[en] Southern corn leaf blight (SCLB) caused by Bipolaris maydis is an important foliar disease of maize. In this study, a nematode-symbiotic bacterium Xenorhabdus budapestensis strain C72 was identified with remarkable inhibiting effect on mycelial growth and spore germination of B. maydis. The in vitro assay revealed that C72 cell-free culture media (CFCM) with thermostability exhibited broad-spectrum antifungal activities against other several important plant pathogenic fungi. The early colonization of B. maydis were significantly impaired by CFCM treatment under phytotron condition. This antagonism is likely to be the main contributor to the highly efficient plant protection of 40% (v/v) CFCM treatment against B. maydis, and the relative control effect reached to 59.15% and 77.96% in greenhouse and field experiments, which was comparable to the effect of fungicides. Moreover, we found that extracellular enzymes secreted by symbiotic bacterium may be one of the reasons for the antifungal potential of C72. Beside direct antagonistic effects provided by the bacterium, defense related genes were induced in maize after CFCM treatment. In summary, this study reported the first systematic evaluation of the effect of X. budapestensis C72 in controlling SCLB and exploration of its mode of action, then indicating that entomopathogenic bacteria have the potential to become a new and efficient biological control resource for plant fungal disease management.
Disciplines :
Entomology & pest control
Author, co-author :
Li, Bo; State Key Laboratory for Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China ; Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium
Kong, Lingxiao; Integrated Pest Management Center of Hebei Province, Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding, China
Qiu, Dewen; State Key Laboratory for Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Wang, Shuangchao; State Key Laboratory for Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Biocontrol potential and mode of action of entomopathogenic bacteria Xenorhabdus budapestensis C72 against Bipolaris maydis
NSCF - National Natural Science Foundation of China CAAS - Chinese Academy of Agricultural Sciences
Funding number :
2060302-051; 31901939; 2018120301
Funding text :
This work was financially supported by grants from the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (Grant No. CAAS-2060302-051 ), the National Natural Science Foundation of China (Grant No. 31901939 ) and the Program of Finance Department of Hebei Province in China (Grant No. 2018120301 ).
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