Variegation and silencing in a lentiviral-based murine transgenic model.

[en] Lentiviral based constructs represent a recent development in the generation of transgenic animals. The ease of use, and the fact that the same backbone vectors can be used to down-modulate endogenous gene expression and to produce transgenic animals overexpressing a gene of interest, have fuelled growing interest in this technology. In this study, we have used a lentiviral delivery system to generate transgenic mice expressing altered levels (up or downregulated) of a gene of interest. Although this lentiviral-based approach led to high levels of transgenesis and germ line transmission, a wide variation in transgene expression was observed in most first and second generation mouse lines. In particular, despite the segregation of integrants into single-copy expressing mouse lines, transgene expression appeared to be the target of epigenetic regulatory mechanism, often causing the coexistence of high and low transgene expressing cells within a given tissue such as blood peripheral lymphocytes. The establishment and analysis of large number of mouse lines may therefore be required to select a stable transgenic line with pancellular expression of a gene of interest using this lentiviral-based approach.

Disciplines :

Biochemistry, biophysics & molecular biology
Genetics & genetic processes

Author, co-author :

Baup, Delphine; Université Libre de Bruxelles - ULB

Fraga, Laurent; Université Libre de Bruxelles - ULB

Pernot, Eileen; Université Libre de Bruxelles - ULB

Van Acker, Annette; Université Libre de Bruxelles - ULB

Vanherck, Anne-Sophie; Université Libre de Bruxelles - ULB

Breckpot, Karine; School of the Vrije Universiteit Brussel

Thielemans, Kris; School of the Vrije Universiteit Brussel

Schurmans, Stéphane ; Université Libre de Bruxelles - ULB

Moser, Muriel

Leo, Oberdan; Université Libre de Bruxelles - ULB

Language :

English

Title :

Variegation and silencing in a lentiviral-based murine transgenic model.

Publication date :

2010

Journal title :

Transgenic Research

ISSN :

0962-8819

eISSN :

1573-9368

Publisher :

Kluwer Academic Publishers, Dordrecht, Netherlands

Volume :

Pages :

399-414

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 10 December 2009

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