[en] The cultivation of pineapple (Ananas comosus) is threatened worldwide by mealybug wilt disease of pineapple (MWP), whose etiology is not yet fully elucidated. In this study, we characterized pineapple mealybug wilt-associated ampeloviruses (PMWaVs, family Closteroviridae) from a diseased pineapple plant collected from Reunion Island, using a high-throughput sequencing approach combining Illumina short reads and Nanopore long reads. Reads co-assembly resulted in complete or near-complete genomes for six distinct ampeloviruses, including the first complete genome of pineapple mealybug wilt-associated virus 5 (PMWaV5) and that of a new species tentatively named pineapple mealybug wilt-associated virus 7 (PMWaV7). Short reads data provided high genome coverage and sequencing depths for all six viral genomes, contrary to long reads data. The 5′ and 3′ ends of the genome for most of the six ampeloviruses could be recovered from long reads, providing an alternative to RACE-PCRs. Phylogenetic analyses did not unveil any geographic structuring of the diversity of PMWaV1, PMWaV2 and PMWaV3 isolates, supporting the current hypothesis that PMWaVs were mainly spread by human activity and vegetative propagation.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Massé, Delphine; ANSES—LSV RAPT, F-97410 St. Pierre, La Réunion, France ; UMR PVBMT, Université de La Réunion, F-97410 St. Pierre, La Réunion, France
Candresse, Thierry ; INRAe, UMR 1332 Biologie du Fruit et Pathologie, Université Bordeaux, CS20032, F-33882 Villenave d’Ornon, France
Filloux, Denis; CIRAD, UMR PHIM, F-34090 Montpellier, France ; PHIM Plant Health Institute, Université Montpellier, CIRAD, INRAE, Institut Agro, IRD, F-34090 Montpellier, France
Massart, Sébastien ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Cassam, Nathalie; ANSES—LSV RAPT, F-97410 St. Pierre, La Réunion, France
Hostachy, Bruno; ANSES—LSV RAPT, F-97410 St. Pierre, La Réunion, France
Marais, Armelle ; INRAe, UMR 1332 Biologie du Fruit et Pathologie, Université Bordeaux, CS20032, F-33882 Villenave d’Ornon, France
Fernandez, Emmanuel; CIRAD, UMR PHIM, F-34090 Montpellier, France ; PHIM Plant Health Institute, Université Montpellier, CIRAD, INRAE, Institut Agro, IRD, F-34090 Montpellier, France
Roumagnac, Philippe ; CIRAD, UMR PHIM, F-34090 Montpellier, France ; PHIM Plant Health Institute, Université Montpellier, CIRAD, INRAE, Institut Agro, IRD, F-34090 Montpellier, France
Verdin, Eric; INRAe, UR407 Unité de Pathologie Végétale, CS 60094, F-84140 Montfavet, France
Teycheney, Pierre-Yves ; CIRAD, UMR PVBMT, F-97410 St. Pierre, La Réunion, France
Lett, Jean-Michel; CIRAD, UMR PVBMT, F-97410 St. Pierre, La Réunion, France
Lefeuvre, Pierre ; CIRAD, UMR PVBMT, F-97410 St. Pierre, La Réunion, France
EU - European Union Conseil Régional de La Réunion CIRAD - Centre de Coopération Internationale en Recherche Agronomique pour le Développement ANSES - Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail
FAOSTAT Food and Agriculture Organization of the United Nations FAOSTAT Statistics Database FAO Rome, Italy 2023
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