Reference : Oxygen consumption and electron spin resonance studies of free radical production by ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Oxygen consumption and electron spin resonance studies of free radical production by alveolar cells exposed to anoxia: inhibiting effects of the antibiotic ceftazidime.
Mouithys-Mickalad, Ange mailto [Université de Liège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Mathy-hartert, M. [> >]
Du, G. [> >]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Deby, C. [> >]
Lamy, M. [> >]
Deby-dupont, G. [> >]
Redox Report : Communications in Free Radical Research
W S Maney and Son
Yes (verified by ORBi)
United Kingdom
[en] Oxygen consumption ; EPR ; Free radical production ; Alveolar cells ; anoxia ; ceftazidime ; inhibitor
[en] By EPR spectroscopy, we investigated free radical production by cultured human alveolar cells subjected to anoxia/re-oxygenation (A/R), and tested the effects of ceftazidime, an antibiotic previously demonstrated to possess antioxidant properties. Two A/R models were performed on type II pneumocytes (A549 cell line), either on cells attached to culture dishes (monolayer A/R model; 3.5 h of anoxia, 30 min of re-oxygenation) or after cell detachment (suspension A/R model; 1 h of anoxia, 10 min of re-oxygenation). Ceftazidime and selective inhibitors (SOD, Tiron, L-NMMA) were added before anoxia. Free radical production was assessed by the EPR spin trapping technique. Oxygen consumption was monitored, in parallel with EPR studies, in the suspension A/R model. The production of free radical species was demonstrated by the generation of PBN-radical adducts: (a(N) = 15.2 G) in the monolayer A/R model and a six-line EPR spectrum (a(N) = 15.7 G and a(H) = 2.7 G) in the suspension A/R model. A kinetic study performed by oximetry, in parallel with EPR spectroscopy, demonstrated marked alterations of the cell respiratory function and that the free radical production started during anoxia and increased during re-oxygenation. In the suspension A/R model, the amplitude of EPR spectra were decreased upon the addition of 200 U/ml SOD (37% inhibition), 0.1 mM Tiron (67% inhibition) and 1 mM L-NMMA (43% inhibition). Addition of 1 mM ceftazidime decreased the amplitude of EPR spectra (37% inhibition) in both A/R models. Complementary in vitro EPR studies demonstrated that CAZ scavenged the hydroxyl radical (produced by the Fenton reaction). The protective effect of ceftazidime in the cell model could thus be linked to its ability to scavenge superoxide anions, nitrogen-derived species and hydroxyl radicals.

File(s) associated to this reference

Fulltext file(s):

Restricted access
publi 231.pdfAuthor postprint7.04 MBRequest copy
Restricted access
publi b231.pdfAuthor postprint771.73 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.