Calsenilin is required for endocrine pancreas development in zebrafish.

Animals; Animals, Genetically Modified; Embryo, Nonmammalian/embryology/metabolism; Endocrine System/embryology/metabolism; Gene Expression Regulation, Developmental; Kv Channel-Interacting Proteins/genetics/metabolism; Mutation/genetics; Pancreas/embryology/metabolism; Pancreatic Hormones/metabolism; Receptors, Notch/metabolism; Signal Transduction; Tretinoin/metabolism; Zebrafish/embryology/genetics/metabolism

Abstract :

[en] Calsenilin/DREAM/Kchip3 is a neuronal calcium-binding protein. It is a multifunctional protein, mainly expressed in neural tissues and implicated in regulation of presenilin processing, repression of transcription, and modulation of A-type potassium channels. Here, we performed a search for new genes expressed during pancreatic development and have studied the spatiotemporal expression pattern and possible role of calsenilin in pancreatic development in zebrafish. We detected calsenilin transcripts in the pancreas from 21 somites to 39 hours postfertilization stages. Using double in situ hybridization, we found that the calsenilin gene was expressed in pancreatic endocrine cells. Loss-of-function experiments with anti-calsenilin morpholinos demonstrated that injected morphants have a significant decrease in the number of pancreatic endocrine cells. Furthermore, the knockdown of calsenilin leads to perturbation in islet morphogenesis, suggesting that calsenilin is required for early islet cell migration. Taken together, our results show that zebrafish calsenilin is involved in endocrine cell differentiation and morphogenesis within the pancreas.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Stetsyuk, V.

Peers, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Mavropoulos, A.

Verbruggen, Vincianne ; Université de Liège - ULiège > GIGA-Research

Thisse, B.

Thisse, C.

Motte, Patrick ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Duvillie, B.

Scharfmann, R.

Language :

English

Title :

Calsenilin is required for endocrine pancreas development in zebrafish.

Publication date :

2007

Journal title :

Developmental Dynamics

ISSN :

1058-8388

eISSN :

1097-0177

Publisher :

Wiley Liss, Inc., New York, United States - New York

Volume :

236

Issue :

Pages :

1517-25

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 10 December 2009

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