[en] Camel α-lactalbumin (Ala-C), the main whey protein of camel milk, was purified by membrane filtration. Surface hydrophobicity as well as interfacial tension were examined at different levels of pH (3.0, 6.0, 9.0) and protein concentration (0.1 %, 0.2 %, 0.4 % w/w), and compared to bovine α-lactalbumin (Ala-B). The emulsifying properties (EAI and ESI) of oil-in-water emulsions (20 %/80 %) were investigated for both proteins. The stability of the processed emulsions was characterised by ζ-potential, particle size and viscosity measurements. The main findings indicate that Ala-C exhibited greater surface hydrophobicity and undergone changes in conformational structure when pH decreased from 9.0-3.0. These changes were enhanced by increasing protein concentration from 0.1 % to 0.4 % (w/w). However, high concentrations showed low emulsifying activity, especially at pH 6.0 where interfacial tension was lower. In comparison with Ala-B, maximum EAI was close, despite the lower surface hydrophobicity of Ala-C under similar conditions. Overall, emulsions were more viscous at pH 3.0 due to the greater surface coverage than at 9.0 and 6.0. Under the conditions of this study, a protein concentration of 0.2 % resulted in the finest oil droplets and highest viscosity for both types of α-lactalbumin, and Ala-C conferred the highest long-term stability to the emulsions.
Disciplines :
Food science
Author, co-author :
Ellouze, Maroua; Université Clermont Auvergne, CNRS, Sigma Clermont, Institut Pascal. F-63000, Clermont-Ferrand, France, Université de Sfax, Laboratoire d'Analyse, Valorisation et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, Sfax 3038, Tunisia. Electronic address: maroua.ellouze@etu.uca.fr.
Lajnaf, Roua; Université de Sfax, Laboratoire d'Analyse, Valorisation et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, Sfax 3038, Tunisia.
Zouari, Ahmed; Université de Sfax, Laboratoire d'Analyse, Valorisation et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, Sfax 3038, Tunisia.
Attia, Hamadi; Université de Sfax, Laboratoire d'Analyse, Valorisation et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, Sfax 3038, Tunisia.
Ayadi, Mohamed ; Université de Sfax, Laboratoire d'Analyse, Valorisation et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, Sfax 3038, Tunisia.
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