Diagnosing american bison pregnancy during its two last trimesters: Comparison between rectal palpation associated with ultrasonography and hormonal assays in serum or in feces.
[en] The study aimed to propose sensitive, specific and easy pregnancy diagnosis for American Bison. Trans-rectal Palpation and Ultrasonography (TRPUS), concentration of Pregnancy Associated Glycoproteins (PAG), Progesterone (P4), Estrone (E1), Estrone-Sulfate (E1S) in serum and P4 and E1S in feces were compared. Immunoassay was used for PAG, whereas steroids were assayed using Liquid Chromatography coupled to Mass Spectrometry. Pregnancy and its period were retrospectively assessed after calving. Animals included were distributed as follows: 40 were non-pregnant, 27 were in their second and 15 in their third trimester of gestation. Fisher's test was used to determine sensitivity and specificity of TRPUS. Thresholds leading to best sensitivities and specificities for hormonal assays were determined using Receiver Operating Characteristic Curves. Although TRPUS requires contention, palpation skills, it was conclusive in 70.63 % of cases and good sensitivity (97.50 %) and decent specificity (83.33 %) were observed. Concentrations of PAG, P4, E1S and E1 in serum were higher in Pregnant animals (P), with thresholds giving decent specificity and sensitivity (lower sensitivity for E1 and E1S: 92.11 %; lower specificity for P4: 93.48 %). In feces, P4 and E1S were higher in P: pregnancy diagnosis using E1S assay in feces showed 100 % of sensitivity and specificity. In conclusion, assaying E1S in feces during the two last trimesters of American Bisons pregnancy is a sensitive and specific gestation diagnosis. Pregnancy diagnosis using TRPUS can be useful in ranches but requires contention and skill adaptation. Serum hormonal assays can help evaluating the pregnancy period but also require contention.
Disciplines :
Veterinary medicine & animal health Animal production & animal husbandry Zoology
Author, co-author :
Frisee, Vincent ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Production (DCP) ; Production Animals Department, FARAH Sustainable Livestock Production, Liège University, Belgium
Rigaux, Goulven; Pan Anima, Tournai, Belgium
Dufour, Patrice ; Centre Hospitalier Universitaire de Liège - CHU > > Service de toxicologie clinique, médicolégale, environnementale et en entreprise
Brutinel, Flore ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée ; Theriogenology unit, FARAH Comparative Veterinary Medicine, Liège University, Belgium
Bossaert, Philippe ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Productions animales durables ; Production Animals Department, FARAH Sustainable Livestock Production, Liège University, Belgium
Egyptien, Sophie ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; Theriogenology unit, FARAH Comparative Veterinary Medicine, Liège University, Belgium
Barbato, Olimpia; Veterinary Medicine Department, Perugia University, Perugia, Italy
Farnir, Frédéric ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) ; Biostatistics and bioinformatics applied to Veterinary Sciences, FARAH Sustainable Livestock Production, Liège University, Belgium
Le Goff, Caroline ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Deleuze, Stefan ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée ; Theriogenology unit, FARAH Comparative Veterinary Medicine, Liège University, Belgium
Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Ponthier, Jérôme ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Language :
English
Title :
Diagnosing american bison pregnancy during its two last trimesters: Comparison between rectal palpation associated with ultrasonography and hormonal assays in serum or in feces.
Barbato, O., Menchetti, L., Sousa, N.M., Malfatti, A., Brecchia, G., Canali, C., Beckers, J.F., Barile, V.L., Pregnancy-associated glycoproteins (PAGs) concentrations in water buffaloes (Bubalus bubalis) during gestation and the postpartum period. Theriogenology 97 (2017), 73–77.
Conley, A.J., Review of the reproductive endocrinology of the pregnant and parturient mare. Theriogenology 86 (2016), 355–365.
Conley, A.J., Ball, B.A., Steroids in the establishment and maintenance of pregnancy and at parturition in the mare. Reproduction 158 (2019), R197–R208.
Detilleux, J., Arendt, J., Lomba, F., Leroy, P., Methods for estimating areas under receiver-operating characteristic curves: illustration with somatic-cell scores in subclinical intramammary infections. Prev. Vet. Med. 41 (1999), 75–88.
Dufour, P., Courtois, J., Seynaeve, Y., Peeters, S., Le Goff, C., Cavalier, E., Ponthier, J., Development and validation of a liquid chromatography coupled to mass spectrometer (LC-MS) method for the simultaneous quantification of estrone-3-sulfate, progesterone, estrone and estradiol in serum of mares and American bisons. Res. Vet. Sci. 136 (2021), 343–350.
Dufour, P., Frisee, V., Rigaux, G., Brutinel, F., Egyptien, S., Bossaert, P., Deleersnyder, J., Deleuze, S., Peeters, S., Le Goff, C., Ponthier, J., Cavalier, E., Analysis of progesterone and estrone-sulfate in feces of American Bison using liquid chromatography coupled to mass spectrometry: technical validation and correlation with blood levels. Domest. Anim. Endocrinol., 86, 2024, 106819.
Forgacs, D., Wallen, R.L., Boedeker, A.L., Derr, J.N., Evaluation of fecal samples as a valid source of DNA by comparing paired blood and fecal samples from American bison(Bison bison). BMC Genet, 20, 2019, 22.
