Article (Scientific journals)
An anionic class III peroxidase from zucchini may regulate hypocotyl elongation through its auxin oxidase activity.
Cosio, Claudia; Vuillemin, Loic; De Meyer, Mireille et al.
2009In Planta
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Keywords :
Auxin oxidase activity; cell elangation; cell wall; pectin; peroxidase
Abstract :
[en] The high number of peroxidase genes explains the description of numerous physiological functions and the fact that the in planta function of a single isoform has never been characterized yet. We analyzed in transgenic Arabidopsis thaliana the localization of a zucchini isoperoxidase (APRX), previously purified thanks to its pectin binding ability. We confirmed that the protein is localized near the cell wall, mainly produced in the elongation area of the hypocotyls and respond to exogenous auxin. In addition, the ectopic overexpression of APRX induced changes in growth pattern and a significant reduction of endogenous indole-3-acetic acid (IAA) level. In agreement with these observations APRX showed an elevated in vitro auxin oxidase activity. We propose that APRX participates in the negative feedback regulation of auxin level and consequently terminates the hypocotyl elongation process.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Biochemistry, biophysics & molecular biology
Author, co-author :
Cosio, Claudia;  Université de Genève - UNIGE > Biochimie et phisiologie végétales
Vuillemin, Loic;  Université de Genève - UNIGE > Biochimie et physiologie végétales
De Meyer, Mireille;  Université de Genève - UNIGE > Biochimie et physiologie végétales
Kevers, Claire ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Penel, Claude;  Université de Genève - UNIGE > Biochimie et physiologie végétales
Dunand, Christophe;  Université de Genève - UNIGE > Biochimie et physiologie végétales
Language :
English
Title :
An anionic class III peroxidase from zucchini may regulate hypocotyl elongation through its auxin oxidase activity.
Publication date :
2009
Journal title :
Planta
ISSN :
0032-0935
eISSN :
1432-2048
Publisher :
Springer Science & Business Media B.V., New York, United States - New York
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 02 January 2009

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