The Protective Effects of Lavender officinalis Extract Against Impairment of Antioxidant–Detoxification System Induced by Glucose Deprivation Through Nrf2 Expression
[en] Despite tremendous progress in the prevention and treatment of cerebral ischemia, stroke remains one of the major causes of mortality and paralysis among the elderly. The design and study of neuroprotective drugs in vitro, such as in the Serum-Glucose Deprivation (SGD) condition, are proved to be efficient to understand how these drugs defend neuronal cells from ischemia-induced damage. Neurological defects and stroke volume are reduced by lavender plant’s potent and abundant compounds reduces. In this study, we intend to study the mechanism of a protective effect of the lavender extract on insult caused by glucose deprivation in the PC12 cell line, focusing on the Nrf2 pathway. In order to investigate the protective effects of the extract, the cells were subjected to serum/glucose deprivation for a period of 12 h at non-toxic concentrations and viability was investigated. Expression levels of Nrf2, HO-1, and NQO1 genes were evaluated. Also, the activity of CAT, SOD, and GPx enzymes was measured. The results illustrate an increase in cell viability, expression of HO-1 and NQO1 genes, and enzymatic activity of CAT, SOD, GPx. In conclusion, pretreatment of PC12 cells with hydroalcoholic extract of lavender reinforces antioxidant system and detoxification enzymes under oxidative stress.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kouhihabibidehkordi, Golnoush; Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Ghanavatinejad, Fatemeh ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques ; Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Taheri, Koosha; Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Soleimannejad, Mostafa; Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran ; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
Lorigooini, Zahra; Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Amini-Khoei, Hossein; Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Mobini, GholamReza ; Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran ; Cancer Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Language :
English
Title :
The Protective Effects of Lavender officinalis Extract Against Impairment of Antioxidant–Detoxification System Induced by Glucose Deprivation Through Nrf2 Expression
Publication date :
February 2024
Journal title :
Proceedings of the National Academy of Sciences, India. Section B, Biological Sciences
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