Antibacterial activity of Cinnamomum verum and Thymus vulgaris essential oils on multidrug-resistant zoonotic bacteria isolated from dogs in southern Benin - 2025
Antibacterial activity of Cinnamomum verum and Thymus vulgaris essential oils on multidrug-resistant zoonotic bacteria isolated from dogs in southern Benin
[en] Antibiotic resistance is a major public health problem. The search for new therapeutic alternatives is becoming urgent. Essential oils are a promising alternative. This study aimed to evaluate the antibacterial activities of essential oils from selected plants on multidrug-resistant zoonotic strains isolated from dogs. Essential oils from dried Thymus vulgaris leaves, Cinnamomum verum bark, and Cuminum cyminum seeds were extracted and tested on five multidrug-resistant Escherichia coli and four S. aureus isolated from dogs in southern Benin. The study showed that Thymus vulgaris essential oil was bacteriostatic, with a Minimum Inhibitory Concentration (MIC) equal to 2.5 μl/ml and a Minimum Bactericidal Concentration (MBC) of 17 μl/ml for E. coli strains and 11.25 μl/ml for S. aureus strains. Regarding
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Yaovi, Ayaovi; Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin
Das, Arpita; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
Behera, Rama; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
Azokpota, Paulin; Laboratory of Food Science and Technology, University of Abomey-Calavi, Jericho-Cotonou, Benin
Farougou, Souaïbou; Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin
Baba-Moussa, Lamine; Laboratory of Biochemistry and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, Faculty of Science and Technology, University of Abomey-Calavi, Benin
Michels, Franck ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Ambatipudi, Kiran; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
Sessou, Philippe ; Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin
Language :
English
Title :
Antibacterial activity of Cinnamomum verum and Thymus vulgaris essential oils on multidrug-resistant zoonotic bacteria isolated from dogs in southern Benin
Mugwaneza D, Rwagasore E, El-Khatib Z, Dukuziyaturemye P, Omolo J, et al. Factors associated with inappropriate use of antibiotics among animal health professionals in selected districts of Rwanda, 2021. J Epidemiol Glob Health 2024;14:265–273.
O’Neill J. Tackling drug-resistant infections globally: final report and recommendations (report). Government of the United Kingdom. 2016.
Caneschi A, Bardhi A, Barbarossa A, Zaghini A. The use of antibiotics and antimicrobial resistance in veterinary medicine, a complex phenomenon: a narrative review. Antibiotics 2023;12:487.
Yaovi AB, Sessou P, Tonouhewa ABN, Hounmanou GYM, Thomson D, et al. Prevalence of antibiotic-resistant bacteria amongst dogs in Africa: a meta-analysis review. Onderstepoort J Vet Res 2022;89:e1–e12.
Usai F, Di Sotto A. Trans-cinnamaldehyde as a novel candidate to overcome bacterial resistance: an overview of in vitro studies. Antibiotics 2023;12:254.
Micucci M, Protti M, Aldini R, Frosini M, Corazza I, et al. Thymus vulgaris L. essential oil solid formulation: chemical profile and spasmolytic and antimicrobial effects. Biomolecules 2020;10:860.
Sessou P, Yaovi BA, Yovo M, Gamedjo J, Dossa F, et al. Phytochemistry and antibacterial activity of plants extracts compared with two commercial antibiotics against E coli responsible for avian colibacillosis in Benin. IJPM 2018;10:168.
Sharifi-Rad J, Dey A, Koirala N, Shaheen S, El Omari N, et al. Cinnamomum species: bridging phytochemistry knowledge, pharmacological properties and toxicological safety for health benefits. Front Pharmacol 2021;12:600139.
Kpatinvoh B, Adjou ES, Dahouenon-Ahoussi E, Konfo TRC, Atrevi B, et al. Efficacité des huiles essentielles de trois plantes aromatiques contre la mycoflore d’altération du niéBé (Vigna unguiculata L., Walp) collecté dans les magasins de vente du Sud-Bénin. J App Bioscience 2017;109:10680–10687.
Mamadou R, Moussa I, Philippe S, Yehouenou B, Agbangnan D, et al. Etude phytochimique, activités antiradicalaire, antibactérienne et antifongique d’extraits de sebastiania chamaelea (L.) Müll. arg. JSOAC 2014;037:10–17.
