[en] Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Welsh, Joshua A ; Translational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
Goberdhan, Deborah C I ; Nuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, John Radcliffe Hospital, Oxford, UK
O'Driscoll, Lorraine; School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland ; Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland ; Trinity St. James's Cancer Institute, Trinity College Dublin, Dublin, Ireland
Buzas, Edit I ; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary ; HCEMM-SU Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary ; HUN-REN-SU Translational Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary
Blenkiron, Cherie; Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand
Bussolati, Benedetta ; Department of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy
Cai, Houjian ; University of Georgia, Athens, Georgia, USA
Di Vizio, Dolores; Department of Surgery, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, California, USA
Driedonks, Tom A P; Department CDL Research, University Medical Center Utrecht, Utrecht, The Netherlands
Erdbrügger, Uta; University of Virginia Health System, Charlottesville, Virginia, USA
Falcon-Perez, Juan M ; Exosomes Laboratory, Center for Cooperative Research in Biosciences, Basque Research and Technology Alliance, Derio, Spain ; Metabolomics Platform, Center for Cooperative Research in Biosciences, Basque Research and Technology Alliance, Derio, Spain ; IKERBASQUE, Basque Foundation for Science, Bilbao, Spain
Fu, Qing-Ling; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China ; Extracellular Vesicle Research and Clinical Translational Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Hill, Andrew F ; Institute for Health and Sport, Victoria University, Melbourne, Australia
Lenassi, Metka ; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Lim, Sai Kiang; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore ; Paracrine Therapeutics Pte. Ltd., Singapore, Singapore ; Department of Surgery, YLL School of Medicine, National University Singapore, Singapore, Singapore
Mahoney, Mỹ G; Thomas Jefferson University, Philadelphia, Pennsylvania, USA
Mohanty, Sujata ; Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India
Möller, Andreas; Chinese University of Hong Kong, Hong Kong, Hong Kong S.A.R ; QIMR Berghofer Medical Research Institute, Brisbane, Australia
Nieuwland, Rienk ; Laboratory of Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands ; Amsterdam Vesicle Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands
Ochiya, Takahiro ; Tokyo Medical University, Tokyo, Japan
Sahoo, Susmita ; Icahn School of Medicine at Mount Sinai, New York, New York, USA
Torrecilhas, Ana C ; Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo (UNIFESP) Campus Diadema, Diadema, Brazil
Zheng, Lei ; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
Zijlstra, Andries ; Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA ; Genentech, South San Francisco, California, USA
Abuelreich, Sarah; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA
Bagabas, Reem; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA
Bergese, Paolo; Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy ; Center for Colloid and Surface Science (CSGI), Florence, Italy ; National Center for Gene Therapy and Drugs based on RNA Technology, Padua, Italy
Bridges, Esther M; Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
Brucale, Marco ; Consiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati, Bologna, Italy ; Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Florence, Italy
Burger, Dylan; Kidney Research Centre, Ottawa Hopsital Research Institute, Ottawa, Canada ; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada ; School of Pharmaceutical Sciences, University of Ottawa, Ottawa, Canada
Carney, Randy P ; Department of Biomedical Engineering, University of California, Davis, California, USA
Cocucci, Emanuele; Division of Pharmaceutics and Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio, USA ; Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA
Crescitelli, Rossella ; Sahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden ; Wallenberg Centre for Molecular and Translational Medicine, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Hanser, Edveena; Department of Biomedicine, University Hospital Basel, Basel, Switzerland ; Department of Biomedicine, University of Basel, Basel, Switzerland
Harris, Adrian L; Department of Oncology, University of Oxford, Oxford, UK
Haughey, Norman J ; Departments of Neurology and Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Hendrix, An; Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium ; Cancer Research Institute Ghent, Ghent, Belgium
Ivanov, Alexander R; Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA
Jovanovic-Talisman, Tijana; Department of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA
Kruh-Garcia, Nicole A; Bio-pharmaceutical Manufacturing and Academic Resource Center (BioMARC), Infectious Disease Research Center, Colorado State University, Fort Collins, Colorado, USA
Ku'ulei-Lyn Faustino, Vroniqa; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA
Kyburz, Diego; Department of Biomedicine, University of Basel, Basel, Switzerland ; Department of Rheumatology, University Hospital Basel, Basel, Switzerland
Lässer, Cecilia; Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Lennon, Kathleen M; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA
Lötvall, Jan; Krefting Research Centre, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Maddox, Adam L; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA
Martens-Uzunova, Elena S ; Erasmus MC Cancer Institute, University Medical Center Rotterdam, Department of Urology, Rotterdam, The Netherlands
Mizenko, Rachel R; Department of Biomedical Engineering, University of California, Davis, California, USA
Newman, Lauren A ; College of Medicine and Public Health, Flinders University, Adelaide, Australia
Ridolfi, Andrea; Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Rohde, Eva ; Department of Transfusion Medicine, University Hospital, Salzburger Landeskliniken GmbH of Paracelsus Medical University, Salzburg, Austria ; GMP Unit, Paracelsus Medical University, Salzburg, Austria ; Transfer Centre for Extracellular Vesicle Theralytic Technologies, EV-TT, Salzburg, Austria
Rojalin, Tatu; Department of Biomedical Engineering, University of California, Davis, California, USA ; Expansion Therapeutics, Structural Biology and Biophysics, Jupiter, Florida, USA
Rowland, Andrew; College of Medicine and Public Health, Flinders University, Adelaide, Australia
Saftics, Andras; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA
Sandau, Ursula S ; Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA
Saugstad, Julie A ; Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA
Shekari, Faezeh ; Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran ; Celer Diagnostics, Toronto, Canada
Swift, Simon; Waipapa Taumata Rau University of Auckland, Auckland, New Zealand
Ter-Ovanesyan, Dmitry; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA
Tosar, Juan P ; Universidad de la República, Montevideo, Uruguay ; Institut Pasteur de Montevideo, Montevideo, Uruguay
Useckaite, Zivile; College of Medicine and Public Health, Flinders University, Adelaide, Australia
Valle, Francesco; Consiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati, Bologna, Italy ; Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Florence, Italy
Varga, Zoltan; Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, Hungary ; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
van der Pol, Edwin; Amsterdam Vesicle Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands ; Biomedical Engineering and Physics, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, The Netherlands ; Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, The Netherlands
van Herwijnen, Martijn J C; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
Wauben, Marca H M ; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
Wehman, Ann M ; University of Denver, Denver, USA
Williams, Sarah; International Society for Extracellular Vesicles
Zendrini, Andrea; Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy ; Center for Colloid and Surface Science (CSGI), Florence, Italy
Zimmerman, Alan J ; Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA
Njock, Makon-Sébastien ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie
Théry, Clotilde ; Institut Curie, INSERM U932, PSL University, Paris, France ; CurieCoreTech Extracellular Vesicles, Institut Curie, Paris, France
Witwer, Kenneth W ; Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA ; EV Core Facility "EXCEL", Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA ; The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Aalberts, M., van Dissel-Emiliani, F. M., van Adrichem, N. P., van Wijnen, M., Wauben, M. H., Stout, T. A., & Stoorvogel, W. (2012). Identification of distinct populations of prostasomes that differentially express prostate stem cell antigen, annexin A1, and GLIPR2 in humans. Biology of Reproduction, 86, 82.
Aasebø, E., Opsahl, J. A., Bjørlykke, Y., Myhr, K. M., Kroksveen, A. C., & Berven, F. S. (2014). Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome. PLoS ONE, 9, e90429.
Abbatiello, S. E., Mani, D. R., Schilling, B., Maclean, B., Zimmerman, L. J., Feng, X., Cusack, M. P., Sedransk, N., Hall, S. C., Addona, T., Allen, S., Dodder, N. G., Ghosh, M., Held, J. M., Hedrick, V., Inerowicz, H. D., Jackson, A., Keshishian, H., Kim, J. W., … Carr, S. A. (2013). Design, implementation and multisite evaluation of a system suitability protocol for the quantitative assessment of instrument performance in liquid chromatography-multiple reaction monitoring-MS (LC-MRM-MS). Molecular & Cellular Proteomics, 12, 2623–2639.
Aebersold, R., & Mann, M. (2003). Mass spectrometry-based proteomics. Nature, 422, 198–207.
Aguet, F., Antonescu, C. N., Mettlen, M., Schmid, S. L., & Danuser, G. (2013). Advances in analysis of low signal-to-noise images link dynamin and AP2 to the functions of an endocytic checkpoint. Developmental Cell, 26, 279–291.
Ainsztein, A. M., Brooks, P. J., Dugan, V. G., Ganguly, A., Guo, M., Howcroft, T. K., Kelley, C. A., Kuo, L. S., Labosky, P. A., Lenzi, R., McKie, G. A., Mohla, S., Procaccini, D., Reilly, M., Satterlee, J. S., Srinivas, P. R., Church, E. S., Sutherland, M., Tagle, D. A., … Venkatachalam, S. (2015). The NIH extracellular RNA communication consortium. Journal of Extracellular Vesicles, 4, 27493.
Alexander, M., Hu, R., Runtsch, M. C., Kagele, D. A., Mosbruger, T. L., Tolmachova, T., Seabra, M. C., Round, J. L., Ward, D. M., & O'Connell, R. M. (2015). Exosome-delivered microRNAs modulate the inflammatory response to endotoxin. Nature Communications, 6, 7321.
Ambrose, A. R., Dechantsreiter, S., Shah, R., Montero, M. A., Quinn, A. M., Hessel, E. M., Beinke, S., Tannahill, G. M., & Davis, D. M. (2020). Corrected super-resolution microscopy enables nanoscale imaging of autofluorescent lung macrophages. Biophysical Journal, 119, 2403–2417.
Aps, J. K., & Martens, L. C. (2005). Review: The physiology of saliva and transfer of drugs into saliva. Forensic Science International, 150, 119–131.
Arab, T., Mallick, E. R., Huang, Y., Dong, L., Liao, Z., Zhao, Z., Gololobova, O., Smith, B., Haughey, N. J., Pienta, K. J., Slusher, B. S., Tarwater, P. M., Tosar, J. P., Zivkovic, A. M., Vreeland, W. N., Paulaitis, M. E., & Witwer, K. W. (2021). Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms. Journal of Extracellular Vesicles, 10, e12079.
Arifin, D. R., Witwer, K. W., & Bulte, J. W. M. (2022). Non-Invasive imaging of extracellular vesicles: Quo vaditis in vivo? Journal of Extracellular Vesicles, 11, e12241.
Arigony, A. L., de Oliveira, I. M., Machado, M., Bordin, D. L., Bergter, L., Prá, D., & Henriques, J. A. (2013). The influence of micronutrients in cell culture: A reflection on viability and genomic stability. BioMed Research International, 2013, 597282.
Arraud, N., Linares, R., Tan, S., Gounou, C., Pasquet, J. M., Mornet, S., & Brisson, A. R. (2014). Extracellular vesicles from blood plasma: Determination of their morphology, size, phenotype and concentration. Journal of Thrombosis and Haemostasis, 12, 614–627.
Avalos-Padilla, Y., Georgiev, V. N., Lantero, E., Pujals, S., Verhoef, R., Borgheti-Cardoso L, N., Albertazzi, L., Dimova, R., & Fernàndez-Busquets, X. (2021). The ESCRT-III machinery participates in the production of extracellular vesicles and protein export during Plasmodium falciparum infection. Plos Pathogens, 17, e1009455.
Bachurski, D., Schuldner, M., Nguyen, P. H., Malz, A., Reiners, K. S., Grenzi, P. C., Babatz, F., Schauss, A. C., Hansen, H. P., Hallek, M., & Pogge von Strandmann, E. (2019). Extracellular vesicle measurements with nanoparticle tracking analysis – An accuracy and repeatability comparison between NanoSight NS300 and ZetaView. Journal of Extracellular Vesicles, 8, 1596016.
Bai, L., Du, Y., Peng, J., Liu, Y., Wang, Y., Yang, Y., & Wang, C. (2014). Peptide-based isolation of circulating tumor cells by magnetic nanoparticles. Journal of Materials Chemistry B, 2, 4080–4088.
Balaj, L., Atai, N. A., Chen, W., Mu, D., Tannous, B. A., Breakefield, X. O., Skog, J., & Maguire, C. A. (2015). Heparin affinity purification of extracellular vesicles. Scientific Reports, 5, 10266.
Balaj, L., Lessard, R., Dai, L., Cho, Y. J., Pomeroy, S. L., Breakefield, X. O., & Skog, J. (2011). Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences. Nature Communications, 2, 180.
Baldrich, P., Rutter, B. D., Karimi, H. Z., Podicheti, R., Meyers, B. C., & Innes, R. W. (2019). Plant extracellular vesicles contain diverse small RNA species and are enriched in 10- to 17-nucleotide “Tiny” RNAs. Plant Cell, 31, 315–324.
Ballard, O., & Morrow, A. L. (2013). Human milk composition: Nutrients and bioactive factors. Pediatric Clinics of North America, 60, 49–74.
Banigan, M. G., Kao, P. F., Kozubek, J. A., Winslow, A. R., Medina, J., Costa, J., Schmitt, A., Schneider, A., Cabral, H., Cagsal-Getkin, O., Vanderburg, C. R., & Delalle, I. (2013). Differential expression of exosomal microRNAs in prefrontal cortices of schizophrenia and bipolar disorder patients. PLoS ONE, 8, e48814.
Barreiro, K., Dwivedi, O. P., Valkonen, S., Groop, P. H., Tuomi, T., Holthofer, H., Rannikko, A., Yliperttula, M., Siljander, P., Laitinen, S., Serkkola, E., Af Hallstrom, T., Forsblom, C., Groop, L., & Puhka, M. (2021). Urinary extracellular vesicles: Assessment of pre-analytical variables and development of a quality control with focus on transcriptomic biomarker research. Journal of Extracellular Vesicles, 10, e12158.
Beale, D. J., Jones, O. A., Karpe, A. V., Dayalan, S., Oh, D. Y., Kouremenos, K. A., Ahmed, W., & Palombo, E. A. (2016). A review of analytical techniques and their application in disease diagnosis in breathomics and salivaomics research. International Journal of Molecular Sciences, 18, 24.
Beckett, K., Monier, S., Palmer, L., Alexandre, C., Green, H., Bonneil, E., Raposo, G., Thibault, P., Borgne, R. L., & Vincent, J. P. (2013). Drosophila S2 cells secrete wingless on exosome-like vesicles but the wingless gradient forms independently of exosomes. Traffic (Copenhagen, Denmark), 14, 82–96.
Beer, K. B., Rivas-Castillo, J., Kuhn, K., Fazeli, G., Karmann, B., Nance, J. F., Stigloher, C., & Wehman, A. M. (2018). Extracellular vesicle budding is inhibited by redundant regulators of TAT-5 flippase localization and phospholipid asymmetry. PNAS, 115, E1127–E1136.
Beer, K. B., & Wehman, A. M. (2017). Mechanisms and functions of extracellular vesicle release in vivo-What we can learn from flies and worms. Cell Adhesion & Migration, 11, 135–150.
Benedikter, B. J., Bouwman, F. G., Vajen, T., Heinzmann, A. C. A., Grauls, G., Mariman, E. C., Wouters, E. F. M., Savelkoul, P. H., Lopez-Iglesias, C., Koenen, R. R., Rohde, G. G. U., & Stassen, F. R. M. (2017). Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies. Scientific Reports, 7, 15297.
Benmoussa, A., Michel, S., Gilbert, C., & Provost, P. (2020). Isolating multiple extracellular vesicles subsets, including exosomes and membrane vesicles, from bovine milk using sodium citrate and differential ultracentrifugation. Bio-Protocol, 10, e3636.
Bereman, M. S. (2015). Tools for monitoring system suitability in LC MS/MS centric proteomic experiments. Proteomics, 15, 891–902.
Berne, B. J., & Pecora, R. (1976). Dynamic light scattering: With applications to chemistry, biology, and physics. Wiley.
Bettin, B., Gasecka, A., Li, B., Dhondt, B., Hendrix, A., Nieuwland, R., & van der Pol, E. (2022). Removal of platelets from blood plasma to improve the quality of extracellular vesicle research. Journal of Thrombosis and Haemostasis, 20, 2679–2685.
Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J., & Hess, H. F. (2006). Imaging intracellular fluorescent proteins at nanometer resolution. Science, 313, 1642–1645.
Bhattarai, K. R., Kim, H. R., & Chae, H. J. (2018). Compliance with saliva collection protocol in healthy volunteers: strategies for managing risk and errors. International journal of Medical Sciences, 15, 823–831.
Bitto, N. J., Chapman, R., Pidot, S., Costin, A., Lo, C., Choi, J., D'Cruze, T., Reynolds, E. C., Dashper, S. G., Turnbull, L., Whitchurch, C. B., Stinear, T. P., Stacey, K. J., & Ferrero, R. L. (2017). Bacterial membrane vesicles transport their DNA cargo into host cells. Scientific Reports, 7, 7072.
Bitto, N. J., Cheng, L., Johnston, E. L., Pathirana, R., Phan, T. K., Poon, I. K. H., O'Brien-Simpson, N. M., Hill, A. F., Stinear, T. P., & Kaparakis-Liaskos, M. (2021). Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy. Journal of Extracellular Vesicles, 10, e12080.
Bitto, N. J., & Kaparakis-Liaskos, M. (2022). Methods of bacterial membrane vesicle production, purification, quantification, and examination of their immunogenic functions. Methods in Molecular Biology, 2523, 43–61.
Bitto, N. J., Zavan, L., Johnston, E. L., Stinear, T. P., Hill, A. F., & Kaparakis-Liaskos, M. (2021). Considerations for the analysis of bacterial membrane vesicles: Methods of vesicle production and quantification can influence biological and experimental outcomes. Microbiology Spectrum, 9, e0127321.
Blijdorp, C. J., Tutakhel, O. A. Z., Hartjes, T. A., van den Bosch, T. P. P., van Heugten, M. H., Rigalli, J. P., Willemsen, R., Musterd-Bhaggoe, U. M., Barros, E. R., Carles-Fontana, R., Carvajal, C. A., Arntz, O. J., van de Loo, F. A. J., Jenster, G., Clahsen-van Groningen, M. C., Cuevas, C. A., Severs, D., Fenton, R. A., van Royen, M. E., … Hoorn, E. J. (2021). Comparing approaches to normalize, quantify, and characterize urinary extracellular vesicles. Journal of the American Society of Nephrology, 32, 1210–1226.
Boere, J., van de Lest, C. H. A., de Grauw, J. C., Plomp, S. G. M., Libregts, S., Arkesteijn, G. J. A., Malda, J., Wauben, M. H. M., & van Weeren, P. R. (2019). Extracellular vesicles in synovial fluid from juvenile horses: No age-related changes in the quantitative profile. Veterinary Journal, 244, 91–93.
