[en] A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging-drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 angstrom, and a data set was collected to 2.76 angstrom.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Maes, D.
Crabeel, M.
Van de Weerdt, Cécile ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Peeters, E.
Charlier, Daniël
Decanniere, K.
Vanhee, C.
Wyns, L.
Zegers, I.
Language :
English
Title :
Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study
Publication date :
December 2006
Journal title :
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Abadjieva, A., Hilven, P., Pauwels, K. & Crabeel, M. (2000). J. Biol. Chem. 275, 11361-11367.
Crabeel, M., Abadjieva, A., Hilven, P., Desimpelaere, J. & Soetens, O. (1997). Eur. J. Biochem. 250, 232-241.
Elkins, J. M., Kershaw, N. J. & Schofield, C. J. (2005). Biochem. J. 385, 565-573.
García-Ruiz, J. M. (2003). Methods Enzymol. 368, 130-154.
García-Ruiz, J. M., Gonzalez-Ramirez, L. A., Gavira, J. A. & Otálora, F. (2002). Acta Cryst. D58, 1638-1642.
García-Ruiz, J. M., Otálora, F., Novella, M. L., Gavira, J. A., Sauter, C. & Vidal, O. (2001). J. Cryst. Growth, 232, 149-155.
Giegé, R., Drenth, J., Ducruix, A., McPherson, A. & Saenger, W. (1995). Prog. Cryst. Growth Charact. Mater. 30, 237-281.
Kershaw, N. J., McNaughton, H. J., Hewitson, K. S., Hernandez, H., Griffin, J., Hughes, C., Greaves, P., Barton, B., Robinson, C. V. & Schofield, C. J. (2002). Eur. J. Biochem. 269, 2052-2059.
Liu, Y., Van Heeswijck, R., Hoj, P. & Hoogenraad, N. (1995). Eur. J. Biochem. 228, 291-296.
McPherson, A. (1993). J. Phys. D, 26, B104-B112.
Maes, D., Gonzalez-Ramirez, L. A., Lopez-Jaramillo, J., Yu, B., De Bondt, H., Zegers, I., Afonina, E., García-Ruiz, J. M. & Gulnik, S. (2004). Acta Cryst. D60, 463-471.
Marc, F., Weigel, P., Legrain, C., Glansdorff, N. & Sakanyan, V. (2001). J. Biol. Chem. 276, 25404-25410.
Ng, J. D., Gavira, J. A. & García-Ruiz, J. M. (2003). J. Struct. Biol. 142, 218-231.
Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
Rosenberger, F. (1986). J. Cryst. Growth, 76, 618-636.
Sakanyan, V., Charlier, D., Legrain, C., Kochikyan, A., Mett, I., Pierard, A. & Glansdorff, N. (1993). J. Gen. Microbiol. 139, 393-402.
Snell, E. H. & Helliwell, J. R. (2005). Rep. Prog. Phys. 68, 799-853.
Thomas, B. R., Chernov, A. A., Vekilov, P. G. & Carter, D. C. (2000). J. Cryst. Growth, 211, 149-156.
Vekilov, P. G. (1999). Adv. Space Res. 24, 1231-1240.
Vekilov, P. G. & Chernov, A. A. (2002). Solid State Phys. Adv. Res. Appl. 57, 1-147.
Vergara, A., Lorber, B., Zagari, A. & Giegé, R. (2003). Acta Cryst. D59, 2-15.
Vergara, A., Paduano, L., D'Errico, G. & Sartorio, R. (1999). Phys. Chem. Chem. Phys. 1, 4875-4879.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
