Optimization of ultrasound-assisted extraction of bioactive compounds from Carthamus caeruleus L. rhizome: Integrating central composite design, Gaussian process regression, and multi-objective Grey Wolf optimization approaches
Phenolic extraction efficiency Design of experiments, Predictive modeling Process optimization strategies Metaheuristic optimization Ultrasound assistance
Abstract :
[en] The prediction of ultrasound-assisted extraction (UAE) for total phenolic content (TPC) and total flavonoid content (TFC) from Carthamus caeruleus L. rhizomes was conducted using a Gaussian process regression model (GPR) with a multi-objective Grey Wolf optimization approach (MOGWO). A central composite design (CCD) was employed first, examining ethanol concentration, temperature, time, and solvent-to-solid ratio as independent variables. TPC and TFC responses were analyzed under various conditions, revealing significant quadratic and interaction effects (p < 0.05). The GPR was then utilized to predict TPC and TFC, showing high accuracy with correlation coefficients near 1 and minimal root mean square error (RMSE) values. To simultaneously maximize TPC and TFC, the MOGWO was used in a multi-objective framework. Validation through CCD and GPR highlighted GPR's superior predictive accuracy. Optimal conditions (10 % ethanol, 40°C, 20 minutes sonication, and 50 mL g−1 solvent to solid ratio) showed significant discrepancies in CCD predictions but high accuracy in GPR predictions. An interactive tool predicts TPC and TFC using CCD and GPR models. Users input extraction parameters and receive predictions, with a GWO-based optimization module for optimal conditions. The interface enables model comparison, improves process understanding, and optimizes bioactive compound extraction.
Disciplines :
Biotechnology
Author, co-author :
Moussa, Hamza
Dahmoune, Farid
Lekmine, Sabrina
Mameri, Amal
Tahraoui, Hichem
Hamid, Sarah
Benzitoune, Nourelimane
Moula, Nassim ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Méthodes expérimentales des animaux de laboratoire et éthique en expérimentation animale
Zhang, Jie
Amrane, Abdeltif
Language :
English
Title :
Optimization of ultrasound-assisted extraction of bioactive compounds from Carthamus caeruleus L. rhizome: Integrating central composite design, Gaussian process regression, and multi-objective Grey Wolf optimization approaches
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