Article (Scientific journals)
Proton leak induced by reactive oxygen species produced during in vitro anoxia/reoxygenation in rat skeletal muscle mitochondria.
Navet, Rachel; Mouithys-Mickalad, Ange; Douette, Pierre et al.
2006In Journal of Bioenergetics and Biomembranes, 38, p. 23-32
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Keywords :
mitochondria; Anoxia/reoxygenation; H+ leack; oxphos; uncoupling protein; ROS; Permability transition pore
Abstract :
[en] Superoxide anion generation and the impairment of oxidative phosphorylation yield were studied in rat skeletal muscle mitochondria submitted to anoxia/reoxygenation in vitro. Production of superoxide anion was detected after several cycles of anoxia/reoxygenation. Concomitantly, a decrease of state 3 respiration and phosphorylation yield (ADP/O) were observed. The latter resulted from a proton leak. The presence of palmitic acid during anoxia/reoxygenation cycles led to a dose-dependent inhibition of superoxide anion production together with a partial protection of the ADP/O ratio measured after anoxia/reoxygenation. The ADP/O decrease was shown to be due to a permeability transition pore-sustained proton leak, as it was suppressed by cyclosporine A. The permeability transition pore activation was induced during anoxia/reoxygenation by superoxide anion, as it was cancelled by the spin trap (POBN), which scavenges superoxide anion and by palmitic acid, which induces mitochondrial uncoupling. It can be proposed that the palmitic acid-induced proton leak cancels the production of superoxide anion by mitochondria during anoxia/reoxygenation and therefore prevents the occurrence of the superoxide anion-induced permeability transition pore-mediated proton leak after anoxia/reoxygenation
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Navet, Rachel;  Université de Liège - ULiège
Mouithys-Mickalad, Ange ;  Université de Liège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Douette, Pierre 
Sluse-Goffart, Claudine
Jarmuszkiewicz, Wieslawa
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Language :
English
Title :
Proton leak induced by reactive oxygen species produced during in vitro anoxia/reoxygenation in rat skeletal muscle mitochondria.
Publication date :
2006
Journal title :
Journal of Bioenergetics and Biomembranes
ISSN :
0145-479X
eISSN :
1573-6881
Publisher :
Kluwer Academic/Plenum Publishers, New York, United States - New York
Volume :
38
Pages :
23-32
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 December 2009

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