Animals; Cattle/genetics; Phenotype; Mite Infestations/veterinary; Mite Infestations/genetics; Polymorphism, Single Nucleotide; Genomics/methods; Genetic Predisposition to Disease; Genome-Wide Association Study/methods; Genome-Wide Association Study/veterinary; Psoroptidae/genetics; Cattle Diseases/genetics; Cattle; Cattle Diseases; Genome-Wide Association Study; Genomics; Mite Infestations; Psoroptidae; Ecology, Evolution, Behavior and Systematics; Animal Science and Zoology; Genetics
Abstract :
[en] [en] BACKGROUND: Psoroptic mange, caused by Psoroptes ovis mites, is affecting Belgian Blue cattle's welfare and production potential. The Belgian Blue cattle-known for its high degree of muscling, low feed conversion ratio and high beef quality-is highly susceptible for this disease.
RESULTS: In this study, we phenotyped 1975 Belgian Blue cattle from more than 100 different groups on commercial beef farms for their psoroptic mange susceptibility. Substantial individual differences were observed within these management groups, with lesion extent differences up to ± 15%. Animal models showed that estimated heritabilities were low for lesion extent and severe lesion extent (0.07 and 0.09, respectively) and 0.12 for the number of mites. A genome wide association study for mange susceptibility revealed signals on BTA6, BTA11, BTA15 and BTA24. In these regions, candidate genes GBA3, RAG2, and TRAF6 were identified.
CONCLUSIONS: Despite the challenges in phenotyping for psoroptic mange due to the timing of screening, the continuous evolution of lesions and different management conditions, we successfully conducted a study on the genetic susceptibility to psoroptic mange in Belgian Blue cattle. Our results clearly indicate that psoroptic mange is under polygenic control and the underlying candidate genes should be studied more thoroughly. This is the first study providing candidate genes for this complex disease. These results are already valuable for Belgian Blue breeding, however, further research is needed to unravel the architecture of this disease and to identify causal mutations.
Disciplines :
Veterinary medicine & animal health Genetics & genetic processes Agriculture & agronomy
Author, co-author :
Meyermans, Roel ; Center for Animal Breeding and Genetics, Department of Biosystems, KU Leuven, 3001, Leuven, Belgium. roel.meyermans@kuleuven.be
Janssens, Steven; Center for Animal Breeding and Genetics, Department of Biosystems, KU Leuven, 3001, Leuven, Belgium
Coussé, Annelies; Center for Animal Breeding and Genetics, Department of Biosystems, KU Leuven, 3001, Leuven, Belgium
Tinel, Susanne; Center for Animal Breeding and Genetics, Department of Biosystems, KU Leuven, 3001, Leuven, Belgium
Gorssen, Wim; Center for Animal Breeding and Genetics, Department of Biosystems, KU Leuven, 3001, Leuven, Belgium
FOD Volksgezondheid - Federale Overheidsdienst Volksgezondheid - Veiligheid van de Voetselketen en Leefmilieu FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen F.R.S.-FNRS - Fonds de la Recherche Scientifique KU Leuven - Catholic University of Leuven
Funding text :
The research that yielded these results was partly funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment through contracts RT 11/5 PSOROVIS and RT 17/1 BOMANGE. This research is also funded by a SB PhD fellowship (1S37119N) [RM], a FR PhD fellowship (1104320N) [WG] of the Research Foundation Flanders (FWO) and an Internal Funds grant of KU Leuven (PDMT2/23/035) [RM]. [TD] and [CC] are senior research associate and research director from the Fonds de la Recherche Scientifique\u2014FNRS (F.R.S.-FNRS).
