sinica ANR - French National Research Agency Academia Sinica JSPS - Japan Society for the Promotion of Science
Funding text :
We thank the High Throughput Genomics Core of the Biodiversity Research Center at Academia Sinica for performing the NGS experiments. The core facility is funded by Academia Sinica Core Facility and Innovative Instrument Project (AS-CFII-108-114). We further thank Dr. Mei-Yeh Lu and Ms. Pei-Lin Chao for their assistance and discussion troubleshooting quality-control metrics. We also thank the staff of the ICOB Marine Research Station for superb help for fish husbandry. Work from our laboratory is supported by a Grand Challenge Grant from Academia Sinica and JSPS KAKENHI grant 22H02678 at OIST. L.B. and D.L. have been supported by a grant from Agence Nationale de la Recherche SENSO (ANR19-CE14-0010).
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