[en] In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to the recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against a range of bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that BDI-IS1 produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin and bacilysin, together with some some ribosomally-synthesized and post-translationally modified peptides (RiPPs) such as plantazolicin, and potentially amylocyclicin, bacinapeptin and LCI. Reverse genetics further showed the specific involvement of some of these compounds in the antagonistic activity of the strain. Comparative genomics between the five already sequenced B. nakamurai strains showed that non-ribosomal products constitute the core metabolome of the species while RiPPs are more strain-specific. Although the secondary metabolome lacks some key bioactive metabolites found in B. velezensis, greenhouse experiments show that B. nakamurai BDI-IS1 is able to protect tomato and maize plants against early blight and northern leaf blight caused by Alternaria solani and Exserohilum turcicum, respectively, at levels similar to or better than B. velezensis QST713. The reduction of these foliar diseases, following root or leaf application of the bacterial suspension demonstrates that BDI-IS1 can act by direct antibiosis and by inducing plant defence mechanisms. These findings indicate that B. nakamurai BDI-IS1 can be considered as a good candidate for biocontrol of plant diseases prevailing in tropical regions, and encourage further research into its spectrum of activity, its requirements and the conditions needed to ensure its efficacy.
Nihorimbere, Gaspard ✱; Earth and Life Institute-Applied Microbiology, Université Catholique de Louvain, Croix du Sud 2, Louvain-la-Neuve 1348, Belgium, Research department, Institut des Sciences Agronomiques du Burundi (ISABU), Boulevard du Japon, Rohero 1, P.O Box 795, Bujumbura, Burundi. Electronic address: gaspard.nihorimbere@uclouvain.be
Arias, Anthony Argüelles; Microbial Processes and Interactions (MiPI), Teaching and Research Centre (TERRA), Gembloux Agro-BioTech, University of Liège, Avenue de la Faculté 2B, Gembloux 5030, Belgium. Electronic address: arguellesariasa@gmail.com
Liénard, Charlotte; Earth and Life Institute-Applied Microbiology, Université Catholique de Louvain, Croix du Sud 2, Louvain-la-Neuve 1348, Belgium. Electronic address: lienard.charlotte@hotmail.be
Steels, Sébastien ; Université de Liège - ULiège > Département GxABT > Microbial technologies
Nibasumba, Anaclet; Institut Supérieur de Formation Agricole, Université du Burundi, P.O Box 241, Gitega, Burundi. Electronic address: anaclet.nibasumba@hotmail.fr
Nihorimbere, Venant; Laboratoire de Microbiologie, Faculté d'Agronomie et de BioIngéniérie (FABI), Université du Burundi, Avenue de l'Unesco 2, P.O Box 2940, Bujumbura, Burundi. Electronic address: venant.nihorimbere@gmail.com
Legrève, Anne ✱; Earth and Life Institute-Applied Microbiology, Université Catholique de Louvain, Croix du Sud 2, Louvain-la-Neuve 1348, Belgium. Electronic address: anne.legreve@uclouvain.be
Ongena, Marc ✱; Université de Liège - ULiège > Département GxABT
✱ These authors have contributed equally to this work.
Language :
English
Title :
Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai.
ARES - Académie de Recherche et d'Enseignement Supérieur
Funding text :
The authors thank Papa Makhona Niang and Vincent Bremhorst (Statistical Methodology and Computing Service, technological platform at UCLouvain \u2013 SMCS/LIDAM, UCLouvain) for their support in genome sequence assembly and statistical analyses, respectively. This research was supported by the Acad\u00E9mie de Recherche et d'Enseignement Sup\u00E9rieur-Commission de Coop\u00E9ration au D\u00E9veloppement (ARES-CDD), F\u00E9d\u00E9ration Wallonie-Bruxelles) through the PRD instrument (Title: Vers une agriculture plus performante et durable au Burundi: application de microorganismes pour am\u00E9liorer la sant\u00E9 et la croissance des plantes). MO is Research Director at the FRS-FNRS (National Fund for Scientific Research) in Belgium.The authors thank Papa Makhona Niang and Vincent Bremhorst (Statistical Methodology and Computing Service, technological platform at UCLouvain \u2013 SMCS/LIDAM, UCLouvain) for their support in genome sequence assembly and statistical analyses, respectively. This research was supported by the Acad\u00E9mie de Recherche et d\u2019Enseignement Sup\u00E9rieur-Commission de Coop\u00E9ration au D\u00E9veloppement (ARES-CDD), F\u00E9d\u00E9ration Wallonie-Bruxelles) through the PRD instrument (Title: Vers une agriculture plus performante et durable au Burundi: application de microorganismes pour am\u00E9liorer la sant\u00E9 et la croissance des plantes). MO is Research Director at the FRS-FNRS (National Fund for Scientific Research) in Belgium.
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