RGB sensors; chemical analysis; colorimetric characterisation; optical sensor; Liquid analyte; Measurement system; Optical path; RGB sensor; Analytical Chemistry; Atomic and Molecular Physics, and Optics; Instrumentation; Electrical and Electronic Engineering
Abstract :
[en] Colorimetric characterisation systems based on LEDs and RBG sensors are straightforward to implement, are highly integrable allowing for portable measurement systems and can be constructed using widespread and affordable components. They have already proved to be a satisfactory solution in several applications related to chemical analysis. In this paper, we present an RGB sensor-based prototype for colorimetric characterisation, which can accommodate cuvettes with optical paths of 10 mm and 40 mm. We assessed the impact of experimental condition parameters such as the variability of the analyte volume in the cuvette, as well as the presence of floating particles or deposits at the bottom of the cuvette. While these would not impact the result given by a spectrophotometer that generally has a directional light source, they must be considered in LED/RGB sensor analysers in which the light path is not tightly controlled. We demonstrated that there is a minimal sensor height above the bottom of the cuvette and a minimal analyte level (both depending on the prototype optical path length) above which the analyte volume and the presence of floating particles and deposits have no impact on the prototype output signal. Finally, based on these results, we proposed a test method for a quick dye-displacement assay, in which the reagent is a dye-loaded molecularly imprinted polymer that is poured directly into a cuvette.
Disciplines :
Electrical & electronics engineering
Author, co-author :
Dupont, François ; Université de Liège - ULiège > Département d'électricité, électronique et informatique (Institut Montefiore) > Systèmes microélectroniques intégrés
Stoukatch, Serguei ; Université de Liège - ULiège > Département d'électricité, électronique et informatique (Institut Montefiore) > Systèmes microélectroniques intégrés
Laurent, Philippe ; Université de Liège - ULiège > Département d'électricité, électronique et informatique (Institut Montefiore) > Systèmes microélectroniques intégrés
Eersels, Kasper ; Sensor Engineering Department, Faculty of Science and Engineering, Maastricht University, 6200 MD Maastricht, The Netherlands
van Grinsven, Bart ; Sensor Engineering Department, Faculty of Science and Engineering, Maastricht University, 6200 MD Maastricht, The Netherlands
Redouté, Jean-Michel ; Université de Liège - ULiège > Département d'électricité, électronique et informatique (Institut Montefiore) > Systèmes microélectroniques intégrés
Language :
English
Title :
Design Aspects for Portable LED-Based Colorimetric Characterisation Systems Targeting Liquid Analytes.
Publication date :
19 March 2024
Journal title :
Sensors
ISSN :
1424-8220
eISSN :
1424-3210
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Interreg EMR - Interreg Euregio Meuse-Rhin [NL] Walloon region [BE] EZK - Ministerie van Economische Zaken en Klimaat [NL]
Funding text :
The work was carried out in the frame of the Interreg EMR Food_Screening project (EMR159). It was supported by Interreg Euregio Meuse-Rhin, the Walloon Region of Belgium and the Ministerie van Economische Zaken en Klimaat. The whole project was also supported by Provincie Limburg and Die Landesregierung Nordrhein-Westfalen. This project was carried out in collaboration with a consortium of research institutions and SMEs in Belgium, the Netherlands and Germany.
