Novel insights into the effect of arbuscular mycorrhizal fungi inoculation in soils under long-term biosolids application: emphasis on antibiotic and metal resistance genes, and mobile genetic elements.
IS91 gene; Metagenome; heavy metals; keystone taxa; soil microbial community
Abstract :
[en] The application of biosolids can improve soil fertility and crop productivity but also accompanies risks of heavy metals and antibiotics introduction. In the presence of heavy metals contamination, using arbuscular mycorrhizal fungi (AMF) is a promising strategy to enhance soil microbial community stability and plant tolerance resistance to heavy metals, and to reduce the spread of antibiotic resistance genes (ARGs). The present study investigated the impacts of AMF inoculation on soil and plant heavy metal contents, and soil microbial communities by pot experiments. The results showed that AMF inoculation significantly enhanced plant biomass, and reduced soil and plant heavy metals contents. While AMF inoculation did not alter bacterial and fungal community compositions, it increased bacterial diversity at higher biosolids concentrations. Notably, AMF inoculation enhanced microbial network complexity and increased keystone taxa abundance. Furthermore, several beneficial microorganisms with high resistance to heavy metals were enriched in AMF-inoculated soils. Metagenomic analysis revealed a reduction in the mobile genetic element (MGE) gene IS91 in AMF-inoculated soils and an increase in heavy metal resistance genes compared to soils without AMF. The possibility of reduction in MGE-mediated spread of ARGs is one of the key findings of this study. As a caution, this study also detected enrichment of few ARGs in high biosolids-amended soils with AMF inoculation. Overall, AMF inoculation could be a valuable strategy in agriculture for mitigating the environmental risks associated with biosolids, heavy metals and antibiotic resistance, thereby promoting sustainable soil management and health.
Research Center/Unit :
TERRA Research Centre. Plant Sciences - ULiège
Disciplines :
Environmental sciences & ecology
Author, co-author :
Sun, Tao ; Université de Liège - ULiège > TERRA Research Centre
Delaplace, Pierre ; Université de Liège - ULiège > TERRA Research Centre > Plant Sciences
Li, Guihua; State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
James, Anina; State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Pan, Junting ; State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: panjunting@caas.cn
Zhang, Jianfeng; State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jilin Province, Changchun 130112, China. Electronic address: zhangjianfeng@caas.cn
Language :
English
Title :
Novel insights into the effect of arbuscular mycorrhizal fungi inoculation in soils under long-term biosolids application: emphasis on antibiotic and metal resistance genes, and mobile genetic elements.
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