Boing, A. N., van der Pol, E., Grootemaat, A. E., Coumans, F. A., Sturk, A., & Nieuwland, R. (2014). Single-step isolation of extracellular vesicles by size-exclusion chromatography. Journal of Extracellular Vesicles, 3, 00–00.
Bonsergent, E., Grisard, E., Buchrieser, J., Schwartz, O., Théry, C., & Lavieu, G. (2021). Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells. Nature Communications, 12, 1864.
Bonsergent, E., & Lavieu, G. (2019). Content release of extracellular vesicles in a cell-free extract. Febs Letters, 593, 1983–1992.
Bordanaba-Florit, G., Royo, F., Kruglik, S. G., & Falcón-Pérez, J. M. (2021). Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles. Nature Protocols, 16, 3163–3185.
Borghesan, M., Fafián-Labora, J., Eleftheriadou, O., Carpintero-Fernández, P., Paez-Ribes, M., Vizcay-Barrena, G., Swisa, A., Kolodkin-Gal, D., Ximénez-Embún, P., Lowe, R., Martín-Martín, B., Peinado, H., Muñoz, J., Fleck, R. A., Dor, Y., Ben-Porath, I., Vossenkamper, A., Muñoz-Espin, D., & O'Loghlen, A. (2019). Small extracellular vesicles are key regulators of non-cell autonomous intercellular communication in senescence via the interferon protein IFITM3. Cell Reports, 27, 3956–3971.e6.
Bortot, B., Apollonio, M., Rampazzo, E., Valle, F., Brucale, M., Ridolfi, A., Ura, B., Addobbati, R., Di Lorenzo, G., Romano, F., Buonomo, F., Ripepi, C., Ricci, G., & Biffi, S. (2021). Small extracellular vesicles from malignant ascites of patients with advanced ovarian cancer provide insights into the dynamics of the extracellular matrix. Molecular Oncology, 15, 3596–3614.
Bose, S., Aggarwal, S., Singh, D. V., & Acharya, N. (2020). Extracellular vesicles: An emerging platform in gram-positive bacteria. Microbial Cell, 7, 312–322.
Botha, J., Handberg, A., & Simonsen, J. B. (2022). Lipid-based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis. Journal of Extracellular Vesicles, 11, e12200.
Bracht, J. W. P., Los, M., van Eijndhoven, M. A. J., Bettin, B., van der Pol, E., Pegtel, D. M., & Nieuwland, R. (2023). Platelet removal from human blood plasma improves detection of extracellular vesicle-associated miRNA. Journal of Extracellular Vesicles, 12, e12302.
Brown, L., Wolf, J. M., Prados-Rosales, R., & Casadevall, A. (2015). Through the wall: Extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi. Nature Reviews Microbiology, 13, 620–630.
Budnik, V., Ruiz-Cañada, C., & Wendler, F. (2016). Extracellular vesicles round off communication in the nervous system. Nature Reviews Neuroscience, 17, 160–172.
Buntsma, N. C., Gąsecka, A., Roos, Y., van Leeuwen, T. G., van der Pol, E., & Nieuwland, R. (2022). EDTA stabilizes the concentration of platelet-derived extracellular vesicles during blood collection and handling. Platelets, 33, 764–771.
Burger, D., Thibodeau, J. F., Holterman, C. E., Burns, K. D., Touyz, R. M., & Kennedy, C. R. (2014). Urinary podocyte microparticles identify prealbuminuric diabetic glomerular injury. Journal of the American Society of Nephrology, 25, 1401–1407.
Busatto, S., Vilanilam, G., Ticer, T., Lin, W. L., Dickson, D. W., Shapiro, S., Bergese, P., & Wolfram, J. (2018). Tangential flow filtration for highly efficient concentration of extracellular vesicles from large volumes of fluid. Cells, 7(12), 273.
Busatto, S., Yang, Y., Iannotta, D., Davidovich, I., Talmon, Y., & Wolfram, J. (2022). Considerations for extracellular vesicle and lipoprotein interactions in cell culture assays. Journal of Extracellular Vesicles, 11, e12202.
Busatto, S., Zendrini, A., Radeghieri, A., Paolini, L., Romano, M., Presta, M., & Bergese, P. (2019). The nanostructured secretome. Biomaterials Science, 8, 39–63.
Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., & Wittwer, C. T. (2009). The MIQE Guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry, 55, 611–622.
Butler, H. J., Ashton, L., Bird, B., Cinque, G., Curtis, K., Dorney, J., Esmonde-White, K., Fullwood, N. J., Gardner, B., Martin-Hirsch, P. L., Walsh, M. J., McAinsh, M. R., Stone, N., & Martin, F. L. (2016). Using Raman spectroscopy to characterize biological materials. Nature Protocols, 11, 664–687.
Buzas, E. I. (2022). Opportunities and challenges in studying the extracellular vesicle corona. Nature Cell Biology, 24, 1322–1325.
Cai, J., Han, Y., Ren, H., Chen, C., He, D., Zhou, L., Eisner, G. M., Asico, L. D., Jose, P. A., & Zeng, C. (2013). Extracellular vesicle-mediated transfer of donor genomic DNA to recipient cells is a novel mechanism for genetic influence between cells. Journal of Molecular Cell Biology, 5, 227–238.
Calò, A., Reguera, D., Oncins, G., Persuy, M. A., Sanz, G., Lobasso, S., Corcelli, A., Pajot-Augy, E., & Gomila, G. (2014). Force measurements on natural membrane nanovesicles reveal a composition-independent, high Young's modulus. Nanoscale, 6, 2275–2285.
Cambier, L., Stachelek, K., Triska, M., Jubran, R., Huang, M., Li, W., Zhang, J., Li, J., & Cobrinik, D. (2021). Extracellular vesicle-associated repetitive element DNAs as candidate osteosarcoma biomarkers. Scientific Reports, 11, 94.
Cameron, J. M., Butler, H. J., Palmer, D. S., & Baker, M. J. (2018). Biofluid spectroscopic disease diagnostics: A review on the processes and spectral impact of drying. Journal of Biophotonics, 11, e201700299.
Cameron, S., Gillio-Meina, C., Ranger, A., Choong, K., & Fraser, D. D. (2019). Collection and analyses of cerebrospinal fluid for pediatric translational research. Pediatric Neurology, 98, 3–17.
Carlomagno, C., Giannasi, C., Niada, S., Bedoni, M., Gualerzi, A., & Brini, A. T. (2021). Raman fingerprint of extracellular vesicles and conditioned media for the reproducibility assessment of cell-free therapeutics. Frontiers in Bioengineering and Biotechnology, 9, 640617.
Carrel, A., & Burrows, M. T. (1911). Cultivation of tissues in vitro and its technique. Journal of Experimental Medicine, 13, 387–396.
Carreras-Planella, L., Cucchiari, D., Cañas, L., Juega, J., Franquesa, M., Bonet, J., Revuelta, I., Diekmann, F., Taco, O., Lauzurica, R., & Borràs, F. E. (2021). Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts. Journal of Nephrology, 34, 861–874.
Catalano, M., & O'Driscoll, L. (2020). Inhibiting extracellular vesicles formation and release: A review of EV inhibitors. Journal of Extracellular Vesicles, 9, 1703244.
Cavallaro, S., Pevere, F., Stridfeldt, F., Görgens, A., Paba, C., Sahu, S. S., Mamand, D. R., Gupta, D., El Andaloussi, S., Linnros, J., & Dev, A. (2021). Multiparametric profiling of single nanoscale extracellular vesicles by combined atomic force and fluorescence microscopy: Correlation and heterogeneity in their molecular and biophysical features. Small, 17, e2008155.
Cavallaro, S., Hååg, P., Viktorsson, K., Krozer, A., Fogel, K., Lewensohn, R., Linnros, J., & Dev, A. (2021). Comparison and optimization of nanoscale extracellular vesicle imaging by scanning electron microscopy for accurate size-based profiling and morphological analysis. Nanoscale Advances, 3(11), 3053–3063.
Champagne-Jorgensen, K., Mian, M. F., McVey Neufeld, K. A., Stanisz, A. M., & Bienenstock, J. (2021). Membrane vesicles of Lacticaseibacillus rhamnosus JB-1 contain immunomodulatory lipoteichoic acid and are endocytosed by intestinal epithelial cells. Scientific Reports, 11, 13756.
Chen, C., Zong, S., Wang, Z., Lu, J., Zhu, D., Zhang, Y., & Cui, Y. (2016). Imaging and intracellular tracking of cancer-derived exosomes using single-molecule localization-based super-resolution microscope. ACS Applied Materials & Interfaces, 8, 25825–25833.
Chen, C., Zong, S., Wang, Z., Lu, J., Zhu, D., Zhang, Y., Zhang, R., & Cui, Y. (2018). Visualization and intracellular dynamic tracking of exosomes and exosomal miRNAs using single molecule localization microscopy. Nanoscale, 10, 5154–5162.
Chernyshev, V. S., Rachamadugu, R., Tseng, Y. H., Belnap, D. M., Jia, Y., Branch, K. J., Butterfield, A. E., Pease, L. F., Bernard, P. S., & Skliar, M. (2015). Size and shape characterization of hydrated and desiccated exosomes. Analytical and Bioanalytical Chemistry, 407, 3285–3301.
Chiappin, S., Antonelli, G., Gatti, R., & De Palo, E. F. (2007). Saliva specimen: A new laboratory tool for diagnostic and basic investigation. Clinica Chimica Acta, 383, 30–40.
Christianson, H. C., Svensson, K. J., van Kuppevelt, T. H., Li, J. P., & Belting, M. (2013). Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. PNAS, 110, 17380–17385.
Chuo, S. T., Chien, J. C., & Lai, C. P. (2018). Imaging extracellular vesicles: Current and emerging methods. Journal of Biomedical Science, 25, 91.
Churchman, L. S, & Spudich, J. A. (2012). Colocalization of fluorescent probes: Accurate and precise registration with nanometer resolution. Cold Spring Harbor Protocols, 2012, 141–149.
Cianciaruso, C., Beltraminelli, T., Duval, F., Nassiri, S., Hamelin, R., Mozes, A., Gallart-Ayala, H., Ceada Torres, G., Torchia, B., Ries, C. H., Ivanisevic, J., & De Palma, M. (2019). Molecular profiling and functional analysis of macrophage-derived tumor extracellular vesicles. Cell Reports, 27, 3062–3080.e11.
Cimorelli, M., Nieuwland, R., Varga, Z., & van der Pol, E. (2021). Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing. PLoS ONE, 16, e0249603.
Ciullo, A., Li, C., Li, L., Ungerleider, K. C., Peck, K., Marbán, E., & Ibrahim, A. G. E. (2022). Biodistribution of unmodified cardiosphere-derived cell extracellular vesicles using single RNA tracing. Journal of Extracellular Vesicles, 11, e12178.
Clancy, J. W., Sheehan, C. S., Boomgarden, A. C., & D'Souza-Schorey, C. (2022). Recruitment of DNA to tumor-derived microvesicles. Cell Reports, 38, 110443.
Clayton, A., Boilard, E., Buzas, E. I., Cheng, L., Falcón-Perez, J. M., Gardiner, C., Gustafson, D., Gualerzi, A., Hendrix, A., Hoffman, A., Jones, J., Lässer, C., Lawson, C., Lenassi, M., Nazarenko, I., O'Driscoll, L., Pink, R., Siljander, P. R., Soekmadji, C., … Nieuwland, R. (2019). Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles. Journal of Extracellular Vesicles, 8, 1647027.
Clayton, A., Buschmann, D., Byrd, J. B., Carter, D. R. F., Cheng, L., Compton, C., Daaboul, G., Devitt, A., Falcon-Perez, J. M., Gardiner, C., Gustafson, D., Harrison, P., Helmbrecht, C., Hendrix, A., Hill, A., Hoffman, A., Jones, J. C., Kalluri, R., Kang, J. Y., … Nieuwland, R. (2018). Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017. Journal of Extracellular Vesicles, 7, 1473707.
Clupper, M., Gill, R., Elsayyid, M., Touroutine, D., Caplan, J. L., & Tanis, J. E. (2022). Kinesin-2 motors differentially impact biogenesis of extracellular vesicle subpopulations shed from sensory cilia. Iscience, 25, 105262.
Coffman, V. C., & Wu, J. Q. (2014). Every laboratory with a fluorescence microscope should consider counting molecules. Molecular Biology of the Cell, 25, 1545–1548.
Colombo, F., Norton, E. G., & Cocucci, E. (2021). Microscopy approaches to study extracellular vesicles. Biochimica et Biophysica Acta (BBA) - General Subjects, 1865, 129752.
Correll, V. L., Otto, J. J., Risi, C. M., Main, B. P., Boutros, P. C., Kislinger, T., Galkin, V. E., Nyalwidhe, J. O., Semmes, O. J., & Yang, L. (2022). Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis. Journal of Extracellular Vesicles, 11, e12184.
Corrigan, L., Redhai, S., Leiblich, A., Fan, S. J., Perera, S. M., Patel, R., Gandy, C., Wainwright, S. M., Morris, J. F., Hamdy, F., Goberdhan, D. C., & Wilson, C. (2014). BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior. Journal of Cell Biology, 206, 671–688.
Corso, G., Heusermann, W., Trojer, D., Görgens, A., Steib, E., Voshol, J., Graff, A., Genoud, C., Lee, Y., Hean, J., Nordin, J. Z., Wiklander, O. P. B., El Andaloussi, S., & Meisner-Kober, N. (2019). Systematic characterization of extracellular vesicle sorting domains and quantification at the single molecule – single vesicle level by fluorescence correlation spectroscopy and single particle imaging. Journal of Extracellular Vesicles, 8, 1663043.
Costa-Silva, B., Aiello, N. M., Ocean, A. J., Singh, S., Zhang, H., Thakur, B. K., Becker, A., Hoshino, A., Mark, M. T., Molina, H., Xiang, J., Zhang, T., Theilen, T. M., García-Santos, G., Williams, C., Ararso, Y., Huang, Y., Rodrigues, G., Shen, T. L., … Lyden, D. (2015). Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver. Nature Cell Biology, 17, 816–826.
Coumans, F. A., van der Pol, E., Boing, A. N., Hajji, N., Sturk, G., van Leeuwen, T. G., & Nieuwland, R. (2014). Reproducible extracellular vesicle size and concentration determination with tunable resistive pulse sensing. Journal of Extracellular Vesicles, 3, 25922.
Coumans, F. A. W., Brisson, A. R., Buzas, E. I., Dignat-George, F., Drees, E. E. E., El-Andaloussi, S., Emanueli, C., Gasecka, A., Hendrix, A., Hill, A. F., Lacroix, R., Lee, Y., van Leeuwen, T. G., Mackman, N., Mager, I., Nolan, J. P., van der Pol, E., Pegtel, D. M., Sahoo, S., … Nieuwland, R. (2017). Methodological guidelines to study extracellular vesicles. Circulation Research, 120, 1632–1648.
Crescitelli, R., Lässer, C., Jang, S. C., Cvjetkovic, A., Malmhäll, C., Karimi, N., Höög, J. L., Johansson, I., Fuchs, J., Thorsell, A., Gho, Y. S., Bagge, R. O, & Lötvall, J. (2020). Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles, 9, 1722433.
Crescitelli, R., Lasser, C., & Lotvall, J. (2021). Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature Protocols, 16, 1548–1580.
Crescitelli, R., Lässer, C., Szabó, T. G., Kittel, A., Eldh, M., Dianzani, I., Buzás, E. I., & Lötvall, J. (2013). Distinct RNA profiles in subpopulations of extracellular vesicles: Apoptotic bodies, microvesicles and exosomes. Journal of Extracellular Vesicles, 2, 20677.
Cvjetkovic, A., Jang, S. C., Konečná, B., Höög, J. L., Sihlbom, C., Lässer, C., & Lötvall, J. (2016). Detailed analysis of protein topology of extracellular vesicles – Evidence of unconventional membrane protein orientation. Scientific Reports, 6, 36338.
Czarniak, N., Kamińska, J., Matowicka-Karna, J., & Koper-Lenkiewicz, O. M. (2023). Cerebrospinal fluid-basic concepts review. Biomedicines, 11, 1461.
Daaboul, G. G., Gagni, P., Benussi, L., Bettotti, P., Ciani, M., Cretich, M., Freedman, D. S., Ghidoni, R., Ozkumur, A. Y., Piotto, C., Prosperi, D., Santini, B., Unlu, M. S., & Chiari, M. (2016). Digital detection of exosomes by interferometric imaging. Scientific Reports, 6, 37246.
Das, S., Ansel, K. M., Bitzer, M., Breakefield, X. O., Charest, A., Galas, D. J., Gerstein, M. B., Gupta, M., Milosavljevic, A., McManus, M. T., Patel, T., Raffai, R. L., Rozowsky, J., Roth, M. E., Saugstad, J. A., Van Keuren-Jensen, K., Weaver, A. M., & Laurent, L. C. (2019). The extracellular RNA communication consortium: Establishing foundational knowledge and technologies for extracellular RNA research. Cell, 177, 231–242.
Singorenko, D. P., Chang, V., Whitcombe, A., Simonov, D., Hong, J., Phillips, A., Swift, S., & Blenkiron, C. (2017). Isolation of membrane vesicles from prokaryotes: A technical and biological comparison reveals heterogeneity. Journal of Extracellular Vesicles, 6, 1324731.
de Jong, O. G., Murphy, D. E., Mäger, I., Willms, E., Garcia-Guerra, A., Gitz-Francois, J. J., Lefferts, J., Gupta, D., Steenbeek, S. C., van Rheenen, J., El Andaloussi, S., Schiffelers, R. M., Wood, M. J. A., & Vader, P. (2020). A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. Nature Communications, 11, 1113.
de Rond, L., Coumans, F. A. W., Nieuwland, R., van Leeuwen, T. G., & van der Pol, E. (2018). Deriving extracellular vesicle size from scatter intensities measured by flow cytometry. Current Protocols in Cytometry, 86, e43.
de Rond, L., van der Pol, E., Hau, C. M., Varga, Z., Sturk, A., van Leeuwen, T. G., Nieuwland, R., & Coumans, F. A. W. (2018). Comparison of generic fluorescent markers for detection of extracellular vesicles by flow cytometry. Clinical Chemistry, 64, 680–689.
de Voogt, W. S., Tanenbaum, M. E., & Vader, P. (2021). Illuminating RNA trafficking and functional delivery by extracellular vesicles. Advanced Drug Delivery Reviews, 174, 250–264.
de Vrij, J., Maas, S. L., van Nispen, M., Sena-Esteves, M., Limpens, R. W., Koster, A. J., Leenstra, S., Lamfers, M. L., & Broekman, M. L. (2013). Quantification of nanosized extracellular membrane vesicles with scanning ion occlusion sensing. Nanomedicine (Lond), 8, 1443–1458.
