Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads.

Bouzar, Amel; Boxus, Mathieu; Florins, ARNAUD-FRANCOIS; Francois, Carole; Reichert, Michal; Willems, Luc

doi:10.1186/1742-4690-6-102

Article (Scientific journals)

Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads.

Bouzar, Amel; Boxus, Mathieu; Florins, ARNAUD-FRANCOIS et al.

2009 • In Retrovirology, 6, p. 102

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/31997

DOI
10.1186/1742-4690-6-102

PubMed
19903329

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Abstract :

[en] BACKGROUND: Bovine Leukemia virus (BLV) is a deltaretrovirus that induces lymphoproliferation and leukemia in ruminants. In ex vivo cultures of B lymphocytes isolated from BLV-infected sheep show that spontaneous apoptosis is reduced. Here, we investigated the involvement of reactive oxygen species (ROS) in this process. RESULTS: We demonstrate that (i) the levels of ROS and a major product of oxidative stress (8-OHdG) are reduced, while the thioredoxin antioxidant protein is highly expressed in BLV-infected B lymphocytes, (ii) induction of ROS by valproate (VPA) is pro-apoptotic, (iii) inversely, the scavenging of ROS with N-acetylcysteine inhibits apoptosis, and finally (iv) the levels of ROS inversely correlate with the proviral loads. CONCLUSION: Together, these observations underline the importance of ROS in the mechanisms of inhibition of apoptosis linked to BLV infection.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Bouzar, Amel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Boxus, Mathieu ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Florins, ARNAUD-FRANCOIS ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Francois, Carole ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Reichert, Michal

Willems, Luc ; Université de Liège - ULiège > GIGA-Research - Gembloux Agro-Bio Tech

Language :

English

Title :

Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads.

Publication date :

2009

Journal title :

Retrovirology

eISSN :

1742-4690

Publisher :

BioMed Central, London, United Kingdom

Volume :

Pages :

102

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 December 2009

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