[en] Varicella-zoster virus (VZV) encephalitis and meningitis are potential central nervous system (CNS) complications following primary VZV infection or reactivation. With Type-I interferon (IFN) signalling being an important first line cellular defence mechanism against VZV infection by the peripheral tissues, we here investigated the triggering of innate immune responses in a human neurallike environment. For this, we established and characterised 5-month matured hiPSC-derived neurospheroids (NSPHs) containing neurons and astrocytes. Subsequently, NSPHs were infected with reporter strains of VZV (VZV eGFP-ORF23) or Sendai virus (SeV eGFP), with the latter serving as an immune-activating positive control. Live cell and immunocytochemical analyses demonstrated VZV eGFP-ORF23 infection throughout the NSPHs, while SeV eGFP infection was limited to the outer NSPH border. Next, NanoString digital transcriptomics revealed that SeV eGFP-infected NSPHs activated a clear Type-I IFN response, Frontiers in Immunology frontiersin.org 01
Disciplines :
Immunology & infectious disease
Author, co-author :
Govaerts, Jonas; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium ; Antwerp Center for Translational Immunology and Virology (ACTIV), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
Van Breedam, Elise; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
De Beuckeleer, Sarah; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Laboratory of Cell Biology and Histology, Antwerp Center for Advanced Microscopy, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium ; µNEURO Research Centre of Excellence, University of Antwerp, Wilrijk, Belgium
Goethals, Charlotte; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
Peter D'incal, Claudio; Cell Death Signaling -Epigenetics Lab, Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
Di Stefano, Julia; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
Van Calster, Siebe; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
Buyle-Huybrecht, Tamariche; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium ; Antwerp Center for Translational Immunology and Virology (ACTIV), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
Boeren, Marlies; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium ; Antwerp Center for Translational Immunology and Virology (ACTIV), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
De Reu, Hans; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Flow Cytometry and Cell Sorting Core Facility (FACSUA), University of Antwerp, Antwerp, Belgium
Paludan, Søren; Department of Biomedicine, Aarhus University, Aarhus, Denmark
Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Service collectif des enseignements de biologie en bachelier
Lebrun, Marielle ; Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Sadzot, Catherine ; Université de Liège - ULiège > Département des sciences de la vie
Motaln, Helena; Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
Rogelj, Boris; Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia ; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia
Van Weyenbergh, Johan; Laboratory of Clinical and Epidemiological Virology, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium
De Vos, Winnok; Laboratory of Cell Biology and Histology, Antwerp Center for Advanced Microscopy, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium ; µNEURO Research Centre of Excellence, University of Antwerp, Wilrijk, Belgium
Vanden Berghe, Wim; Cell Death Signaling -Epigenetics Lab, Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
Ogunjimi, Benson; Antwerp Center for Translational Immunology and Virology (ACTIV), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Antwerp Unit for Data Analysis and Computation in Immunology and Sequencing (AUDACIS), Antwerp, Belgium ; Centre for Health Economics Research and Modelling Infectious Diseases (CHERMID), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Department of Paediatrics, Antwerp University Hospital, Antwerp, Belgium ; University of Antwerp, Antwerp, Belgium
Delputte, Peter; Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium
Ponsaerts, Peter; Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium ; Flow Cytometry and Cell Sorting Core Facility (FACSUA), University of Antwerp, Antwerp, Belgium
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