Dhondt, B., Geeurickx, E., Tulkens, J., Van Deun, J., Vergauwen, G., Lippens, L., Miinalainen, I., Rappu, P., Heino, J., Ost, P., Lumen, N., De Wever, O., & Hendrix, A. (2020). Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine. Journal of Extracellular Vesicles, 9, 1736935.
Dhondt, B., Pinheiro, C., Geeurickx, E., Tulkens, J., Vergauwen, G., Van Der Pol, E., Nieuwland, R., Decock, A., Miinalainen, I., Rappu, P., Schroth, G., Kuersten, S., Vandesompele, J., Mestdagh, P., Lumen, N., De Wever, O., & Hendrix, A. (2023). Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics. Journal of Extracellular Vesicles, 12, e12315.
Dixson, A. C., Dawson, T. R., Di Vizio, D., & Weaver, A. M. (2023). Context-specific regulation of extracellular vesicle biogenesis and cargo selection. Nature Reviews Molecular Cell Biology, 24, 454–476.
Dogrammatzis, C., Saleh, S., Deighan, C., & Kalamvoki, M. (2021). Diverse populations of extracellular vesicles with opposite functions during herpes simplex virus 1 infection. Journal of Virology, 95(6), e02357–20.
Dong, L., Zieren, R. C., Horie, K., Kim, C. J., Mallick, E., Jing, Y., Feng, M., Kuczler, M. D., Green, J., Amend, S. R., Witwer, K. W., de Reijke, T. M., Cho, Y. K., Pienta, K. J., & Xue, W. (2020). Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium. Journal of Extracellular Vesicles, 10, e12044.
Dooley, K., McConnell, R. E., Xu, K., Lewis, N. D., Haupt, S., Youniss, M. R., Martin, S., Sia, C. L., McCoy, C., Moniz, R. J., Burenkova, O., Sanchez-Salazar, J., Jang, S. C., Choi, B., Harrison, R. A., Houde, D., Burzyn, D., Leng, C., Kirwin, K., … Williams, D. E. (2021). A versatile platform for generating engineered extracellular vesicles with defined therapeutic properties. Molecular Therapy, 29, 1729–1743.
Driedonks, T. A. P., Nijen Twilhaar, M. K., & Nolte-’t Hoen, E. N. M. (2019). Technical approaches to reduce interference of fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells. Journal of Extracellular Vesicles, 8(1), 1552059.
Driedonks, T., Jiang, L., Carlson, B., Han, Z., Liu, G., Queen, S. E., Shirk, E. N., Gololobova, O., Liao, Z., Nyberg, L. H., Lima, G., Paniushkina, L., Garcia-Contreras, M., Schonvisky, K., Castell, N., Stover, M., Guerrero-Martin, S., Richardson, R., Smith, B., … Witwer, K. W. (2022). Pharmacokinetics and biodistribution of extracellular vesicles administered intravenously and intranasally to Macaca nemestrina. Journal of Extracellular Biology, 1, e59.
Duijvesz, D., Versluis, C. Y., van der Fels, C. A., Vredenbregt-van den Berg, M. S., Leivo, J., Peltola, M. T., Bangma, C. H., Pettersson, K. S., & Jenster, G. (2015). Immuno-based detection of extracellular vesicles in urine as diagnostic marker for prostate cancer. International Journal of Cancer, 137, 2869–2878.
Eldh, M., Lötvall, J., Malmhäll, C., & Ekström, K. (2012). Importance of RNA isolation methods for analysis of exosomal RNA: Evaluation of different methods. Molecular Immunology, 50, 278–286.
Elgamal, S., Colombo, F., Cottini, F., Byrd, J. C., & Cocucci, E. (2020). Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light-sheet fluorescence microscopy. Methods in Enzymology, 645, 79–107.
Enciso-Martinez, A., van der Pol, E., Lenferink, A. T. M., Terstappen, L., van Leeuwen, T. G., & Otto, C. (2020). Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles. Nanomedicine, 24, 102109.
Erdbrügger, U., Blijdorp, C. J., Bijnsdorp, I. V., Borràs, F. E., Burger, D., Bussolati, B., Byrd, J. B., Clayton, A., Dear, J. W., Falcón-Pérez, J. M., Grange, C., Hill, A. F., Holthöfer, H., Hoorn, E. J., Jenster, G., Jimenez, C. R., Junker, K., Klein, J., Knepper, M. A., … Martens-Uzunova, E. S. (2021). Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles. Journal of Extracellular Vesicles, 10, e12093.
Estrada, A. L., Valenti, Z. J., Hehn, G., Amorese, A. J., Williams, N. S., Balestrieri, N. P., Deighan, C., Allen, C. P., Spangenburg, E. E., Kruh-Garcia, N. A., & Lark, D. S. (2022). Extracellular vesicle secretion is tissue-dependent ex vivo and skeletal muscle myofiber extracellular vesicles reach the circulation in vivo. American Journal of Physiology. Cell Physiology, 322, C246–C259.
EV-TRACK Consortium, van Deun, J., Mestdagh, P., Agostinis, P., Akay, O., Anand, S., Anckaert, J., Martinez, Z. A., Baetens, T., Beghein, E., Bertier, L., Berx, G., Boere, J., Boukouris, S., Bremer, M., Buschmann, D., Byrd, J. B., Casert, C., Cheng, L., … Hendrix, A. (2017). EV-TRACK: Transparent reporting and centralizing knowledge in extracellular vesicle research. Nature Methods, 14, 228–232.
Evtushenko, E. G., Bagrov, D. V., Lazarev, V. N., Livshits, M. A., & Khomyakova, E. (2020). Adsorption of extracellular vesicles onto the tube walls during storage in solution. PLoS ONE, 15, e0243738.
Fan, S. J., Kroeger, B., Marie, P. P., Bridges, E. M., Mason, J. D., McCormick, K., Zois, C. E., Sheldon, H., Khalid Alham, N., Johnson, E., Ellis, M., Stefana, M. I., Mendes, C. C., Wainwright, S. M., Cunningham, C., Hamdy, F. C., Morris, J. F., Harris, A. L., Wilson, C., & Goberdhan, D. C. (2020). Glutamine deprivation alters the origin and function of cancer cell exosomes. Embo Journal, 39, e103009.
Feng, D., Zhao, W. L., Ye, Y. Y., Bai, X. C., Liu, R. Q., Chang, L. F., Zhou, Q., & Sui, S. F. (2010). Cellular internalization of exosomes occurs through phagocytosis. Traffic (Copenhagen, Denmark), 11, 675–687.
Foers, A. D., Chatfield, S., Dagley, L. F., Scicluna, B. J., Webb, A. I., Cheng, L., Hill, A. F., Wicks, I. P., & Pang, K. C. (2018). Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography. Journal of Extracellular Vesicles, 7, 1490145.
Foers, A. D., Dagley, L. F., Chatfield, S., Webb, A. I., Cheng, L., Hill, A. F., Wicks, I. P., & Pang, K. C. (2020). Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid. Clinical & Translational Immunology, 9, e1185.
Gaetani, L., Blennow, K., Calabresi, P., Di Filippo, M., Parnetti, L., & Zetterberg, H. (2019). Neurofilament light chain as a biomarker in neurological disorders. Journal of Neurology, Neurosurgery, and Psychiatry, 90, 870–881.
Gallart-Palau, X., Serra, A., & Sze, S. K. (2016). Enrichment of extracellular vesicles from tissues of the central nervous system by PROSPR. Molecular Neurodegeneration, 11, 41.
Gallego-Perez, D., Chang, L., Shi, J., Ma, J., Kim, S. H., Zhao, X., Malkoc, V., Wang, X., Minata, M., Kwak, K. J., Wu, Y., Lafyatis, G. P., Lu, W., Hansford, D. J., Nakano, I., & Lee, L. J. (2016). On-chip clonal analysis of glioma-stem-cell motility and therapy resistance. Nano Letters, 16, 5326–5332.
Gámez-Valero, A., Monguió-Tortajada, M., Carreras-Planella, L., Franquesa, M. I., Beyer, K., & Borràs, F. E. (2016). Size-exclusion chromatography-based isolation minimally alters extracellular vesicles’ characteristics compared to precipitating agents. Scientific Reports, 6, 33641.
Gandham, S., Su, X., Wood, J., Nocera, A L., Chandra Alli, S., Milane, L., Zimmerman, A., Amiji, M., & Ivanov, A R. (2020). Technologies and standardization in research on extracellular vesicles. Trends in Biotechnology, 38(10), 1066–1098.
Gao, H. N., Guo, H. Y., Zhang, H., Xie, X. L., Wen, P. C., & Ren, F. Z. (2019). Yak-milk-derived exosomes promote proliferation of intestinal epithelial cells in an hypoxic environment. Journal of Dairy Science, 102, 985–996.
Gao, K., Zhu, W., Li, H., Ma, D., Liu, W., Yu, W., Wang, L., Cao, Y., & Jiang, Y. (2020). Association between cytokines and exosomes in synovial fluid of individuals with knee osteoarthritis. Modern Rheumatology, 30, 758–764.
Gao, X., Ran, N., Dong, X., Zuo, B., Yang, R., Zhou, Q., Moulton, H. M., Seow, Y., & Yin, H. (2018). Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy. Science Translational Medicine, 10(444), eaat0195.
García-Silva, S., Benito-Martín, A., Nogués, L., Hernández-Barranco, A., Mazariegos, M. S., Santos, V., Hergueta-Redondo, M., Ximénez-Embún, P., Kataru, R. P., Lopez, A. A., Merino, C., Sánchez-Redondo, S., Graña-Castro, O., Matei, I., Nicolás-Avila, J. Á., Torres-Ruiz, R., Rodríguez-Perales, S., Martínez, L., Pérez-Martínez, M., … Peinado, H. (2021). Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism. Nature Cancer, 2, 1387–1405.
García-Silva, S., Benito-Martín, A., Sánchez-Redondo, S., Hernández-Barranco, A., Ximénez-Embún, P., Nogués, L., Mazariegos, M. S., Brinkmann, K., Amor López, A., Meyer, L., Rodríguez, C., García-Martín, C., Boskovic, J., Letón, R., Montero, C., Robledo, M., Santambrogio, L., Sue Brady, M., Szumera-Ciećkiewicz, A., … Peinado, H. (2019). Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF (V600E) mutation. Journal of Experimental Medicine, 216, 1061–1070.
Gardiner, C., Di Vizio, D., Sahoo, S., Théry, C., Witwer, K. W., Wauben, M., & Hill, A. F. (2016). Techniques used for the isolation and characterization of extracellular vesicles: Results of a worldwide survey. Journal of Extracellular Vesicles, 5, 32945.
Gardiner, C., Shaw, M., Hole, P., Smith, J., Tannetta, D., Redman, C. W., & Sargent, I. L. (2014). Measurement of refractive index by nanoparticle tracking analysis reveals heterogeneity in extracellular vesicles. Journal of Extracellular Vesicles, 3, 25361.
Gaurivaud, P., Ganter, S., Villard, A., Manso-Silvan, L., Chevret, D., Boulé, C., Monnet, V., & Tardy, F. (2018). Mycoplasmas are no exception to extracellular vesicles release: Revisiting old concepts. PLoS ONE, 13, e0208160.
Gautron, J., Stapane, L., Roy, N. L., Nys, Y., Rodriguez-Navarro, A. B., & Hincke, M. T. (2021). Avian eggshell biomineralization: An update on its structure, mineralogy and protein tool kit. BMC Molecular and Cell Biology, 22, 11.
Geeurickx, E., Tulkens, J., Dhondt, B., Van Deun, J., Lippens, L., Vergauwen, G., Heyrman, E., De Sutter, D., Gevaert, K., Impens, F., Miinalainen, I., Van Bockstal, P. J., De Beer, T., Wauben, M. H. M., Nolte-’t-Hoen, E. N. M., Bloch, K., Swinnen, J. V., van der Pol, E., Nieuwland, R., … Hendrix, A. (2019). The generation and use of recombinant extracellular vesicles as biological reference material. Nature Communications, 10, 3288.
Gelibter, S., Marostica, G., Mandelli, A., Siciliani, S., Podini, P., Finardi, A., & Furlan, R. (2022). The impact of storage on extracellular vesicles: A systematic study. Journal of Extracellular Vesicles, 11, e12162.
Ghoroghi, S., Mary, B., Larnicol, A., Asokan, N., Klein, A., Osmani, N., Busnelli, I., Delalande, F., Paul, N., Halary, S., Gros, F., Fouillen, L., Haeberle, A. M., Royer, C., Spiegelhalter, C., André-Grégoire, G., Mittelheisser, V., Detappe, A., Murphy, K., … Hyenne, V. (2021). Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes. Elife, 10, e61539.
Giddings, J. C., Yang, F. J., & Myers, M. N. (1976). Flow-field-flow fractionation: A versatile new separation method. Science, 193, 1244–1245.
Gimona, M., Brizzi, M. F., Choo, A. B. H., Dominici, M., Davidson, S. M., Grillari, J., Hermann, D. M., Hill, A. F., de Kleijn, D., Lai, R. C., Lai, C. P., Lim, R., Monguió-Tortajada, M., Muraca, M., Ochiya, T., Ortiz, L. A., Toh, W. S., Yi, Y. W., Witwer, K. W., … Lim, S. K. (2021). Critical considerations for the development of potency tests for therapeutic applications of mesenchymal stromal cell-derived small extracellular vesicles. Cytotherapy, 23, 373–380.
Gobbo, J., Marcion, G., Cordonnier, M., Dias, A. M. M., Pernet, N., Hammann, A., Richaud, S., Mjahed, H., Isambert, N., Clausse, V., Rébé, C., Bertaut, A., Goussot, V., Lirussi, F., Ghiringhelli, F., de Thonel, A., Fumoleau, P., Seigneuric, R., & Garrido, C. (2016). Restoring anticancer immune response by targeting tumor-derived exosomes with a HSP70 peptide aptamer. JNCI: Journal of the National Cancer Institute, 108, 00–00.
Gomar-Vercher, S., Simón-Soro, A., Montiel-Company, J. M., Almerich-Silla, J. M., & Mira, A. (2018). Stimulated and unstimulated saliva samples have significantly different bacterial profiles. PLoS ONE, 13, e0198021.
Gomes, D. E., & Witwer, K. W. (2022). L1CAM-associated extracellular vesicles: A systematic review of nomenclature, sources, separation, and characterization. Journal of Extracellular Biology, 1(3), e35.
Gorgens, A., Bremer, M., Ferrer-Tur, R., Murke, F., Tertel, T., Horn, P. A., Thalmann, S., Welsh, J. A., Probst, C., Guerin, C., Boulanger, C. M., Jones, J. C., Hanenberg, H., Erdbrugger, U., Lannigan, J., Ricklefs, F. L., El-Andaloussi, S., & Giebel, B. (2019). Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material. Journal of Extracellular Vesicles, 8, 1587567.
Görgens, A., Corso, G., Hagey, D. W., Jawad Wiklander, R., Gustafsson, M. O., Felldin, U., Lee, Y., Bostancioglu, R. B., Sork, H., Liang, X., Zheng, W., Mohammad, D. K., van de Wakker, S. I., Vader, P., Zickler, A. M., Mamand, D. R., Ma, L., Holme, M. N., Stevens, M. M., … El Andaloussi, S. (2022). Identification of storage conditions stabilizing extracellular vesicles preparations. Journal of Extracellular Vesicles, 11, e12238.
Gori, A., Romanato, A., Greta, B., Strada, A., Gagni, P., Frigerio, R., Brambilla, D., Vago, R., Galbiati, S., Picciolini, S., Bedoni, M., Daaboul, G. G., Chiari, M., & Cretich, M. (2020). Membrane-binding peptides for extracellular vesicles on-chip analysis. Journal of Extracellular Vesicles, 9, 1751428.
Gould, T. J., Hess, S. T., & Bewersdorf, J. (2012). Optical nanoscopy: From acquisition to analysis. Annual Review of Biomedical Engineering, 14, 231–254.
Gradilla, A. C., González, E., Seijo, I., Andrés, G., Bischoff, M., González-Mendez, L., Sánchez, V., Callejo, A., Ibáñez, C., Guerra, M., Ortigão-Farias, J. R., Sutherland, J. D., González, M., Barrio, R., Falcón-Pérez, J. M., & Guerrero, I. (2014). Exosomes as Hedgehog carriers in cytoneme-mediated transport and secretion. Nature Communications, 5, 5649.
Granvogl, B., Ploscher, M., & Eichacker, L. A. (2007). Sample preparation by in-gel digestion for mass spectrometry-based proteomics. Analytical and Bioanalytical Chemistry, 389, 991–1002.
Gross, J. C., Chaudhary, V., Bartscherer, K., & Boutros, M. (2012). Active Wnt proteins are secreted on exosomes. Nature Cell Biology, 14, 1036–1045.
Gualerzi, A., Kooijmans, S. A. A., Niada, S., Picciolini, S., Brini, A. T., Camussi, G., & Bedoni, M. (2019). Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality. Journal of Extracellular Vesicles, 8, 1568780.
Gualerzi, A., Niada, S., Giannasi, C., Picciolini, S., Morasso, C., Vanna, R., Rossella, V., Masserini, M., Bedoni, M., Ciceri, F., Bernardo, M. E., Brini, A. T., & Gramatica, F. (2017). Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells. Scientific Reports, 7, 9820.
Gupta, D., Liang, X., Pavlova, S., Wiklander, O. P. B., Corso, G., Zhao, Y., Saher, O., Bost, J., Zickler, A. M., Piffko, A., Maire, C. L., Ricklefs, F. L., Gustafsson, O., Llorente, V. C., Gustafsson, M. O., Bostancioglu, R. B., Mamand, D. R., Hagey, D. W., Görgens, A., … El Andaloussi, S. (2020). Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging. Journal of Extracellular Vesicles, 9, 1800222.
Gyorgy, B., Modos, K., Pallinger, E., Paloczi, K., Pasztoi, M., Misjak, P., Deli, M. A., Sipos, A., Szalai, A., Voszka, I., Polgar, A., Toth, K., Csete, M., Nagy, G., Gay, S., Falus, A., Kittel, A., & Buzas, E. I. (2011). Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood, 117, e39–e48.
Gyorgy, B., Paloczi, K., Kovacs, A., Barabas, E., Beko, G., Varnai, K., Pallinger, E., Szabo-Taylor, K., Szabo, T. G., Kiss, A. A., Falus, A., & Buzas, E. I. (2014). Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube. Thrombosis Research, 133, 285–292.
Hackley, V. A., & Clogston, J. D. (2011). Measuring the hydrodynamic size of nanoparticles in aqueous media using batch-mode dynamic light scattering. Methods in Molecular Biology, 697, 35–52.
