gingival tissue; guided bone regeneration; neovascularization; recombinant human bone morphogenic protein 2; vascular endothelial growth factor; Ecology, Evolution, Behavior and Systematics; Biochemistry, Genetics and Molecular Biology (all); Space and Planetary Science; Paleontology
Abstract :
[en] [en] UNLABELLED: The aim of this study is to evaluate the effect on the initiation of new blood vessel formation of rh-BMP-2 administration in the human gingival tissue during bone regeneration surgery.
MATERIAL AND METHODS: The randomized controlled clinical trial included twenty patients with bilateral partial edentulous of the mandibular premolar and molar region. Each patient received one implants on each side. Only one side received a 0.25 µg injection of rhBMP-2 into the gingival flap and grafted material during guided bone regeneration (GBR) for dental implantation. And the other side received GBR without injection. Three samples were collected from each patient as follows: one from the anterior area of the mandible (control group #1) collected at the time of all implant surgeries, and the two other samples during the placement of healing abutments at 4 months of follow-up, from treated side with rh-BMP-2 (test group) and untreated ones (control group #2). A total of 60 gingival samples were collected. Samples were stained with hematoxylin-eosin, and immunohistochemistry was performed with a vascular endothelial growth factor marker. The number of new vessels in each sample was counted.
RESULT: Statistical analyses showed a significantly higher number of new vessels in the gingival tissue of the test group.
CONCLUSIONS: Rh-BMP-2 injections into the gingival flap significantly improved new blood vessel formation.
Disciplines :
Dentistry & oral medicine
Author, co-author :
Chantiri, Mansour; Department of Periodontology, Faculty of Dental Medicine, Lebanese University, Beirut 27798, Lebanon
Namour, Samir ✱; Université de Liège - ULiège > Département des sciences dentaires
El Toum, Sami; Department of Oral Medicine and Maxillofacial Radiology, Faculty of Dental Medicine, Lebanese University, Beirut 27798, Lebanon
Zeinoun, Toni; Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Lebanese University, Beirut 27798, Lebanon
✱ These authors have contributed equally to this work.
Language :
English
Title :
Effect of rh-BMP-2 in the Initiation of Neovascularization in Human Gingival Tissue: A Split-Mouth Clinical Study.
Publication date :
04 December 2023
Journal title :
Life (Basel, Switzerland)
ISSN :
2075-1729
eISSN :
2075-1729
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Anusuya G.S. Kandasamy M. Jacob Raja S.A. Sabarinathan S. Ravishankar P. Kandhasamy B. Bone morphogenetic proteins: Signaling periodontal bone regeneration and repair J. Pharm. Bioallied Sci. 2016 8 (Suppl. S1) S39 S41 10.4103/0975-7406.191964 27829744
Ahmad P. Della Bella E. Stoddart M.J. Applications of Bone Morphogenetic Proteins in Dentistry: A Bibliometric Analysis BioMed Res. Int. 2020 24 5971268 10.1155/2020/5971268 33163536
Aspenberg P. Wang E. Thorngren K.G. Bone morphogenetic protein induces bone in the squirrel monkey, but bone matrix does not Acta. Orthop. Scand. 1992 63 619 622 10.3109/17453679209169721 1471508
Thies R.S. Bauduy M. Ashton B.A. Kurtzberg L. Wozney J.M. Rosen V. Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells Endocrinology 1992 130 1318 1324 10.1210/endo.130.3.1311236 1311236
Gerdes J. Lemke H. Baisch H. Wacker H.H. Schwab U. Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67 J. Immunol. 1984 133 1710 1715 10.4049/jimmunol.133.4.1710 6206131
Kill I.R. Localization of the Ki-67 antigen within the nucleolus: Evidence for a fibrillarin-deficient region of the dense fibrillar component J. Cell Sci. 1996 109 Pt 6 1253 1263 10.1242/jcs.109.6.1253
Folkman J. Haudenschild C. Angiogenesis in vitro Nature 1980 288 551 556 10.1038/288551a0
Xie H. Wang Z. Zhang L. Lei Q. Zhao A. Wang H. Li Q. Chen Z. Zhang W. Development of an angiogenesis-promoting microvesicle-alginate-polycaprolactone composite graft for bone tissue engineering applications PeerJ 2016 4 e2040 10.7717/peerj.2040
Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease Nat. Med. 1995 1 27 31 10.1038/nm0195-27
Ishikawa F. Miyazono K. Hellman U. Drexler H. Wernstedt C. Hagiwara K. Usuki K. Takaku F. Risau W. Heldin C.H. Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial cell growth factor Nature 1989 338 557 562 10.1038/338557a0 2467210
Tischer E. Gospodarowicz D. Mitchell R. Silva M. Schilling J. Lau K. Crisp T. Fiddes J.C. Abraham J.A. Vascular endothelial growth factor: A new member of the platelet-derived growth factor gene family Biochem. Biophys. Res. Commun. 1989 165 1198 1206 10.1016/0006-291X(89)92729-0 2610687
