[en] Insect trehalases have been identified as promising new targets for pest control. These key enzymes are involved in trehalose hydrolysis and plays an important role in insect growth and development. In this contribution, plant and microbial compounds, namely validamycin A, amygdalin, and phloridzin, were evaluated for their effect, through trehalase inhibition, on Acyrthosiphon pisum aphid. The latter is part of the Aphididae family, main pests as phytovirus vectors and being very harmful for crops. Validamycin A was confirmed as an excellent trehalase inhibitor with an half maximal inhibitory concentration and inhibitor constant of 2.2 × 10-7 and 5 × 10-8 M, respectively, with a mortality rate of ~80% on a A. pisum population. Unlike validamycin A, the insect lethal efficacy of amygdalin and phloridzin did not correspond to their trehalase inhibition, probably due to their hydrolysis by insect β-glucosidases. Our docking studies showed that none of the three compounds can bind to the trehalase active site, unlike their hydrolyzed counterparts, that is, validoxylamine A, phloretin, and prunasin. Validoxylamine A would be by far the best trehalase binder, followed by phloretin and prunasin.
Disciplines :
Entomology & pest control
Author, co-author :
Neyman, Virgile ; Université de Liège - ULiège > TERRA Research Centre ; Laboratoire de Chimie Physique des Biomolécules, UCPTS, University of Namur, Namur, Belgium ; Namur Institute of Structures Matter (NISM), University of Namur, Namur, Belgium
Quicray, Maude ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs ; Institute of Life Earth and Environment (ILEE), University of Namur, Namur, Belgium
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Michaux, Catherine ; Laboratoire de Chimie Physique des Biomolécules, UCPTS, University of Namur, Namur, Belgium ; Namur Institute of Structures Matter (NISM), University of Namur, Namur, Belgium ; Namur Research, Institute for Life Sciences (NARILIS), University of Namur, Namur, Belgium
Language :
English
Title :
Toxicological, biochemical, and in silico investigations of three trehalase inhibitors for new ways to control aphids.
This study was supported by the Win2Wal Research Program\u2014Exercise 2019 (convention n\u00B01910079). Virgile\u00A0Neyman thanks the Belgian National Fund for Scientific Research (FNRS) for his FRIA PhD Student position. Computational resources have been provided by the Consortium des \u00C9quipements de Calcul Intensif (C\u00C9CI), funded by the Fonds de la Recherche Scientifique de Belgique (F.R.S.-FNRS) under Grant No. 2.5020.11 and by the Walloon Region. Catherine Michaux thanks the National Fund for Scientific Research for her senior Research associate position.
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