Article (Scientific journals)
Neuregulin-1 modulates the differentiation of neural stem cells in vitro trough an interaction with the Swi/Snf complex.
Pirotte, Dorothée; Wislet, Sabine; Cloes, Jean-Michel et al.
2010In Molecular and Cellular Neuroscience
Peer Reviewed verified by ORBi
 

Files


Full Text
DP.pdf
Publisher postprint (542.58 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The neuregulin-1 (Nrg-1) gene is translated into several protein isoforms, which are either secreted or membrane-anchored. In vitro, neural stem cells (NSC) express mainly the cystein-rich-domain NRG (CRD-NRG) isoform, a membrane-anchored type III form. This isoform exhibits a cystein-rich-domain, which constitutes a second transmembrane domain and can be cleaved to release both a signaling EGF-containing domain (ECD) at the cell surface and an intracellular domain (ICD). The main goal of this paper was to determine the exact role of ECD and ICD in NSC survival and differentiation. Using an siRNA approach, we demonstrated that CRD-NRG inhibition was followed by a decrease in NSC proliferation and of neuronal or oligodendroglial differentiation. Overexpression of ICD but not ECD was followed by a decrease in NSC proliferation and an increase in neuronal and oligodendroglial differentiation. Moreover, we showed that ICD physically interacted in cultured NSC with BRM and BAF57, two members of the Swi/Snf remodeling complex, and that ICD stimulation of neuronal cell differentiation is dependent on the presence of BAF57.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pirotte, Dorothée ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Wislet, Sabine  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Cloes, Jean-Michel
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
Neuregulin-1 modulates the differentiation of neural stem cells in vitro trough an interaction with the Swi/Snf complex.
Publication date :
2010
Journal title :
Molecular and Cellular Neuroscience
ISSN :
1044-7431
eISSN :
1095-9327
Publisher :
Academic Press
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 December 2009

Statistics


Number of views
261 (13 by ULiège)
Number of downloads
406 (12 by ULiège)

Scopus citations®
 
10
Scopus citations®
without self-citations
10
OpenCitations
 
11

Bibliography


Similar publications



Contact ORBi