Han, C., Kang, H., Yi, J., Kang, M., Lee, H., Kwon, Y., Jung, J., Lee, J., & Park, J. (2021). Single-vesicle imaging and co-localization analysis for tetraspanin profiling of individual extracellular vesicles. Journal of Extracellular Vesicles, 10(3), e12047.
Han, P., Bartold, P. M., Salomon, C., & Ivanovski, S. (2021). Salivary outer membrane vesicles and DNA methylation of small extracellular vesicles as biomarkers for periodontal status: A pilot study. International Journal of Molecular Sciences, 22, 2423.
He, B., Cai, Q., Qiao, L., Huang, C. Y., Wang, S., Miao, W., Ha, T., Wang, Y., & Jin, H. (2021). RNA-binding proteins contribute to small RNA loading in plant extracellular vesicles. Nature Plants, 7, 342–352.
Hebisch, E., Wagner, E., Westphal, V., Sieber, J. J., & Lehnart, S. E. (2017). A protocol for registration and correction of multicolour STED superresolution images. Journal of Microscopy, 267, 160–175.
Heddleston, J. M., Aaron, J. S., Khuon, S., & Chew, T. L. (2021). A guide to accurate reporting in digital image acquisition – can anyone replicate your microscopy data? Journal of Cell Science, 134(6), jcs254144.
Hegyesi, H., Pallinger, É., Mecsei, S., Hornyák, B., Kovácsházi, C., Brenner, G. B., Giricz, Z., Pálóczi, K., Kittel, Á., Tóvári, J., Turiak, L., Khamari, D., Ferdinandy, P., & Buzás, E. I. (2022). Circulating cardiomyocyte-derived extracellular vesicles reflect cardiac injury during systemic inflammatory response syndrome in mice. Cellular and Molecular Life Sciences, 79, 84.
Hell, S. W., & Wichmann, J. (1994). Breaking the diffraction resolution limit by stimulated emission: Stimulated-emission-depletion fluorescence microscopy. Optics Letters, 19, 780–782.
Hendrix, A., Lippens, L., Pinheiro, C., Théry, C., Martin-Jaular, L., Lötvall, J., Lässer, C., Hill, A F., & Witwer, K. W. (2023). Extracellular vesicle analysis. Nature Reviews Methods Primers, 3, 56.
Herrmann, I. K., Wood, M. J. A., & Fuhrmann, G. (2021). Extracellular vesicles as a next-generation drug delivery platform. Nature Nanotechnology, 16, 748–759.
Hess, S. T., Girirajan, T. P. K., & Mason, M. D. (2006). Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophysical Journal, 91, 4258–4272.
Heusermann, W., Hean, J., Trojer, D., Steib, E., von Bueren, S., Graff-Meyer, A., Genoud, C., Martin, K., Pizzato, N., Voshol, J., Morrissey, D. V., Andaloussi, S. E., Wood, M. J., & Meisner-Kober, N. C. (2016). Exosomes surf on filopodia to enter cells at endocytic hot spots, traffic within endosomes, and are targeted to the ER. Journal of Cell Biology, 213, 173–184.
Higginbotham, J. N., Demory Beckler, M., Gephart, J. D., Franklin, J. L., Bogatcheva, G., Kremers, G. J., Piston, D. W., Ayers, G. D., McConnell, R. E., Tyska, M. J., & Coffey, R. J. (2011). Amphiregulin exosomes increase cancer cell invasion. Current Biology, 21, 779–786.
Hill, A. F., Pegtel, D. M., Lambertz, U., Leonardi, T., O'Driscoll, L., Pluchino, S., Ter-Ovanesyan, D., & Nolte-’t Hoen, E. N. (2013). ISEV position paper: Extracellular vesicle RNA analysis and bioinformatics. Journal of Extracellular Vesicles, 2, 00–00.
Hogg, W. R., & Coulter, W. H. (1967). Apparatus and method for measuring a dividing particle size of a particulate system. In United States.
Hole, P., Sillence, K., Hannell, C., Maguire, C. M., Roesslein, M., Suarez, G., Capracotta, S., Magdolenova, Z., Horev-Azaria, L., Dybowska, A., Cooke, L., Haase, A., Contal, S., Mano, S., Vennemann, A., Sauvain, J. J., Staunton, K. C., Anguissola, S., Luch, A., … Wick, P. (2013). Interlaboratory comparison of size measurements on nanoparticles using nanoparticle tracking analysis (NTA). Journal of Nanoparticle Research, 15, 2101.
Hong, J., Dauros-Singorenko, P., Whitcombe, A., Payne, L., Blenkiron, C., Phillips, A., & Swift, S. (2019). Analysis of the Escherichia coli extracellular vesicle proteome identifies markers of purity and culture conditions. Journal of Extracellular Vesicles, 8, 1632099.
Hood, R. R., DeVoe, D. L., Atencia, J., Vreeland, W. N., & Omiatek, D. M. (2014). A facile route to the synthesis of monodisperse nanoscale liposomes using 3D microfluidic hydrodynamic focusing in a concentric capillary array. Lab on A Chip, 14, 2403–2409.
Hoog, J. L., & Lotvall, J. (2015). Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. Journal of Extracellular Vesicles, 4, 28680.
Hoshino, A., Costa-Silva, B., Shen, T. L., Rodrigues, G., Hashimoto, A., Tesic Mark, M., Molina, H., Kohsaka, S., Di Giannatale, A., Ceder, S., Singh, S., Williams, C., Soplop, N., Uryu, K., Pharmer, L., King, T., Bojmar, L., Davies, A. E., Ararso, Y., … Lyden, D. (2015). Tumour exosome integrins determine organotropic metastasis. Nature, 527, 329–335.
Hoshino, A., Kim, H. S., Bojmar, L., Gyan, K. E., Cioffi, M., Hernandez, J., Zambirinis, C. P., Rodrigues, G., Molina, H., Heissel, S., Mark, M. T., Steiner, L., Benito-Martin, A., Lucotti, S., Di Giannatale, A., Offer, K., Nakajima, M., Williams, C., Nogués, L., … Lyden, D. (2020). Extracellular vesicle and particle biomarkers define multiple human cancers. Cell, 182, 1044–1061.e18.
Howell, J. C., Watts, K. D., Parker, M. W., Wu, J., Kollhoff, A., Wingo, T. S., Dorbin, C. D., Qiu, D., & Hu, W. T. (2017). Race modifies the relationship between cognition and Alzheimer's disease cerebrospinal fluid biomarkers. Alzheimer's Research & Therapy, 9, 88.
Huang, Y., Cheng, L., Turchinovich, A., Mahairaki, V., Troncoso, J. C., Pletniková, O., Haughey, N. J., Vella, L. J., Hill, A. F., Zheng, L., & Witwer, K. W. (2020). Influence of species and processing parameters on recovery and content of brain tissue-derived extracellular vesicles. Journal of Extracellular Vesicles, 9, 1785746.
Hühmer, A. F., Biringer, R. G., Amato, H., Fonteh, A. N., & Harrington, M. G. (2006). Protein analysis in human cerebrospinal fluid: Physiological aspects, current progress and future challenges. Disease Markers, 22, 3–26.
Hurwitz, S. N., Olcese, J. M., & Meckes, D. G. Jr (2019). Extraction of extracellular vesicles from whole tissue. Journal of Visualized Experiments: JoVE, (144), https://doi.org/10.3791/59143
Hurwitz, S. N., Sun, L., Cole, K. Y., Ford, C. R. 3rd, Olcese, J. M., & Meckes, D. G. Jr (2018). An optimized method for enrichment of whole brain-derived extracellular vesicles reveals insight into neurodegenerative processes in a mouse model of Alzheimer's disease. Journal of Neuroscience Methods, 307, 210–220.
Hyenne, V., Apaydin, A., Rodriguez, D., Spiegelhalter, C., Hoff-Yoessle, S., Diem, M., Tak, S., Lefebvre, O., Schwab, Y., Goetz, J. G., & Labouesse, M. (2015). RAL-1 controls multivesicular body biogenesis and exosome secretion. Journal of Cell Biology, 211, 27–37.
Hyenne, V., Ghoroghi, S., Collot, M., Bons, J., Follain, G., Harlepp, S., Mary, B., Bauer, J., Mercier, L., Busnelli, I., Lefebvre, O., Fekonja, N., Garcia-Leon, M. J., Machado, P., Delalande, F., López, A. A., Silva, S. G., Verweij, F. J., van Niel, G., … Goetz, J. G. (2019). Studying the fate of tumor extracellular vesicles at high spatiotemporal resolution using the zebrafish embryo. Developmental Cell, 48, 554–572.e7.
Ishida, T., Hashimoto, T., Masaki, K., Funabashi, H., Hirota, R., Ikeda, T., Tajima, H., & Kuroda, A. (2020). Application of peptides with an affinity for phospholipid membranes during the automated purification of extracellular vesicles. Scientific Reports, 10, 18718.
Izumi, T. (2021). In vivo roles of Rab27 and its effectors in exocytosis. Cell Structure and Function, 46, 79–94.
Jack, C. R. Jr., Bennett, D. A., Blennow, K., Carrillo, M. C., Dunn, B., Haeberlein, S. B., Holtzman, D. M., Jagust, W., Jessen, F., Karlawish, J., Liu, E., Molinuevo, J. L., Montine, T., Phelps, C., Rankin, K. P., Rowe, C. C., Scheltens, P., Siemers, E., Snyder, H. M., & Sperling, R. (2018). NIA-AA research framework: Toward a biological definition of Alzheimer's disease. Alzheimers Dement, 14, 535–562.
Jang, S. C., Crescitelli, R., Cvjetkovic, A., Belgrano, V., Olofsson Bagge, R., Sundfeldt, K., Ochiya, T., Kalluri, R., & Lötvall, J. (2019). Mitochondrial protein enriched extracellular vesicles discovered in human melanoma tissues can be detected in patient plasma. Journal of Extracellular Vesicles, 8, 1635420.
Jaqaman, K., Loerke, D., Mettlen, M., Kuwata, H., Grinstein, S., Schmid, S. L., & Danuser, G. (2008). Robust single-particle tracking in live-cell time-lapse sequences. Nature Methods, 5, 695–702.
Jeppesen, D. K., Fenix, A. M., Franklin, J. L., Higginbotham, J. N., Zhang, Q., Zimmerman, L. J., Liebler, D. C., Ping, J., Liu, Q., Evans, R., Fissell, W. H., Patton, J. G., Rome, L. H., Burnette, D. T., & Coffey, R. J. (2019). Reassessment of exosome composition. Cell, 177, 428–445.e18.
Jeurissen, S., Vergauwen, G., Van Deun, J., Lapeire, L., Depoorter, V., Miinalainen, I., Sormunen, R., Van den Broecke, R., Braems, G., Cocquyt, V., Denys, H., & Hendrix, A. (2017). The isolation of morphologically intact and biologically active extracellular vesicles from the secretome of cancer-associated adipose tissue. Cell Adhesion & Migration, 11, 196–204.
Jewett, K. A., Thomas, R. E., Phan, C. Q., Lin, B., Milstein, G., Yu, S., Bettcher, L. F., Neto, F. C., Djukovic, D., Raftery, D., Pallanck, L. J., & Davis, M. Y. (2021). Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles. Plos Genetics, 17, e1008859.
Jingami, N., Uemura, K., Asada-Utsugi, M., Kuzuya, A., Yamada, S., Ishikawa, M., Kawahara, T., Iwasaki, T., Atsuchi, M., Takahashi, R., & Kinoshita, A. (2019). Two-point dynamic observation of Alzheimer's disease cerebrospinal fluid biomarkers in idiopathic normal pressure hydrocephalus. Journal of Alzheimer's Disease, 72, 271–277.
Jingushi, K., Uemura, M., Ohnishi, N., Nakata, W., Fujita, K., Naito, T., Fujii, R., Saichi, N., Nonomura, N., Tsujikawa, K., & Ueda, K. (2018). Extracellular vesicles isolated from human renal cell carcinoma tissues disrupt vascular endothelial cell morphology via azurocidin. International Journal of Cancer, 142, 607–617.
Johnsen, K. B., Gudbergsson, J. M., Andresen, T. L., & Simonsen, J. B. (2019). What is the blood concentration of extracellular vesicles? Implications for the use of extracellular vesicles as blood-borne biomarkers of cancer. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1871, 109–116.
Jones, R. R., Hooper, D. C., Zhang, L., Wolverson, D., & Valev, V. K. (2019). Raman techniques: Fundamentals and frontiers. Nanoscale Research Letters, 14, 231.
Joshi, B. S., de Beer, M. A., Giepmans, B. N. G., & Zuhorn, I. S. (2020). Endocytosis of extracellular vesicles and release of their cargo from endosomes. ACS Nano, 14, 4444–4455.
Jurgielewicz, B. J., Yao, Y., & Stice, S. L. (2020). Kinetics and specificity of HEK293T extracellular vesicle uptake using imaging flow cytometry. Nanoscale Research Letters, 15, 170.
Kaczor-Urbanowicz, K. E., Wei, F., Rao, S. L., Kim, J., Shin, H., Cheng, J., Tu, M., Wong, D. T. W., & Kim, Y. (2019). Clinical validity of saliva and novel technology for cancer detection. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1872, 49–59.
Kahlert, C., Melo, S. A., Protopopov, A., Tang, J., Seth, S., Koch, M., Zhang, J., Weitz, J., Chin, L., Futreal, A., & Kalluri, R. (2014). Identification of double-stranded genomic DNA spanning all chromosomes with mutated KRAS and p53 DNA in the serum exosomes of patients with pancreatic cancer. Journal of Biological Chemistry, 289, 3869–3875.
Kameli, N., Becker, H. E. F., Welbers, T., Jonkers, D., Penders, J., Savelkoul, P., & Stassen, F. R. (2021). Metagenomic profiling of fecal-derived bacterial membrane vesicles in Crohn's disease patients. Cells, 10(10), 2795.
Kang, M., Jordan, V., Blenkiron, C., & Chamley, L. W. (2021). Biodistribution of extracellular vesicles following administration into animals: A systematic review. Journal of Extracellular Vesicles, 10, e12085.
Karimi, N., Cvjetkovic, A., Jang, S. C., Crescitelli, R., Hosseinpour Feizi, M. A., Nieuwland, R., Lötvall, J., & Lässer, C. (2018). Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins. Cellular and Molecular Life Sciences, 75, 2873–2886.
Karimi, N., Dalirfardouei, R., Dias, T., Lötvall, J., & Lässer, C. (2022). Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma - Contributions of platelet extracellular vesicles in plasma samples. Journal of Extracellular Vesicles, 11, e12213.
Karttunen, J., Heiskanen, M., Navarro-Ferrandis, V., Gupta, S. D, Lipponen, A., Puhakka, N., Rilla, K., Koistinen, A., & Pitkänen, A. (2019). Precipitation-based extracellular vesicle isolation from rat plasma co-precipitate vesicle-free microRNAs. Journal of Extracellular Vesicles, 8, 1555410.
Keenan, J. I., & Allardyce, R. A. (2000). Iron influences the expression of Helicobacter pylori outer membrane vesicle-associated virulence factors. European Journal of Gastroenterology & Hepatology, 12, 1267–1273.
Keremi, B., Beck, A., Fabian, T. K., Fabian, G., Szabo, G., Nagy, A., & Varga, G. (2017). Stress and salivary glands. Current Pharmaceutical Design, 23, 4057–4065.
Kestens, V., Bozatzidis, V., De Temmerman, P. J., Ramaye, Y., & Roebben, G. (2017). Validation of a particle tracking analysis method for the size determination of nano- and microparticles. Journal of Nanoparticle Research, 19, 271.
Khater, I. M., Nabi, I. R., & Hamarneh, G. (2020). A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods. Patterns (N Y), 1, 100038.
Khurshid, Z., Zohaib, S., Najeeb, S., Zafar, M. S., Slowey, P. D., & Almas, K. (2016). Human saliva collection devices for proteomics: An update. International Journal of Molecular Sciences, 17, 846.
Klar, T. A., & Hell, S. W. (1999). Subdiffraction resolution in far-field fluorescence microscopy. Optics Letters, 24, 954–956.
Klener, J., Hofbauerová, K., Bartoš, A., Ríčný, J., Rípová, D., & Kopecký, V. (2014). Instability of cerebrospinal fluid after delayed storage and repeated freezing: A holistic study by drop coating deposition Raman spectroscopy. Clinical Chemistry and Laboratory Medicine, 52, 657–664.
Klont, F., Bras, L., Wolters, J. C., Ongay, S., Bischoff, R., Halmos, G. B., & Horvatovich, P. (2018). Assessment of sample preparation bias in mass spectrometry-based proteomics. Analytical Chemistry, 90, 5405–5413.
Kobayashi-Sun, J., Yamamori, S., Kondo, M., Kuroda, J., Ikegame, M., Suzuki, N., Kitamura, K. I., Hattori, A., Yamaguchi, M., & Kobayashi, I. (2020). Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale. Communications Biology, 3, 190.
Koles, K., Nunnari, J., Korkut, C., Barria, R., Brewer, C., Li, Y., Leszyk, J., Zhang, B., & Budnik, V. (2012). Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons. Journal of Biological Chemistry, 287, 16820–16834.
Kolhe, R., Owens, V., Sharma, A., Lee, T. J., Zhi, W., Ghilzai, U., Mondal, A. K., Liu, Y., Isales, C. M., Hamrick, M. W., Hunter, M., & Fulzele, S. (2020). Sex-specific differences in extracellular vesicle protein cargo in synovial fluid of patients with osteoarthritis. Life (Basel), 10(12), 337.
Koliha, N., Wiencek, Y., Heider, U., Jüngst, C., Kladt, N., Krauthäuser, S., Johnston, I. C., Bosio, A., Schauss, A., & Wild, S. (2016). A novel multiplex bead-based platform highlights the diversity of extracellular vesicles. Journal of Extracellular Vesicles, 5, 29975.
Korkut, C., Li, Y., Koles, K., Brewer, C., Ashley, J., Yoshihara, M., & Budnik, V. (2013). Regulation of postsynaptic retrograde signaling by presynaptic exosome release. Neuron, 77, 1039–1046.
Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016). Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. PNAS, 113, E968–E977.
Kreimer, S., Belov, A. M., Ghiran, I., Murthy, S. K., Frank, D. A., & Ivanov, A. R. (2015). Mass-spectrometry-based molecular characterization of extracellular vesicles: lipidomics and proteomics. Journal of Proteome Research, 14, 2367–2384.
Krušić Alić, V., Malenica, M., Biberić, M., Zrna, S., Valenčić, L., Šuput, A., Kalagac Fabris, L., Wechtersbach, K., Kojc, N., Kurtjak, M., Kučić, N., & Grabušić, K. (2022). Extracellular vesicles from human cerebrospinal fluid are effectively separated by sepharose CL-6B-comparison of four gravity-flow size exclusion chromatography methods. Biomedicines, 10, 785.
