[en] The aim of this investigation was to evaluate the effect of pH variation, sucrose, salt and hydrocolloid gums (xanthan, guar, karaya and locust bean) addition on the gelling properties and the water holding capacity (WHC) of the egg white (EW) gel. Tree levels of pH (4.5, 6.5, 8.0), sucrose/salt (1, 3, 6% w/w) and hydrocolloids (0.01%, 0.10%, 0.20% w/w) were tested in this study. Obtained results showed that mechanical properties and WHC of the EW gel were decreased significantly by pH variation. Addition of salt and sugar improved firmness and elasticity of the EW gel. However, the presence of salt decreased the WHC of the gel. Whereas, in the presence of sugar, WHC was slightly improved. The presence of hydrocolloids in EW gel is associated with the modification of the functional properties of the EW proteins. Xanthan gum decreased hardness and strength and increased WHC of the gel to 97.18% at 0.2%. Guar and karaya gums increased mechanical properties of EW gel at the lowest concentration (0.01%). Locust bean gum was shown to enhance texture profile of EW gel at all tested levels. WHC was significantly improved in the presence of food gums. Microstructure observations of EW gels were also carried out by environmental scanning electron microscopy.
Disciplines :
Food science
Author, co-author :
Khemakhem, Maissa; Ecole Supérieure des Industries Alimentaires de Tunis, Tunisia ; Laboratoire de Chimie Organique Structurale: Synthèse et Etude Physicochimique, Faculté des Sciences de Tunis, Campus Universitaire 2092 - El Manar, Tunisia
Attia, Hamadi; Food Analysis Laboratory, National Engineering School of Sfax (ENIS), Sfax, Tunisia
Ayadi, Mohamed ; Food Analysis Laboratory, National Engineering School of Sfax (ENIS), Sfax, Tunisia
Language :
English
Title :
The effect of pH, sucrose, salt and hydrocolloid gums on the gelling properties and water holding capacity of egg white gel
AFNOR, Produits dérivés des fruits et légumes. 1970, Afnor, Paris NF 05-108.
AFNOR, Céréales, légumineuses et produits dérivés. 1981, Afnor, Paris NF 03-760.
AOAC, Official methods of analysis. 16th ed., 1997, Association of official analytical Chemists, washington, dc.
Ayadi, M.A., Khemakhem, M., Belgith, H., Attia, H., Effect of moderate spray drying conditions on functionality of dried egg white and whole egg. Journal of Food Science 73 (2008), 281–287.
Benichou, A., Aserin, A., Lutz, R., Garti, N., Formation and characterization of amphiphilic conjugates of whey protein isolate (WPI)/xanthan to improve surface activity. Food Hydrocolloids 21:3 (2007), 379–391.
Bertrand, M., Turgeon, S.L., Improved gelling properties of whey protein isolate by addition of xanthan gum. 21, 2007, 159–166.
Campbell, L., Raikos, V., Euston, S.R., Modification of functional properties of egg-white proteins. Nahrung-Food 47:6 (2003), 369–376.
Croguennec, T., Nau, F., Brulé G., Influence of pH and salts on egg white gelation. JFS: Food Engineering and Physical Properties 67:2 (2002), 608–614.
Davis, J.P., Foegeding, E.A., Comparisons of the foaming and interfacial properties of whey protein isolate and egg white proteins. 54, 2007, 200–210.
Doublier, J.-L., Garnier, C., Renard, D., Sanchez, C., Protein–polysaccharide interactions. Current Opinion in Colloid & Interface Science 5:3–4 (2000), 202–214.
Grenha, A., Dionísio, M., Locust bean gum: Exploring its potential for biopharmaceutical applications. Journal of Pharmacy and BioAllied Sciences, 4, 2012, 175.
Hammershoj, M., Physicochemical effects on egg albumen gel texutural properties evaluated by large-deformation studies. Annual Transactions of the Nordic Rheology Society 9 (2001), 107–109.
Handa, A., Takahashi, K., Kuroda, N., Froning, G.W., Heat-induced egg white gels as affected by pH. Journal of Food Science 63:3 (1998), 403–407.