Kowalczyk A, Przychodna M, Sopata S, Bodalska A, Fecka I. Thymol and thyme essential oil-new insights into selected therapeutic applications. Molecules 2020;25:4125.
Amiri H. Essential oils composition and antioxidant properties of three thymus species. Evid Based Complement Alternat Med 2012;2012:728065.
Nagoor Meeran MF, Javed H, Al Taee H, Azimullah S, Ojha SK. Pharmacological properties and molecular mechanisms of thymol: prospects for Its therapeutic potential and pharmaceutical development. Front Pharmacol 2017;8:380.
Balahbib A, El Omari N, Hachlafi NEL, Lakhdar F, El Menyiy N, et al. Health beneficial and pharmacological properties of p-cymene. Food Chem Toxicol 2021;153:112259.
Ersanli C, Tzora A, Skoufos I, Fotou K, Maloupa E, et al. The assessment of antimicrobial and anti-biofilm activity of essential oils against Staphylococcus aureus strains. Antibiotics 2023;12:384.
Rattanachaikunsopon P, Phumkhachorn P. Assessment of factors influencing antimicrobial activity of carvacrol and cymene against Vibrio cholerae in food. J Biosci Bioeng 2010;110:614–619.
Marinelli L, Fornasari E, Eusepi P, Ciulla M, Genovese S, et al. Carvacrol prodrugs as novel antimicrobial agents. Eur J Med Chem 2019;178:515–529.
Sharifi-Rad M, Varoni EM, Iriti M, Martorell M, Setzer WN, et al. Carvacrol and human health: a comprehensive review. Phytother Res 2018;32:1675–1687.
Imran M, Aslam M, Alsagaby SA, Saeed F, Ahmad I, et al. Therapeutic application of carvacrol: a comprehensive review. Food Sci Nutr 2022;10:3544–3561.
Khan I, Bahuguna A, Shukla S, Aziz F, Chauhan AK, et al. Antimicrobial potential of the food-grade additive carvacrol against uropathogenic E. coli based on membrane depolarization, reactive oxygen species generation, and molecular docking analysis. Microb Pathog 2020;142:104046.
Selvaraj A, Valliammai A, Muthuramalingam P, Priya A, Suba M, et al. Carvacrol targets SarA and CrtM of methicillin-resistant Staphylococcus aureus to mitigate biofilm formation and Staphyloxanthin synthesis: an in vitro and in vivo approach. ACS Omega 2020;5:31100–31114.
Andrade-Ochoa S, Nevárez-Moorillón GV, Sánchez-Torres LE, Villanueva-García M, Sánchez-Ramírez BE, et al. Quantitative structure-activity relationship of molecules constituent of different essential oils with antimycobacterial activity against Mycobacterium tuberculosis and Mycobacterium bovis. BMC Complement Altern Med 2015;15:332.
Kachur K, Suntres Z. The antibacterial properties of phenolic isomers, carvacrol and thymol. Crit Rev Food Sci Nutr 2020;60:3042–3053.
Ahmed HM, Ramadhani AM, Erwa IY, Ishag OAO, Saeed MB. Phytochemical screening, chemical composition and antimicrobial activity of cinnamon verum bark. IRJPAC 2020:36–43.
Vazirian M, Alehabib S, Jamalifar H, Fazeli R, Toosi A, et al. Antimicrobial effect of cinnamon (cinnamomum verum J. presl) bark essential oil in cream-filled cakes and pastries. Res J Pharmacogn 2015;2:11–16.
Zhu R, Liu H, Liu C, Wang L, Ma R, et al. Cinnamaldehyde in diabetes: a review of pharmacology, pharmacokinetics and safety. Pharmacol Res 2017;122:78–89.
Doyle AA, Stephens JC. A review of cinnamaldehyde and its derivatives as antibacterial agents. Fitoterapia 2019;139:104405.
Sharifi A, Mohammadzadeh A, Salehi TZ, Mahmoodi P, Nourian A. Cuminum cyminum L. Essential Oil: a promising antibacterial and antivirulence agent against multidrug-resistant Staphylococcus aureus. Front Microbiol 2021;12:667833.