Article (Scientific journals)
Variation in the transmission of barley yellow dwarf virus-PAV by different Sitobion avenae clones in China
Yu, Wenjuan; Xu, Zhaohuan; Francis, Frédéric et al.
2013In Journal of Virological Methods, 194 (1-2), p. 1 - 6
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Keywords :
Barley yellow dwarf virus; RT-PCR; Sitobion avenae; TAS-ELISA; Transmission efficiency; Animals; Aphids; Belgium; China; Hordeum; Luteovirus; Plant Diseases; coat protein; Aphididae; article; biological model; coat protein gene; controlled study; gene sequence; genetic analysis; genetic distance; genetic variability; geographical variation (species); molecular cloning; nonhuman; nucleotide sequence; priority journal; sequence alignment; sequence analysis; sequence homology; strain difference; viral phenomena and functions; virus cell interaction; virus gene; virus isolation; virus strain; virus transmission; virus transmission efficiency; virus transmission frequency; wheat
Abstract :
[en] Fourteen Sitobion avenae Fabricius (Hemiptera: Aphididae) clonal lines (clones) originating from China were tested for their ability to transmit BYDV-PAV (one isolate from Belgium and another from China) using wheat plants. By sequence analysis, the coat protein gene of BYDV-PAV-BE was distinguishable from BYDV-PAV-CN. All of the clones could transmit BYDV-PAV, and the transmission varied from 24.42% to 66.67% with BYDV-PAV-BE and from 23.55% to 56.18% with BYDV-PAV-CN. These data suggest that S. avenae has no specialty in BYDV-PAV isolate. Significant differences in the transmission frequencies between the clones with BYDV-PAV-BE and BYDV-PAV-CN were observed. The transmission efficiencies of aphid clones from the middle-lower reaches of Yangtze River (AH, HD, HDE, HZ, JZ, JY and SJ) and Yunnan province (YH) were similar. Nevertheless, differences in the virus transmission efficiencies of the clones from northern (ST and STA) and northwestern (QX, SB and XS) regions were assessed. The transmission efficiency of S. avenae from northern and northwestern China, where BYDV impact is more important, was higher than that from the middle-lower reaches of the Yangtze River and Yunnan province. This work emphasizes the importance of considering aphid vector clonal diversity in addition to virus strain variability when assessing BYDV transmission efficiency. © 2013 Elsevier B.V.
Disciplines :
Entomology & pest control
Author, co-author :
Yu, Wenjuan;  State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China ; Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, B-5030 Gembloux, Passage des Déportés 2, Belgium
Xu, Zhaohuan;  College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Liu, Yong;  College of Plant Protection, Shandong Agricultural University, Taian 271018, China
Cheng, Dengfa;  State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Bragard, Claude;  Earth and Life Institute, Applied Microbiologye-Phytopathology, Université catholique de Louvain, 1348 Louvain-la-Neuve, Croix du Sud 2bte3, Belgium
Chen, Julian;  State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Language :
English
Title :
Variation in the transmission of barley yellow dwarf virus-PAV by different Sitobion avenae clones in China
Publication date :
01 December 2013
Journal title :
Journal of Virological Methods
ISSN :
0166-0934
eISSN :
1879-0984
Publisher :
Elsevier, Nl
Volume :
194
Issue :
1-2
Pages :
1 - 6
Peer reviewed :
Peer Reviewed verified by ORBi
Funding number :
2010DFA32810; 2060302; PIC SHANDONG
Funding text :
AcknowledgementsThis study was supported by the International CooperationProject between China and Belgium (2010DFA32810), and Inter-University Targeted Project between Belgium and China (PICSHANDONG) was funded by the Cooperation universitaire audéveloppement (CUD), and Modern Agro-industry TechnologyResearch System (2060302).
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