Kuehn, M. J., & Kesty, N. C. (2005). Bacterial outer membrane vesicles and the host-pathogen interaction. Genes & Development, 19, 2645–2655.
Kumeda, N., Ogawa, Y., Akimoto, Y., Kawakami, H., Tsujimoto, M., & Yanoshita, R. (2017). Characterization of membrane integrity and morphological stability of human salivary exosomes. Biological & Pharmaceutical Bulletin, 40, 1183–1191.
Kwizera, E. A., O'Connor, R., Vinduska, V., Williams, M., Butch, E. R., Snyder, S. E., Chen, X., & Huang, X. (2018). Molecular detection and analysis of exosomes using surface-enhanced Raman scattering gold nanorods and a miniaturized device. Theranostics, 8, 2722–2738.
Lacroix, R., Judicone, C., Poncelet, P., Robert, S., Arnaud, L., Sampol, J., & Dignat-George, F. (2012). Impact of pre-analytical parameters on the measurement of circulating microparticles: Towards standardization of protocol. Journal of Thrombosis and Haemostasis, 10, 437–446.
Lai, C. P., Kim, E. Y., Badr, C. E., Weissleder, R., Mempel, T. R., Tannous, B. A., & Breakefield, X. O. (2015). Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters. Nature Communications, 6, 7029.
Lamparski, H. G., Metha-Damani, A., Yao, J. Y., Patel, S., Hsu, D. H., Ruegg, C., & Le Pecq, J. B. (2002). Production and characterization of clinical grade exosomes derived from dendritic cells. Journal of Immunological Methods, 270, 211–226.
Langer, J., Jimenez de Aberasturi, D., Aizpurua, J., Alvarez-Puebla, R. A., Auguie, B., Baumberg, J. J., Bazan, G. C., Bell, S. E. J., Boisen, A., Brolo, A. G., Choo, J., Cialla-May, D., Deckert, V., Fabris, L., Faulds, K., Garcia de Abajo, F. J., Goodacre, R., Graham, D., Haes, A. J., … Liz-Marzan, L. M. (2020). Present and future of surface-enhanced Raman scattering. ACS Nano, 14, 28–117.
Lázaro-Ibáñez, E., Faruqu, F. N., Saleh, A. F., Silva, A. M., Tzu-Wen Wang, J., Rak, J., Al-Jamal, K. T., & Dekker, N. (2021). Selection of fluorescent, bioluminescent, and radioactive tracers to accurately reflect extracellular vesicle biodistribution in vivo. ACS Nano, 15, 3212–3227.
Lázaro-Ibáñez, E., Lässer, C., Shelke, G. V., Crescitelli, R., Jang, S. C., Cvjetkovic, A., García-Rodríguez, A., & Lötvall, J. (2019). DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology. Journal of Extracellular Vesicles, 8, 1656993.
Le Saux, S., Aarrass, H., Lai-Kee-Him, J., Bron, P., Armengaud, J., Miotello, G., Bertrand-Michel, J., Dubois, E., George, S., Faklaris, O., Devoisselle, J. M., Legrand, P., Chopineau, J., & Morille, M. (2020). Post-production modifications of murine mesenchymal stem cell (mMSC) derived extracellular vesicles (EVs) and impact on their cellular interaction. Biomaterials, 231, 119675.
LeClaire, M., Wohlschlegel, J. A., Chang, H. C., Wadehra, M., Yu, W., Rao, J., Elashoff, D., Gimzewski, J. K., & Sharma, S. (2021). Nanoscale extracellular vesicles carry the mechanobiology signatures of breast cancer cells. ACS Applied Nano Materials, 4, 9876–9885.
Lee, T. H., Chennakrishnaiah, S., Audemard, E., Montermini, L., Meehan, B., & Rak, J. (2014). Oncogenic ras-driven cancer cell vesiculation leads to emission of double-stranded DNA capable of interacting with target cells. Biochemical and Biophysical Research Communications, 451, 295–301.
Lee, W., Nanou, A., Rikkert, L., Coumans, F. A. W., Otto, C., Terstappen, L., & Offerhaus, H. L. (2018). Label-free prostate cancer detection by characterization of extracellular vesicles using raman spectroscopy. Analytical Chemistry, 90, 11290–11296.
Lehrich, B. M., Liang, Y., & Fiandaca, M. S. (2021). Foetal bovine serum influence on in vitro extracellular vesicle analyses. Journal of Extracellular Vesicles, 10, e12061.
Lehrich, B. M., Liang, Y., Khosravi, P., Federoff, H. J., & Fiandaca, M. S. (2018). Fetal bovine serum-derived extracellular vesicles persist within vesicle-depleted culture media. International Journal of Molecular Sciences, 19, 3538.
Lener, T., Gimona, M., Aigner, L., Börger, V., Buzas, E., Camussi, G., Chaput, N., Chatterjee, D., Court, F. A., del Portillo, H. A., O'Driscoll, L., Fais, S., Falcon-Perez, J. M., Felderhoff-Mueser, U., Fraile, L., Gho, Y. S., Görgens, A., Gupta, R. C., Hendrix, A., … Giebel, B. (2015). Applying extracellular vesicles based therapeutics in clinical trials – An ISEV position paper. Journal of Extracellular Vesicles, 4, 30087.
Lennon, K. M., Wakefield, D. L., Maddox, A. L., Brehove, M. S., Willner, A. N., Garcia-Mansfield, K., Meechoovet, B., Reiman, R., Hutchins, E., Miller, M. M., Goel, A., Pirrotte, P., Van Keuren-Jensen, K., & Jovanovic-Talisman, T. (2019). Single molecule characterization of individual extracellular vesicles from pancreatic cancer. Journal of Extracellular Vesicles, 8, 1685634.
Lewczuk, P., Beck, G., Ganslandt, O., Esselmann, H., Deisenhammer, F., Regeniter, A., Petereit, H. F., Tumani, H., Gerritzen, A., Oschmann, P., Schröder, J., Schönknecht, P., Zimmermann, K., Hampel, H., Bürger, K., Otto, M., Haustein, S., Herzog, K., Dannenberg, R., … Wiltfang, J. (2006). International quality control survey of neurochemical dementia diagnostics. Neuroscience Letters, 409, 1–4.
Li, D., Shao, L., Chen, B. C., Zhang, X., Zhang, M., Moses, B., Milkie, D. E., Beach, J. R., Hammer, J. A. 3rd, Pasham, M., Kirchhausen, T., Baird, M. A., Davidson, M. W., Xu, P., & Betzig, E. (2015). ADVANCED IMAGING. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science, 349, aab3500.
Li, G., Shofer, J. B., Petrie, E. C., Yu, C. E., Wilkinson, C. W., Figlewicz, D. P., Shutes-David, A., Zhang, J., Montine, T. J., Raskind, M. A., Quinn, J. F., Galasko, D. R., & Peskind, E. R. (2017). Cerebrospinal fluid biomarkers for Alzheimer's and vascular disease vary by age, gender, and APOE genotype in cognitively normal adults. Alzheimer's Research & Therapy, 9, 48.
Li-Hui, W., Chuan-Quan, L., Long, Y., Ru-Liu, L., Long-Hui, C., & Wei-Wen, C. (2016). Gender differences in the saliva of young healthy subjects before and after citric acid stimulation. Clinica Chimica Acta, 460, 142–145.
Liangsupree, T., Multia, E., & Riekkola, M. L. (2021). Modern isolation and separation techniques for extracellular vesicles. Journal of Chromatography A, 1636, 461773.
Liao, Z., Jaular, L. M., Soueidi, E., Jouve, M., Muth, D. C., Schøyen, T. H., Seale, T., Haughey, N. J., Ostrowski, M., Théry, C., & Witwer, K. W. (2019). Acetylcholinesterase is not a generic marker of extracellular vesicles. Journal of Extracellular Vesicles, 8, 1628592.
Liebler, D. C., & Zimmerman, L. J. (2013). Targeted quantitation of proteins by mass spectrometry. Biochemistry, 52, 3797–3806.
Liégeois, S., Benedetto, A., Garnier, J. M., Schwab, Y., & Labouesse, M. (2006). The V0-ATPase mediates apical secretion of exosomes containing Hedgehog-related proteins in Caenorhabditis elegans. Journal of Cell Biology, 173, 949–961.
Ligtenberg, A. J., Liem, E. H., Brand, H. S., & Veerman, E. C. (2016). The effect of exercise on salivary viscosity. Diagnostics (Basel), 6, 40.
Lim, G. T., You, D. G., Han, H. S., Lee, H., Shin, S., Oh, B. H., Kumar, E. K. P., Um, W., Kim, C. H., Han, S., Lee, S., Lim, S., Yoon, H. Y., Kim, K., Kwon, I. C., Jo, D. G., Cho, Y. W., & Park, J. H. (2021). Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases. Journal of Extracellular Vesicles, 10, e12077.
Limbutara, K., Chou, C. L., & Knepper, M. A. (2020). Quantitative proteomics of all 14 renal tubule segments in rat. Journal of the American Society of Nephrology, 31, 1255–1266.
Linares, R., Tan, S., Gounou, C., Arraud, N., & Brisson, A. R. (2015). High-speed centrifugation induces aggregation of extracellular vesicles. Journal of Extracellular Vesicles, 4, 29509.
Lischnig, A., Bergqvist, M., Ochiya, T., & Lässer, C. (2022). Quantitative proteomics identifies proteins enriched in large and small extracellular vesicles. Molecular & Cellular Proteomics, 21, 100273.
Liu, D., Kou, X., Chen, C., Liu, S., Liu, Y., Yu, W., Yu, T., Yang, R., Wang, R., Zhou, Y., & Shi, S. (2018). Circulating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors. Cell Research, 28, 918–933.
Liu, H., Tian, Y., Xue, C., Niu, Q., Chen, C., & Yan, X. (2022). Analysis of extracellular vesicle DNA at the single-vesicle level by nano-flow cytometry. Journal of Extracellular Vesicles, 11, e12206.
Liu, Q., Rojas-Canales, D. M., Divito, S. J., Shufesky, W. J., Stolz, D. B., Erdos, G., Sullivan, M. L., Gibson, G. A., Watkins, S. C., Larregina, A. T., & Morelli, A. E. (2016). Donor dendritic cell-derived exosomes promote allograft-targeting immune response. Journal of Clinical Investigation, 126, 2805–2820.
Liu, X., Yang, X., Sun, W., Wu, Q., Song, Y., Yuan, L., & Yang, G. (2019). Systematic evolution of ligands by exosome enrichment: A proof-of-concept study for exosome-based targeting peptide screening. Advanced Biosystems, 3, e1800275.
Liu, Y., Li, H., Wang, J., Xue, Q., Yang, X., Kang, Y., Li, M., Xu, J., Li, G., Li, C., Chang, H. C., Su, K. P., & Wang, F. (2020). Association of cigarette smoking with cerebrospinal fluid biomarkers of neurodegeneration, neuroinflammation, and oxidation. JAMA Network Open, 3, e2018777.
Liu, Z., Cauvi, D. M., Bernardino, E. M. A., Lara, B., Lizardo, R. E., Hawisher, D., Bickler, S., & De Maio, A. (2018). Isolation and characterization of human urine extracellular vesicles. Cell Stress & Chaperones, 23, 943–953.
Lobb, R. J., Becker, M., Wen, S. W., Wong, C. S., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015). Optimized exosome isolation protocol for cell culture supernatant and human plasma. Journal of Extracellular Vesicles, 4, 27031.
Long, Q., Upadhya, D., Hattiangady, B., Kim, D. K., An, S. Y., Shuai, B., Prockop, D. J., & Shetty, A. K. (2017). Intranasal MSC-derived A1-exosomes ease inflammation, and prevent abnormal neurogenesis and memory dysfunction after status epilepticus. PNAS, 114, E3536–E3545.
López-Guerrero, J. A., Valés-Gómez, M., Borrás, F. E., Falcón-Pérez, J. M., Vicent, M. J., & Yáñez-Mó, M. (2023). Standardising the preanalytical reporting of biospecimens to improve reproducibility in extracellular vesicle research – A GEIVEX study. Journal of Extracellular Biology, 2, e76.
Lőrincz, Á. M., Timár, C. I., Marosvári, K. A., Veres, D. S., Otrokocsi, L., Kittel, Á., & Ligeti, E. (2014). Effect of storage on physical and functional properties of extracellular vesicles derived from neutrophilic granulocytes. Journal of Extracellular Vesicles, 3, 25465.
Lotvall, J., Hill, A. F., Hochberg, F., Buzas, E. I., Di Vizio, D., Gardiner, C., Gho, Y. S., Kurochkin, I. V., Mathivanan, S., Quesenberry, P., Sahoo, S., Tahara, H., Wauben, M. H., Witwer, K. W., & Thery, C. (2014). Minimal experimental requirements for definition of extracellular vesicles and their functions: A position statement from the International Society for Extracellular Vesicles. Journal of Extracellular Vesicles, 3, 26913.
Lucas, M., Ryan, J. M., Watkins, J., Early, K., Kruh-Garcia, N. A., Mehaffy, C., & Dobos, K. M. (2021). Extraction and separation of mycobacterial proteins. Methods in Molecular Biology, 2314, 77–107.
Lucey, B. P., Fagan, A. M., Holtzman, D. M., Morris, J. C., & Bateman, R. J. (2017). Diurnal oscillation of CSF Aβ and other AD biomarkers. Molecular Neurodegeneration, 12, 36.
Lucien, F., Gustafson, D., Lenassi, M., Li, B., Teske, J. J., Boilard, E., Clemm von Hohenberg, K., Falcón-Perez, J. M., Gualerzi, A., Reale, A., Jones, J. C., Lässer, C., Lawson, C., Nazarenko, I., O'Driscoll, L., Pink, R., Siljander, P. R.-M., Soekmadji, C., Hendrix, A., … Nieuwland, R. (2023). MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research. Journal of Extracellular Vesicles, 12(12), e12385.
Lunavat, T. R., Cheng, L., Einarsdottir, B. O., Olofsson Bagge, R., Veppil Muralidharan, S., Sharples, R. A., Lässer, C., Gho, Y. S., Hill, A. F., Nilsson, J. A., & Lötvall, J. (2017). BRAF(V600) inhibition alters the microRNA cargo in the vesicular secretome of malignant melanoma cells. PNAS, 114, E5930–E5939.
Lundy, S. K., Klinker, M. W., & Fox, D. A. (2015). Killer B lymphocytes and their fas ligand positive exosomes as inducers of immune tolerance. Frontiers in Immunology, 6, 122.
Luo, W., Dai, Y., Chen, Z., Yue, X., Andrade-Powell, K. C., & Chang, J. (2020). Spatial and temporal tracking of cardiac exosomes in mouse using a nano-luciferase-CD63 fusion protein. Communications Biology, 3, 114.
Ma, D., Huang, C., Zheng, J., Tang, J., Li, J., Yang, J., & Yang, R. (2018). Quantitative detection of exosomal microRNA extracted from human blood based on surface-enhanced Raman scattering. Biosensors & Bioelectronics, 101, 167–173.
Mackenzie, K., Foot, N. J., Anand, S., Dalton, H. E., Chaudhary, N., Collins, B. M., Mathivanan, S., & Kumar, S. (2016). Regulation of the divalent metal ion transporter via membrane budding. Cell Discovery, 2, 16011.
Maire, C. L., Fuh, M. M., Kaulich, K., Fita, K. D., Stevic, I., Heiland, D. H., Welsh, J. A., Jones, J. C., Görgens, A., Ricklefs, T., Dührsen, L., Sauvigny, T., Joosse, S. A., Reifenberger, G., Pantel, K., Glatzel, M., Miklosi, A. G., Felce, J. H., Caselli, M., … Ricklefs, F. L. (2021). Genome-wide methylation profiling of glioblastoma cell-derived extracellular vesicle DNA allows tumor classification. Neuro-oncol, 23, 1087–1099.
Marie, P. P., Fan, S. J., Mason, J., Wells, A., Mendes, C. C., Wainwright, S. M., Scott, S., Fischer, R., Harris, A. L., Wilson, C., & Goberdhan, D. C. I. (2023). Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation. Journal of Extracellular Vesicles, 12, e12311.
Martin-Jaular, L., Nevo, N., Schessner, J. P., Tkach, M., Jouve, M., Dingli, F., Loew, D., Witwer, K. W., Ostrowski, M., Borner, G. H. H., & Théry, C. (2021). Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. Embo Journal, 40, e105492.
Martinez-Bartolome, S., Deutsch, E. W., Binz, P. A., Jones, A. R., Eisenacher, M., Mayer, G., Campos, A., Canals, F., Bech-Serra, J. J., Carrascal, M., Gay, M., Paradela, A., Navajas, R., Marcilla, M., Hernaez, M. L., Gutierrez-Blazquez, M. D., Velarde, L. F., Aloria, K., Beaskoetxea, J., … Albar, J. P. (2013). Guidelines for reporting quantitative mass spectrometry based experiments in proteomics. Journal of Proteomics, 95, 84–88.
Mateescu, B., Kowal, E. J., van Balkom, B. W., Bartel, S., Bhattacharyya, S. N., Buzas, E. I., Buck, A. H., de Candia, P., Chow, F. W., Das, S., Driedonks, T. A., Fernandez-Messina, L., Haderk, F., Hill, A. F., Jones, J. C., Van Keuren-Jensen, K. R., Lai, C. P., Lasser, C., Liegro, I. D., … Nolte-’t Hoen, E. N. (2017). Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – An ISEV position paper. Journal of Extracellular Vesicles, 6, 1286095.
Mathieu, M., Martin-Jaular, L., Lavieu, G., & Théry, C. (2019). Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication. Nature Cell Biology, 21, 9–17.
Mathieu, M., Névo, N., Jouve, M., Valenzuela, J. I., Maurin, M., Verweij, F. J., Palmulli, R., Lankar, D., Dingli, F., Loew, D., Rubinstein, E., Boncompain, G., Perez, F., & Théry, C. (2021). Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nature Communications, 12, 4389.
Mattsson, N., Andreasson, U., Persson, S., Arai, H., Batish, S. D., Bernardini, S., Bocchio-Chiavetto, L., Blankenstein, M. A., Carrillo, M. C., Chalbot, S., Coart, E., Chiasserini, D., Cutler, N., Dahlfors, G., Duller, S., Fagan, A. M., Forlenza, O., Frisoni, G. B., Galasko, D., … Blennow, K. (2011). The Alzheimer's Association external quality control program for cerebrospinal fluid biomarkers. Alzheimers Dement, 7, 386–395.e6.
Matusek, T., Wendler, F., Polès, S., Pizette, S., D'Angelo, G., Fürthauer, M., & Thérond, P. P. (2014). The ESCRT machinery regulates the secretion and long-range activity of Hedgehog. Nature, 516, 99–103.
