[en] A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging-drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 angstrom, and a data set was collected to 2.76 angstrom.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Maes, D.
Crabeel, M.
Van de Weerdt, Cécile ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Peeters, E.
Charlier, Daniël
Decanniere, K.
Vanhee, C.
Wyns, L.
Zegers, I.
Language :
English
Title :
Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study
Publication date :
December 2006
Journal title :
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
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