Hao, Y., Wang, F., Huang, W., Tang, X., Zou, Q., Li, Z., et al. Sucrose substitution by polyols in sponge cake and their effects on the foaming and thermal properties of egg protein. Food Hydrocolloids 57 (2016), 153–159.
Horstmann, S.W., Axel, C., Arendt, E.K., Water absorption as a prediction tool for the application of hydrocolloids in potato starch-based bread. Food Hydrocolloids 81 (2018), 129–138.
Ibanoglu, E., Effect of hydrocolloids on the thermal denaturation of proteins. Food Chemistry 90 (2005), 621–626.
Ibanoglu, E., Erçelebi, E.A., Thermal denaturation and functional properties of egg proteins in the presence of hydrocolloid gums. Food Chemistry 101:2 (2007), 626–633.
Imeson, A.P., Ledward, D.A., Mitchell, J.R., On the nature of the interaction between some anionic polysaccharides and proteins. Journal of the Science of Food and Agriculture 28 (1977), 661–668.
Kato, a, Ibrahim, H.R., Watanabe, H., Honma, K., Kobayashi, K., New approach to improve the gelling and surface functional-properties of dried egg-white by heating in dry state. Journal of Agricultural and Food Chemistry 37:2 (1989), 433–437.
Kim, J.H.J., Varankovich, N.V., Nickerson, M.T., The effect of pH on the gelling behaviour of canola and soy protein isolates. Food Research International 81 (2016), 31–38.
Kulmyrzaev, A., Bryant, C., McClements, D.J., Influence of sucrose on the thermal denaturation, gelation, and emulsion stabilization of whey proteins. Journal of Agricultural and Food Chemistry 48:5 (2000), 1593–1597.
Laneuville, S.I., Sanchez, C., Turgeon, S.L., Hardy, J., Paquin, P., Small-angle static light-scattering study of associative phase separation kinetics in β-lactoglobulin + xanthan gum mixtures under shear. Dickinson, E., (eds.) Food Colloids: Interactions, microstructure and processing, 2005, Royal Society of Chemistry, Cambrige, 443–465.
Le, X.T., Turgeon, S.L., Rheological and structural study of electrostatic cross-linked xanthan gum hydrogels induced by β-lactoglobulin. Soft Matter, 9(11), 2013, 3063.
Li, J., Li, X., Wang, C., Zhang, M., Xu, Y., Zhou, B., et al. Characteristics of gelling and water holding properties of hen egg white/yolk gel with NaCl addition. Food Hydrocolloids 77 (2018), 887–893.
Li, J., Wang, C., Zhang, M., Zhai, Y., Zhou, B., Su, Y., et al. Effects of selected phosphate salts on gelling properties and water state of whole egg gel. Food Hydrocolloids 77 (2018), 1–7.
Manzocco, L., Panozzo, A., Nicoli, C.M., Effect of ultraviolet processing on selected properties of egg white. Food Chemistry 135 (2012), 522–527.
Matsudomi, N., Kanda, Y., Moriwaki, H., r-Casein improves the gel properties of dried egg white. Journal of Agricultural and Food Chemistry 51 (2003), 7140–7145.
Matsudomi, N., Takahashi, H., Miyata, T., Some structural properties of ovalbumin heated at 80° C in the dry state. Food Research International 34 (2001), 229–235.
Mekhloufi, G., Sanchez, C., Renard, D., Guillemin, S., Hardy, J., PH-induced structural transitions during complexation and coacervation of β-lactoglobulin and acacia gum. Langmuir 21:1 (2005), 386–394.
Miller, G.L., Use of dinitrosalycilic acid reagent for determination of reducing sugar. Analytical Chemistry 31 (1959), 426–428.
Mine, Y., Recent advances in the understanding of egg white protein functionality. Trends in Food Science & Technolog 6 (1995), 225–232.
Mine, Y., Effect of pH during the dry heating on the gelling properties of egg white proteins. Food Research International 29 (1996), 155–161.