McCann, J. V., Bischoff, S. R., Zhang, Y., Cowley, D. O., Sanchez-Gonzalez, V., Daaboul, G. D., & Dudley, A. C. (2020). Reporter mice for isolating and auditing cell type-specific extracellular vesicles in vivo. Genesis (New York, N.Y.: 2000), 58, e23369.
McKenzie, A. J., Hoshino, D., Hong, N. H., Cha, D. J., Franklin, J. L., Coffey, R. J., Patton, J. G., & Weaver, A. M. (2016). KRAS-MEK signaling controls Ago2 sorting into exosomes. Cell Reports, 15, 978–987.
McMillan, H. M., & Kuehn, M. J. (2023). Proteomic profiling reveals distinct bacterial extracellular vesicle subpopulations with possibly unique functionality. Applied and Environmental Microbiology, 89, e0168622.
Mehanny, M., Kroniger, T., Koch, M., Hoppstädter, J., Becher, D., Kiemer, A. K., Lehr, C. M., & Fuhrmann, G. (2022). Yields and immunomodulatory effects of pneumococcal membrane vesicles differ with the bacterial growth phase. Advanced Healthcare Materials, 11, e2101151.
Michael, B. N. R., Kommoju, V., Ganapathy, C. K, & Negi, V. S. (2019). Characterization of cell-derived microparticles in synovial fluid and plasma of patients with rheumatoid arthritis. Rheumatology International, 39, 1377–1387.
Mihaly, J., Deak, R., Szigyarto, I. C., Bota, A., Beke-Somfai, T., & Varga, Z. (2017). Characterization of extracellular vesicles by IR spectroscopy: Fast and simple classification based on amide and CH stretching vibrations. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1859, 459–466.
Mittelbrunn, M., Gutiérrez-Vázquez, C., Villarroya-Beltri, C., González, S., Sánchez-Cabo, F., González, M. Á., Bernad, A., & Sánchez-Madrid, F. (2011). Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. Nature Communications, 2, 282.
Möhrmann, L., Huang, H. J., Hong, D. S., Tsimberidou, A. M., Fu, S., Piha-Paul, S. A., Subbiah, V., Karp, D. D., Naing, A., Krug, A., Enderle, D., Priewasser, T., Noerholm, M., Eitan, E., Coticchia, C., Stoll, G., Jordan, L. M., Eng, C., Kopetz, E. S., … Janku, F. (2018). Liquid biopsies using plasma exosomal nucleic acids and plasma cell-free DNA compared with clinical outcomes of patients with advanced cancers. Clinical Cancer Research, 24, 181–188.
Mondal, A., Ashiq, K. A., Phulpagar, P., Singh, D. K., & Shiras, A. (2019). Effective visualization and easy tracking of extracellular vesicles in glioma cells. Biological Procedures Online, 21, 4.
Montero Llopis, P., Senft, R. A., Ross-Elliott, T. J., Stephansky, R., Keeley, D. P., Koshar, P., Marqués, G., Gao, Y. S., Carlson, B. R., Pengo, T., Sanders, M. A., Cameron, L. A., & Itano, M. S. (2021). Best practices and tools for reporting reproducible fluorescence microscopy methods. Nature Methods, 18(12), 1463–1476.
Morales-Kastresana, A., Musich, T. A., Welsh, J. A., Telford, W., Demberg, T., Wood, J. C. S., Bigos, M., Ross, C. D., Kachynski, A., Dean, A., Felton, E. J., van Dyke, J., Tigges, J., Toxavidis, V., Parks, D. R., Overton, W. R., Kesarwala, A. H., Freeman, G. J., Rosner, A., … Jones, J. C. (2019). High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer. Journal of Extracellular Vesicles, 8, 1597603.
Morikawa, Y., Takahashi, N., Kamiyama, K., Nishimori, K., Nishikawa, Y., Morita, S., Kobayashi, M., Fukushima, S., Yokoi, S., Mikami, D., Kimura, H., Kasuno, K., Yashiki, T., Naiki, H., Hara, M., & Iwano, M. (2019). Elevated levels of urinary extracellular vesicle fibroblast-specific protein 1 in patients with active crescentic glomerulonephritis. Nephron, 141, 177–187.
Mukhopadhya, A., Santoro, J., Moran, B., Useckaite, Z., & O'Driscoll, L. (2021). Optimisation and comparison of orthogonal methods for separation and characterisation of extracellular vesicles to investigate how representative infant milk formula is of milk. Food Chemistry, 353, 129309.
Mukhopadhya, A., Santoro, J., & O'Driscoll, L. (2021). Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation. STAR Protocols, 2, 100821.
Musicò, A., Zenatelli, R., Romano, M., Zendrini, A., Alacqua, S., Tassoni, S., Paolini, L., Urbinati, C., Rusnati, M., Bergese, P., Pomarico, G., & Radeghieri, A. (2023). Surface functionalization of extracellular vesicle nanoparticles with antibodies: A first study on the protein corona “variable”. Nanoscale Advances, 5, 4703–4717.
Mustonen, A. M., Capra, J., Rilla, K., Lehenkari, P., Oikari, S., Kääriäinen, T., Joukainen, A., Kröger, H., Paakkonen, T., Matilainen, J., & Nieminen, P. (2021). Characterization of hyaluronan-coated extracellular vesicles in synovial fluid of patients with osteoarthritis and rheumatoid arthritis. BMC Musculoskeletal Disorders [Electronic Resource], 22, 247.
Nakai, W., Yoshida, T., Diez, D., Miyatake, Y., Nishibu, T., Imawaka, N., Naruse, K., Sadamura, Y., & Hanayama, R. (2016). A novel affinity-based method for the isolation of highly purified extracellular vesicles. Scientific Reports, 6, 33935.
Nakayasu, E. S., Gritsenko, M., Piehowski, P. D., Gao, Y., Orton, D. J., Schepmoes, A. A., Fillmore, T. L., Frohnert, B. I., Rewers, M., Krischer, J. P., Ansong, C., Suchy-Dicey, A. M., Evans-Molina, C., Qian, W. J., Webb-Robertson, B. M., & Metz, T. O. (2021). Tutorial: Best practices and considerations for mass-spectrometry-based protein biomarker discovery and validation. Nature Protocols, 16, 3737–3760.
Navazesh, M. (1993). Methods for collecting saliva. Annals of the New York Academy of Sciences, 694, 72–77.
Neckles, V. N., Morton, M. C., Holmberg, J. C., Sokolov, A. M., Nottoli, T., Liu, D., & Feliciano, D. M. (2019). A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles. Scientific Reports, 9, 3094.
Newman, L A., Useckaite, Z., & Rowland, A. (2022). Addressing MISEV guidance using targeted LC-MS/MS: A method for the detection and quantification of extracellular vesicle-enriched and contaminant protein markers from blood. Journal of Extracellular Biology, 1, e56.
Ngamchuea, K., Chaisiwamongkhol, K., Batchelor-McAuley, C., & Compton, R. G. (2017). Chemical analysis in saliva and the search for salivary biomarkers – A tutorial review. Analyst, 143, 81–99.
Nguyen, V. V. T., Witwer, K. W., Verhaar, M. C., Strunk, D., & van Balkom, B. W. M. (2020). Functional assays to assess the therapeutic potential of extracellular vesicles. Journal of Extracellular Vesicles, 10, e12033.
Nieuwland, R., Falcon-Perez, J. M., Thery, C., & Witwer, K. W. (2020). Rigor and standardization of extracellular vesicle research: Paving the road towards robustness. Journal of Extracellular Vesicles, 10, e12037.
Nikonorova, I. A., Wang, J., Cope, A. L., Tilton, P. E., Power, K. M., Walsh, J. D., Akella, J. S., Krauchunas, A. R., Shah, P., & Barr, M. M. (2022). Isolation, profiling, and tracking of extracellular vesicle cargo in Caenorhabditis elegans. Current Biology, 32(9), 1924–1936.e6.
Nizamudeen, Z., Markus, R., Lodge, R., Parmenter, C., Platt, M., Chakrabarti, L., & Sottile, V. (2018). Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865, 1891–1900.
Nonaka, T., & Wong, D. T. W. (2022). Saliva diagnostics. Annual Review of Analytical Chemistry, 15, 107–121.
Nørgård, M. Ø., Steffensen, L. B., Hansen, D. R., Füchtbauer, E. M., Engelund, M. B., Dimke, H., Andersen, D. C., & Svenningsen, P. (2022). A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles. Scientific Reports, 12, 496.
Norman, M., Ter-Ovanesyan, D., Trieu, W., Lazarovits, R., Kowal, E. J. K., Lee, J. H., Chen-Plotkin, A. S., Regev, A., Church, G. M., & Walt, D. R. (2021). L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma. Nature Methods, 18, 631–634.
Obeid, S., Sung, P. S., Roy, B. L., Chou, M. L., Hsieh, S. L., Elie-Caille, C., Burnouf, T., & Boireau, W. (2019). NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety. Nanomedicine, 20, 101977.
Ogawa, Y., Kanai-Azuma, M., Akimoto, Y., Kawakami, H., & Yanoshita, R. (2008). Exosome-like vesicles with dipeptidyl peptidase IV in human saliva. Biological & Pharmaceutical Bulletin, 31, 1059–1062.
Oleksiuk, O., Abba, M., Tezcan, K. C., Schaufler, W., Bestvater, F., Patil, N., Birk, U., Hafner, M., Altevogt, P., Cremer, C., & Allgayer, H. (2015). Single-Molecule Localization Microscopy allows for the analysis of cancer metastasis-specific miRNA distribution on the nanoscale. Oncotarget, 6, 44745–44757.
Oliveira, D. L., Nakayasu, E. S., Joffe, L. S., Guimarães, A. J., Sobreira, T. J., Nosanchuk, J. D., Cordero, R. J., Frases, S., Casadevall, A., Almeida, I. C., Nimrichter, L., & Rodrigues, M. L. (2010). Characterization of yeast extracellular vesicles: evidence for the participation of different pathways of cellular traffic in vesicle biogenesis. PLoS ONE, 5, e11113.
Osteikoetxea, X., Sodar, B., Nemeth, A., Szabo-Taylor, K., Paloczi, K., Vukman, K. V., Tamasi, V., Balogh, A., Kittel, A., Pallinger, E., & Buzas, E. I. (2015). Differential detergent sensitivity of extracellular vesicle subpopulations. Organic & Biomolecular Chemistry, 13, 9775–9782.
Ostrowski, M., Carmo, N. B., Krumeich, S., Fanget, I., Raposo, G., Savina, A., Moita, C. F., Schauer, K., Hume, A. N., Freitas, R. P., Goud, B., Benaroch, P., Hacohen, N., Fukuda, M., Desnos, C., Seabra, M. C., Darchen, F., Amigorena, S., Moita, L. F., & Thery, C. (2010). Rab27a and Rab27b control different steps of the exosome secretion pathway. Nature Cell Biology, 12, 19–30, 1–13.
Page, M. J., Kell, D. B., & Pretorius, E. (2022). The role of lipopolysaccharide-induced cell signalling in chronic inflammation. Chronic Stress (Thousand Oaks), 6, 24705470221076390.
Palma, J., Yaddanapudi, S. C., Pigati, L., Havens, M. A., Jeong, S., Weiner, G. A., Weimer, K. M., Stern, B., Hastings, M. L., & Duelli, D. M. (2012). MicroRNAs are exported from malignant cells in customized particles. Nucleic Acids Research, 40, 9125–9138.
Palmieri, V., Lucchetti, D., Gatto, I., Maiorana, A., Marcantoni, M., Maulucci, G., Papi, M., Pola, R., De Spirito, M., & Sgambato, A. (2014). Dynamic light scattering for the characterization and counting of extracellular vesicles: A powerful noninvasive tool. Journal of Nanoparticle Research, 16, 2583.
Palviainen, M., Saari, H., Kärkkäinen, O., Pekkinen, J., Auriola, S., Yliperttula, M., Puhka, M., Hanhineva, K., & Siljander, P. R. (2019). Metabolic signature of extracellular vesicles depends on the cell culture conditions. Journal of Extracellular Vesicles, 8, 1596669.
Palviainen, M., Saraswat, M., Varga, Z., Kitka, D., Neuvonen, M., Puhka, M., Joenväärä, S., Renkonen, R., Nieuwland, R., Takatalo, M., & Siljander, P. R. M. (2020). Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo-Implications for biomarker discovery. PLoS ONE, 15, e0236439.
Panagopoulou, M. S., Wark, A. W., Birch, D. J. S., & Gregory, C. D. (2020). Phenotypic analysis of extracellular vesicles: A review on the applications of fluorescence. Journal of Extracellular Vesicles, 9, 1710020.
Paolini, L., Federici, S., Consoli, G., Arceri, D., Radeghieri, A., Alessandri, I., & Bergese, P. (2020). Fourier-transform Infrared (FT-IR) spectroscopy fingerprints subpopulations of extracellular vesicles of different sizes and cellular origin. Journal of Extracellular Vesicles, 9, 1741174.
Paolini, L., Zendrini, A., Di Noto, G., Busatto, S., Lottini, E., Radeghieri, A., Dossi, A., Caneschi, A., Ricotta, D., & Bergese, P. (2016). Residual matrix from different separation techniques impacts exosome biological activity. Scientific Reports, 6, 23550.
Parisse, P., Rago, I., Ulloa Severino, L., Perissinotto, F., Ambrosetti, E., Paoletti, P., Ricci, M., Beltrami, A. P., Cesselli, D., & Casalis, L. (2017). Atomic force microscopy analysis of extracellular vesicles. European Biophysics Journal, 46, 813–820.
Park, J., Hwang, M., Choi, B., Jeong, H., Jung, J. H., Kim, H. K., Hong, S., Park, J. H., & Choi, Y. (2017). Exosome classification by pattern analysis of surface-enhanced raman spectroscopy data for lung cancer diagnosis. Analytical Chemistry, 89, 6695–6701.
Peinado, H., Alečković, M., Lavotshkin, S., Matei, I., Costa-Silva, B., Moreno-Bueno, G., Hergueta-Redondo, M., Williams, C., García-Santos, G., Ghajar, C., Nitadori-Hoshino, A., Hoffman, C., Badal, K., Garcia, B. A., Callahan, M. K., Yuan, J., Martins, V. R., Skog, J., Kaplan, R. N., … Lyden, D. (2012). Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nature Medicine, 18, 883–891.
Perez-Gonzalez, R., Gauthier, S. A., Kumar, A., & Levy, E. (2012). The exosome secretory pathway transports amyloid precursor protein carboxyl-terminal fragments from the cell into the brain extracellular space. Journal of Biological Chemistry, 287, 43108–43115.
Perrin, P., Janssen, L., Janssen, H., van den Broek, B., Voortman, L. M., van Elsland, D., Berlin, I., & Neefjes, J. (2021). Retrofusion of intralumenal MVB membranes parallels viral infection and coexists with exosome release. Current Biology, 31, 3884–3893.e4.
Pfeiler, S., Thakur, M., Grünauer, P., Megens, R. T. A., Joshi, U., Coletti, R., Samara, V., Müller-Stoy, G., Ishikawa-Ankerhold, H., Stark, K., Klingl, A., Fröhlich, T., Arnold, G. J., Wörmann, S., Bruns, C. J., Algül, H., Weber, C., Massberg, S., & Engelmann, B. (2019). CD36-triggered cell invasion and persistent tissue colonization by tumor microvesicles during metastasis. Faseb Journal, 33, 1860–1872.
Pham, T. C., Jayasinghe, M. K., Pham, T. T., Yang, Y., Wei, L., Usman, W. M., Chen, H., Pirisinu, M., Gong, J., Kim, S., Peng, B., Wang, W., Chan, C., Ma, V., Nguyen, N. T. H., Kappei, D., Nguyen, X. H., Cho, W. C., Shi, J., & Le, M. T. N. (2021). Covalent conjugation of extracellular vesicles with peptides and nanobodies for targeted therapeutic delivery. Journal of Extracellular Vesicles, 10, e12057.
Piontek, M. C., Lira, R. B., & Roos, W. H. (2021). Active probing of the mechanical properties of biological and synthetic vesicles. Biochimica et Biophysica Acta (BBA) - General Subjects, 1865, 129486.
Pisitkun, T., Shen, R. F., & Knepper, M. A. (2004). Identification and proteomic profiling of exosomes in human urine. PNAS, 101, 13368–13373.
Pleet, M. L., Cook, S., Tang, V. A., Stack, E., Ford, V. J., Lannigan, J., Do, N., Wenger, E., Fraikin, J. L., Jacobson, S., Jones, J. C., & Welsh, J. A. (2023). Extracellular vesicle refractive index derivation utilizing orthogonal characterization. Nano Letters, 23(20), 9195–9202.
Pocsfalvi, G., Stanly, C., Vilasi, A., Fiume, I., Capasso, G., Turiak, L., Buzas, E. I., & Vekey, K. (2016). Mass spectrometry of extracellular vesicles. Mass Spectrometry Reviews, 35, 3–21.
Pocsfalvi, G., Stanly, C., Fiume, I., & Vékey, K. (2016). Chromatography and its hyphenation to mass spectrometry for extracellular vesicle analysis. Journal of Chromatography A, 1439, 26–41.
Polanco, J. C., Li, C., Durisic, N., Sullivan, R., & Götz, J. (2018). Exosomes taken up by neurons hijack the endosomal pathway to spread to interconnected neurons. Acta Neuropathologica Communications, 6, 10.
Polanco, J. C., Scicluna, B. J., Hill, A. F., & Götz, J. (2016). Extracellular vesicles isolated from the brains of rTg4510 mice seed tau protein aggregation in a threshold-dependent manner. Journal of Biological Chemistry, 291, 12445–12466.
Prados-Rosales, R., Baena, A., Martinez, L. R., Luque-Garcia, J., Kalscheuer, R., Veeraraghavan, U., Camara, C., Nosanchuk, J. D., Besra, G. S., Chen, B., Jimenez, J., Glatman-Freedman, A., Jacobs, W. R., Jr., Porcelli, S. A., & Casadevall, A. (2011). Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice. Journal of Clinical Investigation, 121, 1471–1483.
Preußer, C., Stelter, K., Tertel, T., Linder, M., Helmprobst, F., Szymanski, W., Graumann, J., Giebel, B., Reinartz, S., Müller, R., Weber, G., & von Strandmann, E. P. (2022). Isolation of native EVs from primary biofluids—Free-flow electrophoresis as a novel approach to purify ascites-derived EVs. Journal of Extracellular Biology, 1, e71.
Provencher, S. W. (1982). A constrained regularization method for inverting data represented by linear algebraic or integral equations. Computer Physics Communications, 27, 213–227.
