Article (Scientific journals)
Type II to type I transformation of chronically stimulated goat latissimus dorsi muscle: a histoenzymological, biochemical, bioenergetic, and functional study.
RADERMECKER, Marc; Focant, Bruno; Hautecler, T. et al.
1996In European Surgical Research, 28, p. 80-95
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Keywords :
Latissimus dorsi; Muscle transformation; Mitochondrial oxidases; Myosine; LDH; Chronic stimulation
Abstract :
[en] Five goat latissimus dorsi muscles (LDM) were submitted to a progressive chronic electrostimulation program to reach an integrated understanding of the fast-to-slow transformation process in large mammals. LDM were regularly sampled and followed during a period of 8 months. Each sample was simultaneously assessed for histoenzymological study, myosin and LDH isoforms and bioenergetic capacities [NADH dehydrogenase cytochrome c oxidoreductase (NADH Cyt c OR), succinate dehydrogenase cytochrome c oxidoreductase (Succ Cyt c OR), cytochrome c oxidase (Cyt c Ox) and LDH]. Such muscles were also tested with and without completion of II to I transformation for their mechanical properties in isometric and isotonic strain gauge testing. The conversion of fast-to-slow myosin monitored by heavy chain (HC I) and light chain slow component (LC2s) began a few days after stimulation and was almost 100% after 100 days. The H-LDH isoforms evolved similarly but did not reach 100% conversion after 200 days. The activity of respiratory chain oxidases increased within 36 h but to a variable extent and peaked after 32 days, corresponding to a 75% transformation of myosin compared to initial levels. NADH Cyt c OR, Succ Cyt c OR, and Cyt c Ox, respectively increased 10-, 5- and 5-fold. These activities then significantly decreased before the completion of the myofibrillar transformation and reached a plateau with stable activities that remained 2- to 3-fold higher than the unstimulated LDM. LDH activity sharply decreased until day 62 (5-fold) and then plateaued. Functionally, muscle showed a reduced speed of contraction and moderate reduction in power output but had become fatigue-resistant. This study documents the transformation process in large mammals and suggests the dynamic relation between workload, aerobic-anaerobic metabolism and the contractile myofibrillar system.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
RADERMECKER, Marc ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie cardio-vasculaire
Focant, Bruno ;  Centre Hospitalier Universitaire de Liège - CHU > Laboratoire de biologie cellulaire et tissulaire
Hautecler, T.;  Centre Hospitalier Universitaire de Liège - CHU > Laboratoire de Bioénergétique
Huriaux, Françoise ;  Centre Hospitalier Universitaire de Liège - CHU > Laboratoire de biologie cellulaire et tissulaire
Duyckaert, C.;  Centre Hospitalier Universitaire de Liège - CHU > Laboratoire de Bioénergétique
Chaussende, F.
Reznik, Michel ;  Centre Hospitalier Universitaire de Liège - CHU > Laboratoire de Neuropathologie
Limet, Raymond ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie cardio-vasculaire
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Language :
English
Title :
Type II to type I transformation of chronically stimulated goat latissimus dorsi muscle: a histoenzymological, biochemical, bioenergetic, and functional study.
Publication date :
1996
Journal title :
European Surgical Research
ISSN :
0014-312X
eISSN :
1421-9921
Publisher :
S. Karger
Volume :
28
Pages :
80-95
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 December 2009

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