Mohammadi Nafchi, A., Tabatabaei, R.H., Pashania, B., Rajabi, H.Z., Karim, A.A., Effects of ascorbic acid and sugars on solubility, thermal, and mechanical properties of egg white protein gels. International Journal of Biological Macromolecules 62 (2013), 397–404.
Nasabi, M., Labbafi, M., Mousavi, M.E., Madadlou, A., Effect of salts and nonionic surfactants on thermal characteristics of egg white proteins. International Journal of Biological Macromolecules 102 (2017), 970–976.
Noel, T.R., Krzeminski, A., Moffat, J., Parker, R., Wellner, N., Ring, S.G., The deposition and stability of pectin/protein and pectin/poly-l-lysine/protein multilayers. Carbohydrate Polymers 70:4 (2007), 393–405.
Raikos, V., Campbell, L., Stephen, R.E., Rheology and texture of hen ’ s egg protein heat-set gels as affected by pH and the addition of sugar and/or salt. Food Hydrocolloids 21 (2007), 237–244.
Razi, S.M., Motamedzadegan, A., Shahidi, A., Rashidinejad, A., The effect of basil seed gum (BSG) on the rheological and physicochemical properties of heat-induced egg albumin gels. Food Hydrocolloids 82 (2018), 268–277.
Rossi, M., Casiraghi, E., Primavesi, L., Pompei, C., Hidalgo, A., Functional properties of pasteurised liquid whole egg products as affected by the hygienic quality of the raw eggs. Lebensmittel-Wissenschaft und -Technologie- Food Science and Technology 43 (2010), 436–441.
Sadahira, M.S., Lopes, F.C.R., Rodrigues, M.I., Yamada, A.T., Cunha, R.L., Netto, F.M., Effect of pH and interaction between egg white protein and hydroxypropymethylcellulose in bulk aqueous medium on foaming properties. Carbohydrate Polymers 125 (2015), 26–34.
Sadahira, M.S., Rodrigues, M.I., Akhtar, M., Murray, B.S., Netto, F.M., Effect of egg white protein-pectin electrostatic interactions in a high sugar content system on foaming and foam rheological properties. Food Hydrocolloids 58 (2016), 1–10.
Sánchez-gimeno, A.C., Vercet, A., López-buesa, P., Studies of ovalbumin gelation in the presence of carrageenans and after manothermosonication treatments. Innovative Food Science & Emerging Technologies 7 (2006), 270–274.
Souza, C.J.F., Garcia-rojas, E.E., Food Hydrocolloids Interpolymeric complexing between egg white proteins and xanthan gum : Effect of salt and protein/polysaccharide ratio. Food Hydrocolloids 66 (2017), 268–275.
Souza, C.J.F., Souza, C.S.F., Heckert, L.P., Garcia-rojas, E.E., Interpolymer complexation of egg white proteins and carrageenan : Phase behavior, thermodynamics and rheological properties. International Journal of Biological Macromolecules 109 (2018), 467–475.
Stading, M., Langton, M., Hermansson, A.-M., Microstructure and rheological behaviour of particulate β-lactoglobulin gels. Food Hydrocolloids 7:3 (1993), 195–212.
Turgeon, S.L., Schmitt, C., Sanchez, C., Protein–polysaccharide complexes and coacervates. Current Opinion in Colloid & Interface Science 12:4–5 (2007), 166–178.
Van den Berg, M., Jara, F.L., Pilosof, A.M.R., Performance of egg white and hydroxypropylmethylcellulose mixtures on gelation and foaming. Food Hydrocolloids 48 (2015), 282–291.
Wongsasulak, S., Yoovidhya, T., Bhumiratana, S., Hongsprabhas, P., Physical properties of egg albumen and cassava starch composite network formed by a salt-induced gelation method. 40, 2007, 249–256.
Woodward, S.A., Cotterill, O.J., Texture and microstructure of heat-formed egg white gels. Journal of Food Science 51:2 (1986), 333–339.
Zhang, S., Zhang, Z., Vardhanabhuti, B., Effect of charge density of polysaccharides on self-assembled intragastric gelation of whey protein/polysaccharide under simulated gastric conditions. Food & Function 5 (2014), 1829–1838.