Johnstone S. Logan R.M. Expression of vascular endothelial growth factor (VEGF) in normal oral mucosa, oral dysplasia and oral squamous cell carcinoma Int. J. Oral Maxillofac. Surg. 2007 36 263 266 10.1016/j.ijom.2006.09.017 17141472
Hoeben A. Bart L. Highley M.S. Wildiers H. Van Oosterom A.T. De Bruijn E.A. Vascular endothelial growth factor and angiogenesis Pharmacol. Rev. 2004 56 549 580 10.1124/pr.56.4.3 15602010
Zaid K.W. Nhar B.M. Ghadeer Alanazi S.M. Murad R. Domani A. Alhafi A.J. Lack of Effects of Recombinant Human Bone Morphogenetic Protein2 on Angiogenesis in Oral Squamous Cell Carcinoma Induced in the Syrian Hamster Cheek Pouch Asian Pac. J. Cancer. Prev. 2016 17 3527 3531 10.7314/APJCP.2016.17.3.927 27510004
Radović K. Brković B. Roganović J. Ilić J. Lemić A.M. Jovanović B. Salivary VEGF and post-extraction wound healing in type 2 diabetic immediate denture wearers Acta Odontol. Scand. 2022 80 9 14 10.1080/00016357.2021.1930149
Langenfeld E.M. Langenfeld J. Bone morphogenetic protein-2 stimulates angiogenesis in developing tumors Mol. Cancer Res. 2004 2 141 149 10.1158/1541-7786.141.2.3
Deckers M.M.L. van Bezooijen R.L. van der Horst G. Hoogendam J. van Der Bent C. Papapoulos S.E. Lowik C.W.S.M. Bone morphogenetic proteins stimulate angiogenesis through osteoblast-derived vascular endothelial growth factor A Endocrinology 2002 143 1545 1553 10.1210/endo.143.4.8719
Kerbel R.S. Tumor angiogenesis: Past, present and the near future Carcinogenesis 2000 21 505 515 10.1093/carcin/21.3.505
Koerner J.D. Markova D.Z. Schroeder G.D. Calio B.P. Shah A. Brooks C.W. Vaccaro A.R. Anderson D.G. Kepler C.K. The local cytokine and growth factor response to recombinant human bone morphogenetic protein-2 (rhBMP-2) after spinal fusion Spine J. 2018 18 1424 1433 10.1016/j.spinee.2018.03.006
de Freitas R.M. Spin-Neto R. Marcantonio Junior E. Pereira L.A.V.D. Wikesjö U.M.E. Susin C. Alveolar ridge and maxillary sinus augmentation using rhBMP-2: A systematic review Clin. Implant. Dent. Relat. Res. 2015 17 (Suppl. S1) e192 e205 10.1111/cid.12156
Jung Y.H. Park J.Y. Kim H.J. Lee S.M. Kim S.H. Yun J.H. Regenerative Potential of Bone Morphogenetic Protein 7-Engineered Mesenchymal Stem Cells in Ligature-Induced Periodontitis Tissue Eng. Part A 2023 29 200 210 10.1089/ten.tea.2022.0162 36565024
James A.W. LaChaud G. Shen J. Asatrian G. Nguyen V. Zhang X. Ting K. Soo C. A Review of the Clinical Side Effects of Bone Morphogenetic Protein-2 Tissue Eng. Part B Rev. 2016 22 284 297 10.1089/ten.teb.2015.0357 26857241
Scalzone A. Flores-Mir C. Carozza D.F. Apuzzo F. Grassia V. Perillo L. Secondary alveolar bone grafting using autologous versus alloplastic material in the treatment of cleft lip and palate patients: Systematic and meta-analysis Prog. Orthod. 2019 20 6 10.1186/s40510-018-0252-y 30740615
Kalay E. Ermutlu C. Erekan-Yenigül A. Yalçınkaya U. Sarısözen B. Effect of bone morphogenic protein-2 and desferrioxamine on distraction osteogenesis Injury 2022 53 1854 1857 10.1016/j.injury.2022.03.064 35410738
Pulkkinen H.H. Kiema M. Lappalainen J.P. Toropainen A. Beter M. Tirronen A. Holappa L. Niskanen H. Kaikkonen M.U. Ylä-Herttuala S. et al. BMP6/TAZ-Hippo signaling modulates angiogenesis and endothelial cell response to VEGF Angiogenesis 2021 24 129 144 10.1007/s10456-020-09748-4 33021694
Zhang R. Liu Y. Qi Y. Zhao Y. Nie G. Wang X. Zheng S. Self-assembled peptide hydrogel scaffolds with VEGF and BMP-2 enhanced in vitro angiogenesis and osteogenesis Oral Dis. 2022 28 723 733 10.1111/odi.13785
Liu Z. Xu Z. Wang X. Zhang Y. Wu Y. Jiang D. Jia R. Preparation and Biocompatibility of Core-Shell Microspheres for Sequential, Sustained Release of BMP-2 and VEGF BioMed Res. Int. 2022 25 4072975 10.1155/2022/4072975
Geng Y. Duan H. Xu L. Witman N. Yan B. Yu Z. Wang H. Tan Y. Lin L. Li D. et al. BMP-2 and VEGF-A modRNAs in collagen scaffold synergistically drives bone repair through osteogenic and angiogenic pathways Commun. Biol. 2021 4 82 10.1038/s42003-020-01606-9
Zhang W. Zhu C. Wu Y. Ye D. Wang S. Zou D. Zhang X. Kaplan D.L. Jiang X. VEGF and BMP-2 promote bone regeneration by facilitating bone marrow stem cell homing and differentiation Eur. Cells Mater. 2014 27 1 12 10.22203/eCM.v027a01
Samee M. Kasugai S. Kondo H. Ohya K. Shimokawa H. Kuroda S. Bone Morphogenetic Protein-2 (BMP-2) and Vascular Endothelial Growth Factor (VEGF) Transfection to Human Periosteal Cells Enhance Osteoblast Differentiation and Bone Formation J. Pharmacol. Sci. 2008 108 18 31 10.1254/jphs.08036FP 18776714
Lowery J.W. de Caestecker M.P. BMP signaling in vascular development and disease Cytokine Growth Factor Rev. 2010 21 287 298 10.1016/j.cytogfr.2010.06.001 20674464
David L. Feige J.J. Bailly S. Emerging role of bone morphogenetic proteins in angiogenesis Cytokine Growth Factor Rev. 2009 20 203 212 10.1016/j.cytogfr.2009.05.001 19502096