Puca, L., Chastagner, P., Meas-Yedid, V., Israël, A., & Brou, C. (2013). Α-arrestin 1 (ARRDC1) and β-arrestins cooperate to mediate Notch degradation in mammals. Journal of Cell Science, 126, 4457–4468.
Pucci, F., Garris, C., Lai, C. P., Newton, A., Pfirschke, C., Engblom, C., Alvarez, D., Sprachman, M., Evavold, C., Magnuson, A., von Andrian, U. H., Glatz, K., Breakefield, X. O., Mempel, T. R., Weissleder, R., & Pittet, M. J. (2016). SCS macrophages suppress melanoma by restricting tumor-derived vesicle-B cell interactions. Science, 352, 242–246.
Pužar Dominkuš, P., Stenovec, M., Sitar, S., Lasič, E., Zorec, R., Plemenitaš, A., Žagar, E., Kreft, M., & Lenassi, M. (2018). PKH26 labeling of extracellular vesicles: Characterization and cellular internalization of contaminating PKH26 nanoparticles. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860, 1350–1361.
Qu, X., Li, Q., Yang, J., Zhao, H., Wang, F., Zhang, F., Zhang, S., Zhang, H., Wang, R., Wang, Q., Wang, Q., Li, G., Peng, X., Zhou, X., Hao, Y., Zhu, J., & Xiao, W. (2019). Double-stranded DNA in exosomes of malignant pleural effusions as a novel DNA source for EGFR mutation detection in lung adenocarcinoma. Frontiers in Oncology, 9, 931.
Rad, M., Kakoie, S., Brojeni, F. N, & Pourdamghan, N. (2010). Effect of long-term smoking on whole-mouth salivary flow rate and oral Health. Journal of Dental Research, Dental Clinics, Dental Prospects, 4, 110–114.
Radeghieri, A., Alacqua, S., Zendrini, A., Previcini, V., Todaro, F., Martini, G., Ricotta, D., & Bergese, P. (2022). Active antithrombin glycoforms are selectively physiosorbed on plasma extracellular vesicles. Journal of Extracellular Biology, 1, e57.
Ragni, E., Perucca Orfei, C., De Luca, P., Lugano, G., Viganò, M., Colombini, A., Valli, F., Zacchetti, D., Bollati, V., & de Girolamo, L. (2019). Interaction with hyaluronan matrix and miRNA cargo as contributors for in vitro potential of mesenchymal stem cell-derived extracellular vesicles in a model of human osteoarthritic synoviocytes. Stem Cell Research & Therapy, 10, 109.
Rahman, M. M., Shimizu, K., Yamauchi, M., Takase, H., Ugawa, S., Okada, A., & Inoshima, Y. (2019). Acidification effects on isolation of extracellular vesicles from bovine milk. PLoS ONE, 14, e0222613.
Raj, A., Kato, C., Witek, H. A., & Hamaguchi, H. (2020). Toward standardization of Raman spectroscopy: Accurate wavenumber and intensity calibration using rotational Raman spectra of H-2, HD, D-2, and vibration-rotation spectrum of O-2. Journal of Raman Spectroscopy, 51, 2066–2082.
Ramirez-Garrastacho, M., Bajo-Santos, C., Line, A., Martens-Uzunova, E. S., de la Fuente, J. M., Moros, M., Soekmadji, C., Tasken, K. A., & Llorente, A. (2022). Extracellular vesicles as a source of prostate cancer biomarkers in liquid biopsies: A decade of research. British Journal of Cancer, 126, 331–350.
Rao, P., Benito, E., & Fischer, A. (2013). MicroRNAs as biomarkers for CNS disease. Frontiers in Molecular Neuroscience, 6, 39.
Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., & Geuze, H. J. (1996). B lymphocytes secrete antigen-presenting vesicles. Journal of Experimental Medicine, 183, 1161–1172.
Razzauti, A., & Laurent, P. (2021). Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons. Elife, 10, e67670.
Ridder, K., Keller, S., Dams, M., Rupp, A. K., Schlaudraff, J., del Turco, D., Starmann, J., Macas, J., Karpova, D., Devraj, K., Depboylu, C., Landfried, B., Arnold, B., Plate, K. H., Höglinger, G., Sültmann, H., Altevogt, P., & Momma, S. (2014). Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation. Plos Biology, 12, e1001874.
Ridolfi, A., Brucale, M., Montis, C., Caselli, L., Paolini, L., Borup, A., Boysen, A. T., Loria, F., van Herwijnen, M. J. C., Kleinjan, M., Nejsum, P., Zarovni, N., Wauben, M. H. M., Berti, D., Bergese, P., & Valle, F. (2020). AFM-based high-throughput nanomechanical screening of single extracellular vesicles. Analytical Chemistry, 92, 10274–10282.
Ridolfi, A., Caselli, L., Baldoni, M., Montis, C., Mercuri, F., Berti, D., Valle, F., & Brucale, M. (2021). Stiffness of fluid and gel phase lipid nanovesicles: Weighting the contributions of membrane bending modulus and luminal pressurization. Langmuir, 37, 12027–12037.
Riekse, R. G., Li, G., Petrie, E. C., Leverenz, J. B., Vavrek, D., Vuletic, S., Albers, J. J., Montine, T. J., Lee, V. M., Lee, M., Seubert, P., Galasko, D., Schellenberg, G. D., Hazzard, W. R., & Peskind, E. R. (2006). Effect of statins on Alzheimer's disease biomarkers in cerebrospinal fluid. Journal of Alzheimer's Disease, 10, 399–406.
Rikkert, L. G., Nieuwland, R., Terstappen, L., & Coumans, F. A. W. (2019). Quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent. Journal of Extracellular Vesicles, 8, 1555419.
Roberts-Dalton, H. D., Cocks, A., Falcon-Perez, J. M., Sayers, E. J., Webber, J. P., Watson, P., Clayton, A., & Jones, A. T. (2017). Fluorescence labelling of extracellular vesicles using a novel thiol-based strategy for quantitative analysis of cellular delivery and intracellular traffic. Nanoscale, 9, 13693–13706.
Rodrigues, A. D., van Dyk, M., Sorich, M. J., Fahmy, A., Useckaite, Z., Newman, L. A., Kapetas, A. J., Mounzer, R., Wood, L. S., Johnson, J. G., & Rowland, A. (2021). Exploring the use of serum-derived small extracellular vesicles as liquid biopsy to study the induction of hepatic cytochromes P450 and organic anion transporting polypeptides. Clinical Pharmacology & Therapeutics, 110, 248–258.
Rojalin, T., Koster, H. J., Liu, J., Mizenko, R. R., Tran, D., Wachsmann-Hogiu, S., & Carney, R. P. (2020). Hybrid nanoplasmonic porous biomaterial scaffold for liquid biopsy diagnostics using extracellular vesicles. ACS Sensors, 5, 2820–2833.
Romanò, S., Di Giacinto, F., Primiano, A., Gervasoni, J., Mazzini, A., Papi, M., Urbani, A., Serafino, A., De Spirito, M., Krasnowska, E. K., & Ciasca, G. (2022). Label-free spectroscopic characterization of exosomes reveals cancer cell differentiation. Analytica Chimica Acta, 1192, 339359.
Rostgaard, N., Olsen, M. H., Ottenheijm, M., Drici, L., Simonsen, A. H., Plomgaard, P., Gredal, H., Poulsen, H. H., Zetterberg, H., Blennow, K., Hasselbalch, S. G., MacAulay, N., & Juhler, M. (2023). Differential proteomic profile of lumbar and ventricular cerebrospinal fluid. Fluids Barriers CNS, 20, 6.
Roux, Q., Van Deun, J., Dedeyne, S., & Hendrix, A. (2020). The EV-TRACK summary add-on: Integration of experimental information in databases to ensure comprehensive interpretation of biological knowledge on extracellular vesicles. Journal of Extracellular Vesicles, 9, 1699367.
Royo, F., Cossío, U., Ruiz de Angulo, A., Llop, J., & Falcon-Perez, J. M. (2019). Modification of the glycosylation of extracellular vesicles alters their biodistribution in mice. Nanoscale, 11, 1531–1537.
Royo, F., Théry, C., Falcón-Pérez, J. M., Nieuwland, R., & Witwer, K. W. (2020). Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization subcommittee. Cells, 9, 1955.
Rufino-Ramos, D., Lule, S., Mahjoum, S., Ughetto, S., Cristopher Bragg, D., Pereira de Almeida, L., Breakefield, X. O., & Breyne, K. (2022). Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargo. Biomaterials, 281, 121366.
Russell, A. E., Sneider, A., Witwer, K. W., Bergese, P., Bhattacharyya, S. N., Cocks, A., Cocucci, E., Erdbrügger, U., Falcon-Perez, J. M., Freeman, D. W., Gallagher, T. M., Hu, S., Huang, Y., Jay, S. M., Kano, S. I., Lavieu, G., Leszczynska, A., Llorente, A. M., Lu, Q., … Vader, P. (2019). Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop. Journal of Extracellular Vesicles, 8, 1684862.
Russell, J. C., Kim, T. K., Noori, A., Merrihew, G. E., Robbins, J. E., Golubeva, A., Wang, K., MacCoss, M. J., & Kaeberlein, M. (2020). Composition of Caenorhabditis elegans extracellular vesicles suggests roles in metabolism, immunity, and aging. Geroscience, 42, 1133–1145.
Rust, M. J., Bates, M., & Zhuang, X. W. (2006). Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nature Methods, 3, 793–795.
Rüwald, J. M., Randau, T. M., Hilgers, C., Masson, W., Irsen, S., Eymael, R. L., Kohlhof, H., Gravius, S., Burger, C., Wirtz, D. C., & Schildberg, F. A. (2020). Extracellular vesicle isolation and characterization from periprosthetic joint synovial fluid in revision total joint arthroplasty. Journal of Clinical Medicine, 9, 516.
Saari, H., Pusa, R., Marttila, H., Yliperttula, M., & Laitinen, S. (2023). Development of tandem cation exchange chromatography for high purity extracellular vesicle isolation: The effect of ligand steric availability. Journal of Chromatography A, 1707, 464293.
Saari, H., Turunen, T., Lõhmus, A., Turunen, M., Jalasvuori, M., Butcher, S. J., Ylä-Herttuala, S., Viitala, T., Cerullo, V., Siljander, P. R. M., & Yliperttula, M. (2020). Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery. Journal of Extracellular Vesicles, 9, 1747206.
Saludes, J. P., Morton, L. A., Ghosh, N., Beninson, L. A., Chapman, E. R., Fleshner, M., & Yin, H. (2012). Detection of highly curved membrane surfaces using a cyclic peptide derived from synaptotagmin-I. Acs Chemical Biology, 7, 1629–1635.
Sanada, T., Hirata, Y., Naito, Y., Yamamoto, N., Kikkawa, Y., Ishida, Y., Yamasaki, C., Tateno, C., Ochiya, T., & Kohara, M. (2017). Transmission of HBV DNA mediated by ceramide-triggered extracellular vesicles. Cellular and Molecular Gastroenterology and Hepatology, 3, 272–283.
Sandau, U. S., Duggan, E., Shi, X., Smith, S. J., Huckans, M., Schutzer, W. E., Loftis, J. M., Janowsky, A., Nolan, J. P., & Saugstad, J. A. (2020). Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study. Journal of Extracellular Vesicles, 10, e12028.
Sansone, P., Savini, C., Kurelac, I., Chang, Q., Amato, L. B., Strillacci, A., Stepanova, A., Iommarini, L., Mastroleo, C., Daly, L., Galkin, A., Thakur, B. K., Soplop, N., Uryu, K., Hoshino, A., Norton, L., Bonafé, M., Cricca, M., Gasparre, G., … Bromberg, J. (2017). Packaging and transfer of mitochondrial DNA via exosomes regulate escape from dormancy in hormonal therapy-resistant breast cancer. PNAS, 114, E9066–E9075.
Santoro, J., Mukhopadhya, A., Oliver, C., Brodkorb, A., Giblin, L., & O'Driscoll, L. (2023). An investigation of extracellular vesicles in bovine colostrum, first milk and milk over the lactation curve. Food Chemistry, 401, 134029.
Schioppo, T., Ubiali, T., Ingegnoli, F., Bollati, V., & Caporali, R. (2021). The role of extracellular vesicles in rheumatoid arthritis: a systematic review. Clinical Rheumatology, 40, 3481–3497.
Scott, A., Sueiro Ballesteros, L., Bradshaw, M., Tsuji, C., Power, A., Lorriman, J., Love, J., Paul, D., Herman, A., Emanueli, C., & Richardson, R. J. (2021). In vivo characterization of endogenous cardiovascular extracellular vesicles in larval and adult zebrafish. Arteriosclerosis, Thrombosis, and Vascular Biology, 41, 2454–2468.
Shah, S. S., Ebberson, J., Kestenbaum, L. A., Hodinka, R. L., & Zorc, J. J. (2011). Age-specific reference values for cerebrospinal fluid protein concentration in neonates and young infants. Journal of Hospital Medicine, 6, 22–27.
Sharma, A., Mariappan, M., Appathurai, S., & Hegde, R. S. (2010). In vitro dissection of protein translocation into the mammalian endoplasmic reticulum. Methods in Molecular Biology, 619, 339–363.
Sharma, S., Gillespie, B. M., Palanisamy, V., & Gimzewski, J. K. (2011). Quantitative nanostructural and single-molecule force spectroscopy biomolecular analysis of human-saliva-derived exosomes. Langmuir, 27, 14394–14400.
Sharma, S., LeClaire, M., & Gimzewski, J. K. (2018). Ascent of atomic force microscopy as a nanoanalytical tool for exosomes and other extracellular vesicles. Nanotechnology, 29, 132001.
Sharma, S., LeClaire, M., Wohlschlegel, J., & Gimzewski, J. (2020). Impact of isolation methods on the biophysical heterogeneity of single extracellular vesicles. Scientific Reports, 10, 13327.
Shekari, F., Alibhai, F. J., Baharvand, H., Börger, V., Bruno, S., Davies, O., Giebel, B., Gimona, M., Salekdeh, G. H., Martin-Jaular, L., Mathivanan, S., Nelissen, I., Hoen, E. N.-T., O'Driscoll, L., Perut, F., Pluchino, S., Pocsfalvi, G., Salomon, C., Soekmadji, C., … Nieuwland, R. (2023). Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters. Journal of Extracellular Biology, 2, e115.
Shen, S., Shen, Z., Wang, C., Wu, X., Wang, L., Ye, L., Zhang, S., & Cheng, X. (2023). Effects of lysate/tissue storage at −80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins. Iscience, 26, 106521.
Shlomovitz, I., Erlich, Z., Arad, G., Edry-Botzer, L., Zargarian, S., Cohen, H., Manko, T., Ofir-Birin, Y., Cooks, T., Regev-Rudzki, N., & Gerlic, M. (2021). Proteomic analysis of necroptotic extracellular vesicles. Cell Death & Disease, 12, 1059.
Silva, A. M., Lázaro-Ibáñez, E., Gunnarsson, A., Dhande, A., Daaboul, G., Peacock, B., Osteikoetxea, X., Salmond, N., Friis, K. P., Shatnyeva, O., & Dekker, N. (2021). Quantification of protein cargo loading into engineered extracellular vesicles at single-vesicle and single-molecule resolution. Journal of Extracellular Vesicles, 10, e12130.
Simonsen, J. B. (2017). What are we looking at? Extracellular vesicles, lipoproteins, or both? Circulation Research, 121, 920–922.
Simonsen, J. B. (2019). Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies. Journal of Extracellular Vesicles, 8, 1582237.
Singh, P., Szigyártó, I. C., Ricci, M., Zsila, F., Juhász, T., Mihály, J., Bősze, S., Bulyáki, É., Kardos, J., Kitka, D., Varga, Z., & Beke-Somfai, T. (2020). Membrane active peptides remove surface adsorbed protein corona from extracellular vesicles of red blood cells. Frontiers in Chemistry, 8, 703.
Sitar, S., Kejžar, A., Pahovnik, D., Kogej, K., Tušek-Žnidarič, M., Lenassi, M., & Žagar, E. (2015). Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation. Analytical Chemistry, 87, 9225–9233.
Skliar, M., Chernyshev, V. S., Belnap, D. M., Sergey, G. V., Al-Hakami, S. M., Bernard, P. S., Stijleman, I. J., & Rachamadugu, R. (2018). Membrane proteins significantly restrict exosome mobility. Biochemical and Biophysical Research Communications, 501, 1055–1059.
Skotland, T., Iversen, T. G., Llorente, A., & Sandvig, K. (2022). Biodistribution, pharmacokinetics and excretion studies of intravenously injected nanoparticles and extracellular vesicles: Possibilities and challenges. Advanced Drug Delivery Reviews, 186, 114326.
Smith, E., & Dent, G. (2005). Modern raman spectroscopy: A practical approach. 1–210.
Smith, Z. J., Lee, C., Rojalin, T., Carney, R. P., Hazari, S., Knudson, A., Lam, K., Saari, H., Ibanez, E. L., Viitala, T., Laaksonen, T., Yliperttula, M., & Wachsmann-Hogiu, S. (2015). Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content. Journal of Extracellular Vesicles, 4, 28533.
Sódar, B. W., Kittel, Á., Pálóczi, K., Vukman, K. V., Osteikoetxea, X., Szabó-Taylor, K., Németh, A., Sperlágh, B., Baranyai, T., Giricz, Z., Wiener, Z., Turiák, L., Drahos, L., Pállinger, É., Vékey, K., Ferdinandy, P., Falus, A., & Buzás, E. I. (2016). Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection. Scientific Reports, 6, 24316.
Sodar, B. W., Kovacs, A., Visnovitz, T., Pallinger, E., Vekey, K., Pocsfalvi, G., Turiak, L., & Buzas, E. I. (2017). Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease. Expert Review of Proteomics, 14, 1073–1090.
Soekmadji, C., Hill, A. F., Wauben, M. H., Buzás, E. I., Di Vizio, D., Gardiner, C., Lötvall, J., Sahoo, S., & Witwer, K. W. (2018). Towards mechanisms and standardization in extracellular vesicle and extracellular RNA studies: Results of a worldwide survey. Journal of Extracellular Vesicles, 7, 1535745.
Somiya, M., & Kuroda, S. (2021a). Real-time luminescence assay for cytoplasmic cargo delivery of extracellular vesicles. Analytical Chemistry, 93, 5612–5620.
Somiya, M., & Kuroda, S. (2021b). Reporter gene assay for membrane fusion of extracellular vesicles. Journal of Extracellular Vesicles, 10, e12171.
Somiya, M., Yoshioka, Y., & Ochiya, T. (2018). Biocompatibility of highly purified bovine milk-derived extracellular vesicles. Journal of Extracellular Vesicles, 7, 1440132.
