Plant mitochondria; uncoupling protein activity; inhibition by GTP; Ubiquinone
Abstract :
[en] In isolated potato tuber mitochondria, palmitic acid (PA) can induce a H+ leak inhibited by GTP in the phosphorylating (state 3) respiration but not in the resting (state 4) respiration. The PA-induced H+ leak is constant when state 3 respiration is decreased by an inhibition of the succinate uptake with n-butyl malonate (nBM). We show that the efficiency of inhibition by GTP is decreased when state 3 respiration is progressively inhibited by antimycin A (AA) and is restored following subsequent addition of nBM. We propose that in phosphorylating potato tuber mitochondria, the redox state of ubiquinone, which can antagonistically be varied with AA and nBM, modulates inhibition of the PA-activated UCP-sustained H+ leak by GTP.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Navet, Rachel; Université de Liège - ULiège
Douette, P.; Université de Liège - ULiège
Puttique-Marique, F.
Sluse-Goffart, Claudine; Université de Liège - ULiège
Jarmuszkiewicz, Wieslawa; Université de Liège - ULiège
Sluse, Francis ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Language :
English
Title :
Regulation of uncoupling protein activity in phosphorylating potato tuber mitochondria.
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