Frisee, V., Rigaux, G., Dufour, P., Barbato, O., Brutinel, F., Egyptien, S., Bossaert, P., Deleuze, S., Cavalier, E., Ponthier, J., American Bison (Bison bison) reproductive endocrinology: serum Pregnancy Associated Glycoproteins (PAG), Progesterone, Estrone and Estrone-Sulfate in non pregnant animals and during gestation. Domest. Anim. Endocrinol., 78, 2022, 106684.
Haigh, J.C., Gates, C., Ruder, A., Sasser, R., Diagnosis of pregnancy in wood bison using a bovine assay for pregnancy-specific protein B. Theriogenology 36 (1991), 749–754.
Hollenstein, K., Janett, F., Bleul, U., Hassig, M., Kahn, W., Thun, R., Influence of estradiol on adrenal activity in ovariectomized cows during acute stress. Anim. Reprod. Sci. 93 (2006), 292–302.
Hung, N.N., Prakash, B.S., Influence of gestation on blood plasma concentrations of oestrone and oestrone sulphate in Karan Swiss cows and Murrah buffaloes. Br. Vet. J. 146 (1990), 449–456.
Isobe, N., Nakao, T., Uehara, O., Yamashiro, H., Kubota, H., Plasma concentration of estrone sulfate during pregnancy in different breeds of Japanese beef cattle. J. Reprod. Dev. 49 (2003), 369–374.
Izhar, M., Pasmanik, M., Shemesh, M., Bovine placental progesterone synthesis. Comp. first Second trimesters Gestation. Biol. Reprod. 46 (1992), 846–852.
Kirkpatrick, J.F., Bancroft, K., Kincy, V., Pregnancy and ovulation detection in bison (Bison bison) assessed by means of urinary and fecal steroids. J. Wildl. Dis. 28 (1992), 590–597.
Kirkpatrick, J.F., Kincy, V., Bancroft, K., Shideler, S.E., Lasley, B.L., Oestrous cycle of the North American bison (Bison bison) characterized by urinary pregnanediol-3-glucuronide. J. Reprod. Fertil. 93 (1991), 541–547.
Love, D.M., Mefford, M.T., Ramer, J.C., Validation of the BioPRYN enzyme-linked immunosorbent assay for detection of pregnancy-specific protein-B (PSPB) and diagnosis of pregnancy in American bison (Bison bison). Reprod. Domest. Anim. 52 (2017), 791–797.
McCorkell, R.B., Woodbury, M.R., Adams, G.P., Serial ovarian ultrasonography in wild-caught wood bison (Bison bison athabascae). Theriogenology 80 (2013), 552–556.
McCorkell, R., Wynne-Edwards, K., Galbraith, J., Schaefer, A., Caulkett, N., Boysen, S., Pajor, E., of, U.C., Transport versus on-farm slaughter of bison: physiological stress, animal welfare, and avoidable trim losses. Can. Vet. J. 54 (2013), 769–774.
Ponthier, J., Franck, T., Detilleux, J., Mottart, E., Serteyn, D., Deleuze, S., Association between myeloperoxidase concentration in equine frozen semen and post-thawing parameters. Reprod. Domest. Anim. 45, 811-816, 2010.
Shah, K.D., Nakao, T., Kubota, H., Maeda, T., Peripartum changes in plasma estrone sulfate and estradiol-17beta profiles associated with and without the retention of fetal membranes in holstein-friesian cattle. J. Reprod. Dev. 53 (2007), 279–288.
Shahin, M., Friedrich, M., Gauly, M., Holtz, W., Pregnancy-associated glycoprotein (PAG) profile of Holstein-Friesian cows as compared to dual-purpose and beef cows. Reprod. Domest. Anim. 49 (2014), 618–620.
Shemesh, M., Production and regulation of progesterone in bovine corpus luteum and placenta in mid and late gestation: a personal review. Reprod. Fertil. Dev. 2 (1990), 129–135.
Shemesh, M., Harel-Markowitz, E., Gurevich, M., Shore, L.S., Staurosporine stimulates progesterone production by bovine placental cells. Biol. Reprod. 51 (1994), 146–151.
Singh, S.P., Ramachandran, N., Sharma, N., Goel, A.K., Gururaj, K., Kharche, S.D., Temporal changes in plasma profile of pregnancy-associated glycoprotein, progesterone and estrone sulfate associated with fetal number during early- and mid-pregnancy in goats. Anim. Reprod. Sci. 205 (2019), 115–125.
Tsang, C.P., Changes in plasma levels of estrone sulfate and estrone in the pregnant ewe around parturition. Steroids 23 (1974), 855–868.
Vervaecke, H., Schwarzenberger, F., Endocrine and behavioral observations during transition of non-breeding into breeding season in female American bison (Bison bison). Theriogenology 66 (2006), 1107–1114.
Watson, E.M., Kurth, K.A., Metts, D.L., Moorey, S.E., Miller, B.F., Gerhold, R.W., Muller, L.I., Evaluating the efficacy of noninvasive fecal sampling for pregnancy detection in Elk (Cervus Canadensis). J. Wildl. Dis. 59 (2023), 61–70.
Whitlock, B.K., Coffman, E.A., Coetzee, J.F., Daniel, J.A., Electroejaculation increased vocalization and plasma concentrations of cortisol and progesterone, but not substance P, in beef bulls. Theriogenology 78 (2012), 737–746.
