Reference : Respiratory chain network in mitochondria of Candida parapsilosis: ADP/O appraisal of...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Respiratory chain network in mitochondria of Candida parapsilosis: ADP/O appraisal of the multiple electron pathways.
Milani, G. [ > > ]
Jarmuszkiewicz, W. [ > > ]
Sluse-Goffart, C. [ > > ]
Schreiber, A. [ > > ]
Vercesi, A. [ > > ]
Sluse, Francis mailto [Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
FEBS Letters
Elsevier Science
Yes (verified by ORBi)
The Netherlands
[en] Mitochondrion ; electron transport route ; alternative oxidase ; Candida parpsilosis
[en] In this study we demonstrated that mitochondria of Candida parapsilosis contain a constitutive ubiquinol alternative oxidase (AOX) in addition to a classical respiratory chain (CRC) and a parallel respiratory chain (PAR) both terminating by two different cytochrome c oxidases. The C. parapsilosis AOX is characterized by a fungi-type regulation by GMP (as a stimulator) and linoleic acid (as an inhibitor). Inhibitor screening of the respiratory network by the ADP/O ratio and state 3 respiration determinations showed that (i) oxygen can be reduced by the three terminal oxidases through four paths implying one bypass between CRC and PAR and (ii) the sum of CRC, AOX and PAR capacities is higher than the overall respiration (no additivity) and that their engagement could be progressive according to the redox state of ubiquinone, i.e. first cytochrome pathway, then AOX and finally PAR.

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