L. Pouplard B. Losson M. Detry W. Hollanders Les gales bovines Ann Med Vet 1990 134 531 539
B. Losson J.F. Lonneux M. Lekimme The pathology of Psoroptesovis infestation in cattle with a special emphasis on breed difference Vet Parasitol 1999 83 219 229 1:STN:280:DyaK1MzkvFyltQ%3D%3D 10.1016/S0304-4017(99)00059-X 10423004
W.F. Fisher F.C. Wright Effects of the sheep scab mite on cumulative weight gains in cattle J Econ Entomol 1981 74 234 237 1:STN:280:DyaL38%2FpvVyrsw%3D%3D 10.1093/jee/74.2.234 7320316
J.F. Lonneux T. Nguyen J. Detry F. Farnir B. Losson The relationship between parasite counts, lesions, antibody titres and daily weight gains in Psoroptesovis infested cattle Vet Parasitol 1998 76 137 148 1:STN:280:DyaK1czhs1Gksg%3D%3D 10.1016/S0304-4017(97)00220-3 9653998
S. Rehbein M. Visser R. Winter B. Trommer H.F. Matthes A.E. Maciel et al. Productivity effects of bovine mange and control with ivermectin Vet Parasitol 2003 114 267 284 1:CAS:528:DC%2BD3sXksVGrs7s%3D 10.1016/S0304-4017(03)00140-7 12809753
C. Sarre K. De Bleecker P. Deprez B. Levecke J. Charlier J. Vercruysse et al. Risk factors for psoroptes ovis mange on Belgian Blue farms in Northern Belgium Vet Parasitol 2012 190 216 221 1:STN:280:DC%2BC38jjsVGhug%3D%3D 10.1016/j.vetpar.2012.05.026 22717121
C. Sarre A. González-Hernández S. Van Coppernolle R. Grit K. Grauwet F. Van Meulder et al. Comparative immune responses against Psoroptesovis in two cattle breeds with different susceptibility to mange Vet Res 2015 46 131 10.1186/s13567-015-0277-x 26582546 4652412
Z. Chen E. Claerebout K. Chiers M. Pas B. Pardon W. van Mol et al. Dermal immune responses against Psoroptesovis in two cattle breeds and effects of antiinflammatory dexamethasone treatment on the development of psoroptic mange Vet Res 2021 52 1 10.1186/s13567-020-00874-x 33397469 7784294
W. Van Mol N. De Wilde S. Casaert Z. Chen M. Vanhecke L. Duchateau et al. Resistance against macrocyclic lactones in Psoroptesovis in cattle Parasit Vectors 2020 13 127 10.1186/s13071-020-04008-2 32169109 7071684
N.O. Mapholi A. Maiwashe O. Matika V. Riggio S.C. Bishop M.D. MacNeil et al. Genome-wide association study of tick resistance in South African Nguni cattle Ticks Tick Borne Dis 2016 7 487 497 1:STN:280:DC%2BC28jislChsg%3D%3D 10.1016/j.ttbdis.2016.02.005 26897394
H.M. Burrow Variances and covariances between productive and adaptive traits and temperament in a composite breed of tropical beef cattle Livest Prod Sci 2001 70 213 233 10.1016/S0301-6226(01)00178-6
K. May K. Brügemann T. Yin C. Scheper C. Strube S. König Genetic line comparisons and genetic parameters for endoparasite infections and test-day milk production traits J Dairy Sci 2017 100 7330 7344 1:CAS:528:DC%2BC2sXhtFCgtLbF 10.3168/jds.2017-12901 28690064
A. Twomey R. Sayers R. Carroll N. Byrne E.O. Brien M. Doherty et al. Genetic parameters for both a liver damage phenotype caused by Fasciolahepatica and antibody response to Fasciolahepatica phenotype in dairy and beef cattle J Anim Sci 2016 94 4109 4119 1:CAS:528:DC%2BC2sXht1CiurrL 10.2527/jas.2016-0621 27898879
K. May C. Scheper K. Brügemann T. Yin C. Strube P. Korkuc et al. Genome-wide associations and functional gene analyses for endoparasite resistance in an endangered population of native German black pied cattle BMC Genomics 2019 20 277 10.1186/s12864-019-5659-4 30961534 6454736
R. Meyermans S. Janssens A. Coussé W. Gorssen X. Hubin P. Mayeres et al. Myostatin mutation causing double muscling could affect increased psoroptic mange sensitivity in dual purpose Belgian Blue cattle Animal 2022 16 1:CAS:528:DC%2BB38XhtVOqs7jO 10.1016/j.animal.2022.100460 35180681
F.S. Guillot Susceptibility of hereford cattle to sheep scab mites after recovery from psoroptic scabies J Econ Entomol 1981 74 653 657 1:STN:280:DyaL387itVOrsQ%3D%3D 10.1093/jee/74.6.653 7328214
C.C. Chang C.C. Chow L.C. Tellier S. Vattikuti S.M. Purcell J.J. Lee Second-generation PLINK: rising to the challenge of larger and richer datasets GigaScience 2015 4 7 10.1186/s13742-015-0047-8 25722852 4342193
C.A. Anderson F.H. Pettersson G.M. Clarke L.R. Cardon A.P. Morris K.T. Zondervan Data quality control in genetic case-control association studies Nat Protoc 2010 5 1564 1573 1:CAS:528:DC%2BC3cXht1aksLvN 10.1038/nprot.2010.116 21085122 3025522
I. Misztal S. Tsuruta D. Lourenco I. Aguilar A. Legarra Z. Vitezica Manual for BLUPF90 family of programs 2014 University of Georgia
