[en] Little is known about the insect viruses in wheat sawfly, Dolerus tritici, which is an important agricultural insect feeding on wheat leaves. Here, we used RNA sequencing to identify a novel single positive-strand RNA virus from the larvae of wheat sawfly collected in northern China and then determined its complete genome sequence by rapid amplification of cDNA ends. The complete genome is 9,594 nt in length, including a polyA tail at its 3' terminus, and it is predicted to encode a 326.3-kDa polyprotein. Phylogenetic analysis based on deduced amino acid sequences of the polyprotein revealed that this RNA virus clustered in a clade with deformed wing virus of the genus Iflavirus, family Iflaviridae. The full genome of this RNA virus shows 42.0-50.0% sequence identity with other iflaviruses. Comparisons of amino acid sequences showed that the coat protein of this RNA virus is most similar to that of slow bee paralysis virus, with 33.6% identity, suggesting that this virus is a new member in the genus Iflavirus. Thus, we have tentatively designated it as "Dolerus tritici iflavirus 1" (DtIV1). To our knowledge, this is the first report of an insect virus in wheat sawfly.
Disciplines :
Agriculture & agronomy
Author, co-author :
Guo, Jiashu ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China
Liu, Wenwen ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. liuwenwen01@caas.cn
Chen, Chen; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China
Xu, Zhongtian; Institute of Plant Virology, Ningbo University, Ningbo, China
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Wang, Xifeng; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China
Language :
English
Title :
Complete genome sequence of a novel iflavirus from wheat sawfly (Dolerus tritici).
NSCF - National Natural Science Foundation of China
Funding text :
The authors thank Prof. Yingdang Ren (Henan Academy of Agricultural Sciences) for assistance with collection of insect samples.This research was supported by the National Natural Science Foundation of China (32270173).
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