Article (Scientific journals)
Mitochondrial function plasticity in Acanthamoeba castellanii during growth in batch culture.
Czarna, M.; Sluse, Francis; Jarmuszkiewicz, Wieslawa
2007In Journal of Bioenergetics and Biomembranes, 39, p. 149-157
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Keywords :
Acanthamoeba castellanii; alternative oxidase; Cytochrome c oxidase; mitochondrial plasticity; reactive oxygen species; superoxide dismutase; ubiquinone; uncoupling protein
Abstract :
[en] The alterations in mitochondrial bioenergetics during growth in a batch culture of Acanthamoeba castellanii were studied. The capacity of cytochrome pathway-dependent respiration measured in vitro decreased from the intermediary phase, when cell division slowed down. The pattern of the cytochrome pathway capacity changes was paralleled from the intermediary phase by alterations in the amount of total (and reducible) membranous ubiquinone. These changes were accompanied by a decrease in mitochondrial reactive oxygen species production in vitro (when no energy-dissipating system was active), and almost no change in superoxide dismutase activity and protein level, thus indicating an equivalent need for this enzyme in oxidative stress defence in A. castellanii culture. On the other hand, a decrease in the activity and protein level of alternative oxidase and uncoupling protein was observed in vitro, when cells shifted from the exponential growth phase to the stationary phase. It turned out that the contribution of both energy-dissipating systems in the prevention of mitochondrial reactive oxygen species generation in vivo could lead to its constant level throughout the growth cycle of A. castellanii batch culture. Hence, the observed functional plasticity insures survival of high quality cysts of A. castellanii cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Czarna, M.
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Jarmuszkiewicz, Wieslawa
Language :
English
Title :
Mitochondrial function plasticity in Acanthamoeba castellanii during growth in batch culture.
Publication date :
2007
Journal title :
Journal of Bioenergetics and Biomembranes
ISSN :
0145-479X
eISSN :
1573-6881
Publisher :
Kluwer Academic/Plenum Publishers, New York, United States - New York
Volume :
39
Pages :
149-157
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 December 2009

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