Biswas P.C. Rani S. Hossain M.A. Islam M.R. Canning J. Multichannel Smartphone Spectrometer Using Combined Diffraction Orders IEEE Sens. Lett. 2020 4 4500804 10.1109/LSENS.2020.3015590
Gonzalez-Moralez D.F. Lopez-Santos O. Garcia-Beltran O. Low-Cost Spectrophotometer for In-Situ Detection of Mercury in Water Proceedings of the 2018 IEEE SENSORS New Delhi, India 28–31 October 2018 1 4
de Carvalho Oliveira G. Machado C.C.S. Inácio D.K. da Silveira Petruci J.F. Silva S.G. RGB color sensor for colorimetric determinations: Evaluation and quantitative analysis of colored liquid samples Talanta 2022 241 123244 10.1016/j.talanta.2022.123244 35121545
Danh L.V.Q. Thien D.C. Binh Q.D. Dieu N.T. Hoan T.N.K. A Low-Cost Colorimetric Sensor for On-Site Automatic Monitoring of Nitrite in Aquaculture Proceedings of the 2021 IEEE 7th International Conference on Smart Instrumentation, Measurement and Applications (ICSIMA) Bandung, Indonesia 23–25 August 2021 117 122
Cadeado A.N. Machado C.C. Costa M.Q. Silva S.G. A palm-sized wireless device for colorimetric nitrite determination in water Microchem. J. 2022 183 108138 10.1016/j.microc.2022.108138
Debarshi S. Khan M.M. Portable and low-cost LED based Spectrophotometer for the Detection of Nitrite in simulated-Urine Proceedings of the 2019 International Conference on Electrical, Electronics and Computer Engineering (UPCON) Aligarh, India 8–10 November 2019 1 4
Kim J.-S. Oh H.-B. Kim A.-H. Kim J.-S. Lee E.-S. Goh B.-J. Choi J.-H. Shin Y.-J. Baek J.-Y. Lee K.-S. et al. An array-Type RGB Sensor for Precision Measurement of pH J. Opt. Soc. Korea 2015 19 700 704 10.3807/JOSK.2015.19.6.700
Stoukatch S. Debliquy M. Dupont F. Redouté J.M. Low-Cost Optical pH Sensor with a Polyaniline (PANI)-Sensitive Layer Based on Commercial off-the-Shelf (COTS) Components Micromachines 2023 14 2197 10.3390/mi14122197 38138366
Sampedro O. Salgueiro J.R. Turbidimeter and RGB sensor for remote measurements in an aquatic medium Measurement 2015 68 128 134 10.1016/j.measurement.2015.02.049
Da Nobrega Gaiao E. Bezerra dos Santos S.R. Bezerra dos Santos V. Lima do Nascimento E.C. Souza Lima R. Ugulino de Araujo M.C. An inexpensive, portable and microcontrolled near infrared LED-photometer for screening analysis of gasoline Talanta 2008 75 792 796 10.1016/j.talanta.2007.12.014 18585148
“Color Analysis for Pharmaceutical Products Using UV-Visible Absorption Technique”, App. Note, Thermo Scientific Available online: https://assets.thermofisher.com/TFS-Assets/MSD/Application-Notes/color-analysis-pharmaceutical-an56364-en.pdf (accessed on 2 October 2023)
da Silva Magalhães T. Reis B.F. A novel multicommuted flow analysis strategy for the spectrophotometric determination of cadmium in water at µg L-1 levels without using a preconcentration step Anal. Methods 2018 10 900 909 10.1039/C7AY02644G
VLMW33S2V1-5K8L-08 Datasheet Available online: https://www.vishay.com/docs/81273/vlmw33.pdf (accessed on 8 March 2024)
VEML3328 Application Note Available online: https://www.vishay.com/docs/80010/designingveml3328.pdf (accessed on 8 March 2024)
Azzam R.M.A. High-index dielectric substrates with nearly constant reflectance for incident unpolarized or circularly polarized light over a wide range of incidence angles J. Mod. Opt. 2015 62 811 815 10.1080/09500340.2015.1007103
Pope R.M. Fry E.S. Absorption spectrum (380–700 nm) of pure water, I.I. Integrating cavity measurements Appl. Opt. 1997 36 8710 8723 10.1364/AO.36.008710 18264420
Martinez Gache S.A. Recoulat Angelini A.A. Sabeckis M.L. Gonzales Flecha F.L. Improving the stability of the malachite green method for determination of phosphate using Pluronic P68 Anal. Biochem. 2020 597 113681 10.1016/j.ab.2020.113681 32165102
Denman S. Jameel S. Hay J. Sugden J.K. Photostability of crystal violet (CI 42555) Dye. Pigment. 1996 30 67 72 10.1016/0143-7208(95)00067-4
Erstad A.J. Erstad B.L. Nix D.E. Accuracy and reproducibility of small-volume injections from various-sized syringes Am. J. Health-Syst. Pharm. 2006 63 748 750 10.2146/ajhp050438 16595816
Goosens J. Vandenryt T. Lowdon J.W. van Grinsven B. Eersels K. Deferme W. Thoelen R. Design of a Lab-on-Chip Cartridge for the Optical Detection of Small Molecules Based on Dye-Displacement MIPs IEEE Trans. Instrum. Meas. 2023 72 9511209 10.1109/TIM.2023.3301040