Circulating hsa-miRNAs; Next generation sequencing; Sarcopenia; TaqMan qPCR; MicroRNAs; Male; Humans; Aged; Sarcopenia/genetics; MicroRNAs/genetics; MicroRNAs/metabolism; Aging; Geriatrics and Gerontology
Abstract :
[en] [en] OBJECTIVE: To identify a microRNA signature associated to sarcopenia in community-dwelling older adults form the SarcoPhAge cohort.
METHODS: In a screening phase by next generation sequencing (NGS), we compared the hsa-miRome expression of 18 subjects with sarcopenia (79.6 ± 6.8 years, 9 men) and 19 healthy subjects without sarcopenia (77.1 ± 6 years, 9 men) at baseline. Thereafter, we have selected eight candidate hsa-miRNAs according to the NGS results and after a critical assessment of previous literature. In a validation phase and by real-time qPCR, we then analyzed the expression levels of these 8 hsa-miRNAs at baseline selecting 92 healthy subjects (74.2 ± 10 years) and 92 subjects with sarcopenia (75.3 ± 6.8 years). For both steps, the groups were matched for age and sex.
RESULTS: In the validation phase, serum has-miRNA-133a-3p and has-miRNA-200a-3p were significantly decreased in the group with sarcopenia vs controls [RQ: relative quantification; median (interquartile range)]: -0.16 (-1.26/+0.90) vs +0.34 (-0.73/+1.33) (p < 0.01) and -0.26 (-1.07/+0.68) vs +0.27 (-0.55/+1.10) (p < 0.01) respectively. Has-miRNA-744-5p was decreased and has-miRNA-151a-3p was increased in the group with sarcopenia vs controls, but this barely reached significance: +0.16 (-1.34/+0.79) vs +0.44 (-0.31/+1.00) (p = 0.050) and +0.35 (-0.22/+0.90) vs +0.03 (-0.68/+0.75) (p = 0.054).
CONCLUSION: In subjects with sarcopenia, serum hsa-miRNA-133a-3p and hsa-miRNA-200a-3p expression were downregulated, consistent with their potential targets inhibiting muscle cells proliferation and differentiation.
Disciplines :
Public health, health care sciences & services Laboratory medicine & medical technology
Author, co-author :
Millet, Marjorie; INSERM 1033, Lyon, France ; PMO, Lyon, France
Auroux, Maxime; INSERM 1033, Lyon, France ; Hôpital E. Herriot, Hospices Civils de Lyon, Lyon, France
Beaudart, Charlotte ; Université de Liège - ULiège > Département des sciences de la santé publique > Epidémiologie générale ; Clinical Pharmacology and Toxicology Research Unit (URPC), NARILIS, Department of Biomedical Sciences, Faculty of Medicine, University of Namur, Namur, Belgium
Demonceau, Céline ; Université de Liège - ULiège > Unité de recherche Santé publique, épidémiologie et économie de la santé (URSAPES)
Ladang, Aurélie ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Reginster, Jean-Yves ; Université de Liège - ULiège > Département des sciences de la santé publique
Bruyère, Olivier ; Université de Liège - ULiège > Département des sciences de la santé publique > Santé publique, Epidémiologie et Economie de la santé
Chapurlat, Roland; INSERM 1033, Lyon, France ; PMO, Lyon, France ; Hôpital E. Herriot, Hospices Civils de Lyon, Lyon, France ; University of Lyon, Lyon, France
Rousseau, Jean-Charles ; INSERM 1033, Lyon, France. jean-charles.rousseau@inserm.fr ; PMO, Lyon, France. jean-charles.rousseau@inserm.fr
Language :
English
Title :
Association of circulating hsa-miRNAs with sarcopenia: the SarcoPhAge study.
Publication date :
14 March 2024
Journal title :
Aging Clinical and Experimental Research
ISSN :
1594-0667
eISSN :
1720-8319
Publisher :
Springer Science and Business Media Deutschland GmbH, Germany
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