Article (Scientific journals)
Variegation and silencing in a lentiviral-based murine transgenic model.
Baup, Delphine; Fraga, Laurent; Pernot, Eileen et al.
2010In Transgenic Research, 19, p. 399-414
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Keywords :
Lentiviral transgenesis; Silencing; Variegation; RNA interference; Mice
Abstract :
[en] Lentiviral based constructs represent a recent development in the generation of transgenic animals. The ease of use, and the fact that the same backbone vectors can be used to down-modulate endogenous gene expression and to produce transgenic animals overexpressing a gene of interest, have fuelled growing interest in this technology. In this study, we have used a lentiviral delivery system to generate transgenic mice expressing altered levels (up or downregulated) of a gene of interest. Although this lentiviral-based approach led to high levels of transgenesis and germ line transmission, a wide variation in transgene expression was observed in most first and second generation mouse lines. In particular, despite the segregation of integrants into single-copy expressing mouse lines, transgene expression appeared to be the target of epigenetic regulatory mechanism, often causing the coexistence of high and low transgene expressing cells within a given tissue such as blood peripheral lymphocytes. The establishment and analysis of large number of mouse lines may therefore be required to select a stable transgenic line with pancellular expression of a gene of interest using this lentiviral-based approach.
Disciplines :
Biochemistry, biophysics & molecular biology
Genetics & genetic processes
Author, co-author :
Baup, Delphine;  Université Libre de Bruxelles - ULB
Fraga, Laurent;  Université Libre de Bruxelles - ULB
Pernot, Eileen;  Université Libre de Bruxelles - ULB
Van Acker, Annette;  Université Libre de Bruxelles - ULB
Vanherck, Anne-Sophie;  Université Libre de Bruxelles - ULB
Breckpot, Karine;  School of the Vrije Universiteit Brussel
Thielemans, Kris;  School of the Vrije Universiteit Brussel
Schurmans, Stéphane  ;  Université Libre de Bruxelles - ULB
Moser, Muriel
Leo, Oberdan;  Université Libre de Bruxelles - ULB
Language :
English
Title :
Variegation and silencing in a lentiviral-based murine transgenic model.
Publication date :
2010
Journal title :
Transgenic Research
ISSN :
0962-8819
eISSN :
1573-9368
Publisher :
Kluwer Academic Publishers, Dordrecht, Netherlands
Volume :
19
Pages :
399-414
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 December 2009

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