D. Houle K. Meyer Estimating sampling error of evolutionary statistics based on genetic covariance matrices using maximum likelihood J Evol Biol 2015 28 1542 1549 1:STN:280:DC%2BC2MbjvVGguw%3D%3D 10.1111/jeb.12674 26079756
Z. Zhang F. Guillaume A. Sartelet C. Charlier M. Georges Farnir et al. Ancestral haplotype-based association mapping with generalized linear mixed models accounting for stratification Bioinformatics 2012 28 2467 2473 1:CAS:528:DC%2BC38XhsVehtbbN 10.1093/bioinformatics/bts348 22711794
S.R. Browning B.L. Browning Rapid and accurate haplotype phasing and missing-data inference for whole-genome association studies by use of localized haplotype clustering Am J Hum Genet 2007 81 1084 1097 1:CAS:528:DC%2BD2sXht1KmsL3M 10.1086/521987 17924348 2265661
T. Druet M. Georges A hidden markov model combining linkage and linkage disequilibrium information for haplotype reconstruction and QTL fine mapping Genetics 2010 184 789 798 1:CAS:528:DC%2BC3cXhsFOnurvN 10.1534/genetics.109.108431 20008575 2845346
S. Turner qqman: an R package for visualizing GWAS results using Q-Q and manhattan plots J Open Source Softw 2018 3 731 10.21105/joss.00731
S. Durinck Y. Moreau A. Kasprzyk S. Davis B. De Moor A. Brazma et al. Biomart and bioconductor: a powerful link between biological databases and microarray data analysis Bioinformatics 2005 21 3439 3440 1:CAS:528:DC%2BD2MXovVKru74%3D 10.1093/bioinformatics/bti525 16082012
S. Durinck P.T. Spellman E. Birney W. Huber Mapping identifiers for the integration of genomic datasets with the r/bioconductor package biomart Nat Protoc 2009 4 1184 1191 1:CAS:528:DC%2BD1MXpt12ksrk%3D 10.1038/nprot.2009.97 19617889 3159387
H. Schielzeth N.J. Dingemanse S. Nakagawa D.F. Westneat H. Alleue C. Teplitsky et al. Robustness of linear mixed-effects models to violations of distributional assumptions Methods Ecol Evol 2020 11 1141 1152 10.1111/2041-210X.13434
F. Coopman A. Van Zeveren G. Verhoeven S. De Smet Parameters for the estimation of live weight and for the visual appraisal of the muscular conformation in the (double-muscled) Belgian bLue beef breed Arch Anim Breed 2007 50 348 355 10.5194/aab-50-348-2007
E. Beutler L. Beutler C. West Mutations in the gene encoding cytosolic β-glucosidase in gaucher disease J Lab Clin Med 2004 144 65 68 1:CAS:528:DC%2BD2cXmvFWls7s%3D 10.1016/j.lab.2004.03.013 15322500
D.E. Boer J. van Smeden J.A. Bouwstra J.M. Aerts Glucocerebrosidase: functions in and beyond the lysosome J Clin Med 2020 9 736 1:CAS:528:DC%2BB3cXnvFShu74%3D 10.3390/jcm9030736 32182893 7141376
W. Holleran E. Ginns G. Menon J. Grundmann M. Fartasch P. Elias et al. Epidermal consequences of β-glucocerebrosidase deficiency: permeability barrier alterations and basis for skin lesions in type 2 gaucher disease J Clin Invest 1994 93 1756 1764 1:STN:280:DyaK2c3hs1Ghtw%3D%3D 10.1172/JCI117160 8163674 294236
S.L. Greene S.A. Muller Netherton’s syndrome: report of a case and review of the literature J Am Acad Dermatol 1985 13 329 337 1:STN:280:DyaL2M3ptVSrsg%3D%3D 10.1016/S0190-9622(85)70170-3 4031157
G. Imokawa A. Abe K. Jin Y. Higaki M. Kawashima A. Hidano Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin? J Invest Dermatol 1991 96 523 526 1:STN:280:DyaK3M7nvVejtw%3D%3D 10.1111/1523-1747.ep12470233 2007790
J.F. McBlane D.C. van Gent D.A. Ramsden C. Romeo C.A. Cuomo M. Gellert et al. Cleavage at a v(d)j recombination signal requires only rag1 and rag2 proteins and occurs in two steps Cell 1995 83 387 395 1:CAS:528:DyaK2MXpt1Oltr8%3D 10.1016/0092-8674(95)90116-7 8521468
S. Santagata A. Villa C. Sobacchi P. Cortes P. Vezzoni The genetic and biochemical basis of omenn syndrome Immunol Rev 2000 178 64 74 1:CAS:528:DC%2BD3MXoslCntw%3D%3D 10.1034/j.1600-065X.2000.17818.x 11213808
K. Aleman J.G. Noordzij R. de Groot J.J. van Dongen N.G. Hartwig Reviewing omenn syndrome Eur J Pediatr 2001 160 718 725 1:CAS:528:DC%2BD3MXosFGgurk%3D 10.1007/s004310100816 11795679
Y. Shinkai K.P. Lam E.M. Oltz V. Stewart M. Mendelsohn J. Charron et al. Rag-2- deficient mice lack mature lymphocytes owing to inability to initiate v(d)j rearrangement Cell 1992 68 855 867 1:CAS:528:DyaK38XitFaks7g%3D 10.1016/0092-8674(92)90029-C 1547487
O.M. Delmonte C. Schuetz L.D. Notarangelo RAG deficiency: two genes, many diseases J Clin Immunol 2018 38 646 655 1:CAS:528:DC%2BC1cXhsVeju7bO 10.1007/s10875-018-0537-4 30046960 6643099
M.C. Walsh J. Lee Y. Choi Tumor necrosis factor receptor associated factor 6 (TRAF6) regulation of development, function, and homeostasis of the immune system Immunol Rev 2015 266 72 92 1:CAS:528:DC%2BC2MXhtVOntrrN 10.1111/imr.12302 26085208 4799835