Sorkin, R., Huisjes, R., Bošković, F., Vorselen, D., Pignatelli, S., Ofir-Birin, Y., Freitas Leal, J. K., Schiller, J., Mullick, D., Roos, W. H., Bosman, G., Regev-Rudzki, N., Schiffelers, R. M., & Wuite, G. J. L. (2018). Nanomechanics of extracellular vesicles reveals vesiculation pathways. Small, 14, e1801650.
Stam, J., Bartel, S., Bischoff, R., & Wolters, J. C. (2021). Isolation of extracellular vesicles with combined enrichment methods. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1169, 122604.
Steenbeek, S. C., Pham, T. V., de Ligt, J., Zomer, A., Knol, J. C., Piersma, S. R., Schelfhorst, T., Huisjes, R., Schiffelers, R. M., Cuppen, E., Jimenez, C. R., & van Rheenen, J. (2018). Cancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles. Embo Journal, 37, e98357.
Stetefeld, J., McKenna, S. A., & Patel, T. R. (2016). Dynamic light scattering: a practical guide and applications in biomedical sciences. Biophysical Reviews, 8, 409–427.
Stoner, S. A., Duggan, E., Condello, D., Guerrero, A., Turk, J. R., Narayanan, P. K., & Nolan, J. P. (2016). High sensitivity flow cytometry of membrane vesicles. Cytometry Part A: the journal of the International Society for Analytical Cytology, 89, 196–206.
Sung, B. H., Ketova, T., Hoshino, D., Zijlstra, A., & Weaver, A. M. (2015). Directional cell movement through tissues is controlled by exosome secretion. Nature Communications, 6, 7164.
Sung, B. H., & Weaver, A. M. (2017). Exosome secretion promotes chemotaxis of cancer cells. Cell Adhesion & Migration, 11, 187–195.
Suwatthanarak, T., Thiodorus, I. A., Tanaka, M., Shimada, T., Takeshita, D., Yasui, T., Baba, Y., & Okochi, M. (2021). Microfluidic-based capture and release of cancer-derived exosomes via peptide-nanowire hybrid interface. Lab on A Chip, 21, 597–607.
Taha, E. A., Sogawa, C., Okusha, Y., Kawai, H., Oo, M. W., Elseoudi, A., Lu, Y., Nagatsuka, H., Kubota, S., Satoh, A., Okamoto, K., & Eguchi, T. (2020). Knockout of MMP3 weakens solid tumor organoids and cancer extracellular vesicles. Cancers (Basel), 12, 1260.
Taher, S., Borja, Y., Cabanela, L., Costers, V. J., Carson-Marino, M., Bailes, J. C., Dhar, B., Beckworth, M. T., Rabaglino, M. B., Post Uiterweer, E. D., & Conrad, K. P. (2019). Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: Trophoblast expression and signaling. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 316, R628–R639.
Takov, K., Yellon, D. M., & Davidson, S. M. (2017). Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes. Journal of Extracellular Vesicles, 6, 1388731.
Tassetto, M., Kunitomi, M., & Andino, R. (2017). Circulating immune cells mediate a systemic RNAi-based adaptive antiviral response in drosophila. Cell, 169, 314–325.e13.
Taylor, C. F., Paton, N. W., Lilley, K. S., Binz, P. A., Julian R. K., Jr, Jones, A. R., Zhu, W., Apweiler, R., Aebersold, R., Deutsch, E. W., Dunn, M. J., Heck, A. J., Leitner, A., Macht, M., Mann, M., Martens, L., Neubert, T. A., Patterson, S. D., Ping, P., … Hermjakob, H. (2007). The minimum information about a proteomics experiment (MIAPE). Nature Biotechnology, 25, 887–893.
Ter-Ovanesyan, D., Gilboa, T., Budnik, B., Nikitina, A., Whiteman, S., Lazarovits, R., Trieu, W., Kalish, D., Church, G. M., & Walt, D. R. (2023). Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins. Elife, 12, e86394.
Ter-Ovanesyan, D., Norman, M., Lazarovits, R., Trieu, W., Lee, J. H., Church, G. M., & Walt, D. R. (2021). Framework for rapid comparison of extracellular vesicle isolation methods. Elife, 10, e70725.
Teunissen, C. E., Petzold, A., Bennett, J. L., Berven, F. S., Brundin, L., Comabella, M., Franciotta, D., Frederiksen, J. L., Fleming, J. O., Furlan, R., Hintzen, R. Q., Hughes, S. G., Johnson, M. H., Krasulova, E., Kuhle, J., Magnone, M. C., Rajda, C., Rejdak, K., Schmidt, H. K., … Deisenhammer, F. (2009). A consensus protocol for the standardization of cerebrospinal fluid collection and biobanking. Neurology, 73, 1914–1922.
Thakur, B. K., Zhang, H., Becker, A., Matei, I., Huang, Y., Costa-Silva, B., Zheng, Y., Hoshino, A., Brazier, H., Xiang, J., Williams, C., Rodriguez-Barrueco, R., Silva, J. M., Zhang, W., Hearn, S., Elemento, O., Paknejad, N., Manova-Todorova, K., Welte, K., … Lyden, D. (2014). Double-stranded DNA in exosomes: A novel biomarker in cancer detection. Cell Research, 24, 766–769.
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006). Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Current Protocols in Cell Biology, Chapter 3, Unit 3.22.
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin-Smith, G. K., Ayre, D. C., Bach, J. M., Bachurski, D., Baharvand, H., Balaj, L., Baldacchino, S., Bauer, N. N., Baxter, A. A., Bebawy, M., … Zuba-Surma, E. K. (2018). Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles, 7(1), 1535750. https://doi.org/10.1080/20013078.2018.1535750
Thomas, R. E., Vincow, E. S., Merrihew, G. E., MacCoss, M. J., Davis, M. Y., & Pallanck, L. J. (2018). Glucocerebrosidase deficiency promotes protein aggregation through dysregulation of extracellular vesicles. Plos Genetics, 14, e1007694.
Tian, T., Wang, Y., Wang, H., Zhu, Z., & Xiao, Z. (2010). Visualizing of the cellular uptake and intracellular trafficking of exosomes by live-cell microscopy. Journal of Cellular Biochemistry, 111, 488–496.
Tian, Y., Gong, M., Hu, Y., Liu, H., Zhang, W., Zhang, M., Hu, X., Aubert, D., Zhu, S., Wu, L., & Yan, X. (2020). Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry. Journal of Extracellular Vesicles, 9, 1697028.
Tian, Y., Ma, L., Gong, M., Su, G., Zhu, S., Zhang, W., Wang, S., Li, Z., Chen, C., Li, L., Wu, L., & Yan, X. (2018). Protein profiling and sizing of extracellular vesicles from colorectal cancer patients via flow cytometry. ACS Nano, 12, 671–680.
Toda, H., Diaz-Varela, M., Segui-Barber, J., Roobsoong, W., Baro, B., Garcia-Silva, S., Galiano, A., Gualdron-Lopez, M., Almeida, A. C. G., Brito, M. A. M., de Melo, G. C., Aparici-Herraiz, I., Castro-Cavadia, C., Monteiro, W. M., Borras, E., Sabido, E., Almeida, I. C., Chojnacki, J., Martinez-Picado, J., … del Portillo, H. A. (2020). Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence. Nature Communications, 11, 2761.
Tóth, E. Á., Turiák, L., Visnovitz, T., Cserép, C., Mázló, A., Sódar, B. W., Försönits, A. I., Petővári, G., Sebestyén, A., Komlósi, Z., Drahos, L., Kittel, Á., Nagy, G., Bácsi, A., Dénes, Á., Gho, Y. S., Szabó-Taylor, K. É., & Buzás, E. I. (2021). Formation of a protein corona on the surface of extracellular vesicles in blood plasma. Journal of Extracellular Vesicles, 10, e12140.
Toyofuku, M., Schild, S., Kaparakis-Liaskos, M., & Eberl, L. (2023). Composition and functions of bacterial membrane vesicles. Nature Reviews Microbiology, 21, 415–430.
Trajkovic, K., Hsu, C., Chiantia, S., Rajendran, L., Wenzel, D., Wieland, F., Schwille, P., Brügger, B., & Simons, M. (2008). Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science, 319, 1244–1247.
Trenkenschuh, E., Richter, M., Heinrich, E., Koch, M., Fuhrmann, G., & Friess, W. (2022). Enhancing the stabilization potential of lyophilization for extracellular vesicles. Advanced Healthcare Materials, 11, e2100538.
Tsai, Y. W., Sung, H. H., Li, J. C., Yeh, C. Y., Chen, P. Y., Cheng, Y. J., Chen, C. H., Tsai, Y. C., & Chien, C. T. (2019). Glia-derived exosomal miR-274 targets Sprouty in trachea and synaptic boutons to modulate growth and responses to hypoxia. PNAS, 116, 24651–24661.
Tulkens, J., Vergauwen, G., Van Deun, J., Geeurickx, E., Dhondt, B., Lippens, L., De Scheerder, M. A., Miinalainen, I., Rappu, P., De Geest, B. G., Vandecasteele, K., Laukens, D., Vandekerckhove, L., Denys, H., Vandesompele, J., De Wever, O., & Hendrix, A. (2020). Increased levels of systemic LPS-positive bacterial extracellular vesicles in patients with intestinal barrier dysfunction. Gut, 69, 191–193.
Turner, L., Bitto, N. J., Steer, D. L., Lo, C., D'Costa, K., Ramm, G., Shambrook, M., Hill, A. F., Ferrero, R. L., & Kaparakis-Liaskos, M. (2018). Helicobacter pylori outer membrane vesicle size determines their mechanisms of host cell entry and protein content. Frontiers in Immunology, 9, 1466.
Vagner, T., Spinelli, C., Minciacchi, V. R., Balaj, L., Zandian, M., Conley, A., Zijlstra, A., Freeman, M. R., Demichelis, F., De, S., Posadas, E. M., Tanaka, H., & Di Vizio, D. (2018). Large extracellular vesicles carry most of the tumour DNA circulating in prostate cancer patient plasma. Journal of Extracellular Vesicles, 7, 1505403.
Valcz, G., Buzas, E. I., Kittel, A., Krenacs, T., Visnovitz, T., Spisak, S., Torok, G., Homolya, L., Zsigrai, S., Kiszler, G., Antalffy, G., Paloczi, K., Szallasi, Z., Szabo, V., Sebestyen, A., Solymosi, N., Kalmar, A., Dede, K., Lorincz, P., … Molnar, B. (2019). En bloc release of MVB-like small extracellular vesicle clusters by colorectal carcinoma cells. Journal of Extracellular Vesicles, 8(1), 1596668.
Valkov, N., Das, A., Tucker, N. R., Li, G., Salvador, A. M., Chaffin, M. D., Pereira de Oliveira Junior, G., Kur, I., Gokulnath, P., Ziegler, O., Yeri, A., Lu, S., Khamesra, A., Xiao, C., Rodosthenous, R., Srinivasan, S., Toxavidis, V., Tigges, J., Laurent, L. C., … Das, S. (2021). SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart. Life Science Alliance, 4, e202101048.
van der Pol, E., Coumans, F. A., Grootemaat, A. E., Gardiner, C., Sargent, I. L., Harrison, P., Sturk, A., van Leeuwen, T. G., & Nieuwland, R. (2014). Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. Journal of Thrombosis and Haemostasis, 12, 1182–1192.
van der Pol, E., Coumans, F. A., Sturk, A., Nieuwland, R., & van Leeuwen, T. G. (2014). Refractive index determination of nanoparticles in suspension using nanoparticle tracking analysis. Nano Letters, 14, 6195–6201.
van der Pol, E., de Rond, L., Coumans, F. A. W., Gool, E. L., Boing, A. N., Sturk, A., Nieuwland, R., & van Leeuwen, T. G. (2018). Absolute sizing and label-free identification of extracellular vesicles by flow cytometry. Nanomedicine, 14, 801–810.
van der Pol, E., Sturk, A., van Leeuwen, T., Nieuwland, R., Coumans, F., & Isth-Ssc-Vb Working group. (2018). Standardization of extracellular vesicle measurements by flow cytometry through vesicle diameter approximation. Journal of Thrombosis and Haemostasis, 16, 1236–1245.
van der Pol, E., Welsh, J. A., & Nieuwland, R. (2022). Minimum information to report about a flow cytometry experiment on extracellular vesicles: Communication from the ISTH SSC subcommittee on vascular biology. Journal of Thrombosis and Haemostasis, 20, 245–251.
van Deun, J., Jo, A., Li, H., Lin, H. Y., Weissleder, R., Im, H., & Lee, H. (2020). Integrated dual-mode chromatography to enrich extracellular vesicles from plasma. Advanced Biology, 4, e1900310.
van Royen, M. E., Soekmadji, C., Grange, C., Webber, J. P., Tertel, T., Droste, M., Buescher, A., Giebel, B., Jenster, G. W., Llorente, A., Blijdorp, C. J., Burger, D., Erdbrügger, U., & Martens-Uzunova, E. S. (2023). The quick reference card “Storage of urinary EVs” – A practical guideline tool for research and clinical laboratories. Journal of Extracellular Vesicles, 12, e12286.
Vella, L. J., Scicluna, B. J., Cheng, L., Bawden, E. G., Masters, C. L., Ang, C. S., Willamson, N., McLean, C., Barnham, K. J., & Hill, A. F. (2017). A rigorous method to enrich for exosomes from brain tissue. Journal of Extracellular Vesicles, 6, 1348885.
Vergauwen, G., Dhondt, B., Van Deun, J., De Smedt, E., Berx, G., Timmerman, E., Gevaert, K., Miinalainen, I., Cocquyt, V., Braems, G., Van den Broecke, R., Denys, H., De Wever, O., & Hendrix, A. (2017). Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research. Scientific Reports, 7, 2704.
Vergauwen, G., Tulkens, J., Pinheiro, C., Avila Cobos, F., Dedeyne, S., De Scheerder, M. A., Vandekerckhove, L., Impens, F., Miinalainen, I., Braems, G., Gevaert, K., Mestdagh, P., Vandesompele, J., Denys, H., De Wever, O., & Hendrix, A. (2021). Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions. Journal of Extracellular Vesicles, 10, e12122.
Verweij, F. J., Balaj, L., Boulanger, C. M., Carter, D. R. F., Compeer, E. B., D'Angelo, G., El Andaloussi, S., Goetz, J. G., Gross, J. C., Hyenne, V., Krämer-Albers, E. M., Lai, C. P., Loyer, X., Marki, A., Momma, S., Nolte-’t Hoen, E. N. M., Pegtel, D. M., Peinado, H., Raposo, G., … van Niel, G. (2021). The power of imaging to understand extracellular vesicle biology in vivo. Nature Methods, 18(9), 1013–1026.
Verweij, F. J., Revenu, C., Arras, G., Dingli, F., Loew, D., Pegtel, D. M., Follain, G., Allio, G., Goetz, J. G., Zimmermann, P., Herbomel, P., Del Bene, F., Raposo, G., & van Niel, G. (2019). Live tracking of inter-organ communication by endogenous exosomes in vivo. Developmental Cell, 48, 573–589.e4.
Verwilt, J., Trypsteen, W., Van Paemel, R., De Preter, K., Giraldez, M. D., Mestdagh, P., & Vandesompele, J. (2020). When DNA gets in the way: A cautionary note for DNA contamination in extracellular RNA-seq studies. PNAS, 117, 18934–18936.
Vestad, B., Llorente, A., Neurauter, A., Phuyal, S., Kierulf, B., Kierulf, P., Skotland, T., Sandvig, K., Haug, K. B. F., & Ovstebo, R. (2017). Size and concentration analyses of extracellular vesicles by nanoparticle tracking analysis: A variation study. Journal of Extracellular Vesicles, 6, 1344087.
Visnovitz, T., Osteikoetxea, X., Sodar, B. W., Mihaly, J., Lorincz, P., Vukman, K. V., Toth, E. A., Koncz, A., Szekacs, I., Horvath, R., Varga, Z., & Buzas, E. I. (2019). An improved 96 well plate format lipid quantification assay for standardisation of experiments with extracellular vesicles. Journal of Extracellular Vesicles, 8, 1565263.
Vorselen, D., MacKintosh, F. C., Roos, W. H., & Wuite, G. J. (2017). Competition between bending and internal pressure governs the mechanics of fluid nanovesicles. ACS Nano, 11, 2628–2636.
Vorselen, D., van Dommelen, S. M., Sorkin, R., Piontek, M. C., Schiller, J., Döpp, S. T., Kooijmans, S. A. A., van Oirschot, B. A., Versluijs, B. A., Bierings, M. B., van Wijk, R., Schiffelers, R. M., Wuite, G. J. L., & Roos, W. H. (2018). The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis. Nature Communications, 9, 4960.
Walker, J. G. (2012). Improved nano-particle tracking analysis. Measurement Science and Technology, 23, 065605.
Walsh, R. B., Dresselhaus, E. C., Becalska, A. N., Zunitch, M. J., Blanchette, C. R., Scalera, A. L., Lemos, T., Lee, S. M., Apiki, J., Wang, S., Isaac, B., Yeh, A., Koles, K., & Rodal, A. A. (2021). Opposing functions for retromer and Rab11 in extracellular vesicle traffic at presynaptic terminals. Journal of Cell Biology, 220, e202012034.
Wang, C., Ding, Q., Plant, P., Basheer, M., Yang, C., Tawedrous, E., Krizova, A., Boulos, C., Farag, M., Cheng, Y., & Yousef, G. M. (2019). Droplet digital PCR improves urinary exosomal miRNA detection compared to real-time PCR. Clinical Biochemistry, 67, 54–59.
Wang, J., Kaletsky, R., Silva, M., Williams, A., Haas, L. A., Androwski, R. J., Landis, J. N., Patrick, C., Rashid, A., Santiago-Martinez, D., Gravato-Nobre, M., Hodgkin, J., Hall, D. H., Murphy, C. T., & Barr, M. M. (2015). Cell-specific transcriptional profiling of ciliated sensory neurons reveals regulators of behavior and extracellular vesicle biogenesis. Current Biology, 25, 3232–3238.
Wang, J., Silva, M., Haas, L. A., Morsci, N. S., Nguyen, K. C., Hall, D. H., & Barr, M. M. (2014). C. elegans ciliated sensory neurons release extracellular vesicles that function in animal communication. Current Biology, 24, 519–525.
Wang, X., Shen, H., Zhangyuan, G., Huang, R., Zhang, W., He, Q., Jin, K., Zhuo, H., Zhang, Z., Wang, J., Sun, B., & Lu, X. (2018). 14-3-3zeta delivered by hepatocellular carcinoma-derived exosomes impaired anti-tumor function of tumor-infiltrating T lymphocytes. Cell Death & Disease, 9, 159.
Wang, Z. T., Li, K. Y., Tan, C. C., Xu, W., Shen, X. N., Cao, X. P., Wang, P., Bi, Y. L., Dong, Q., Tan, L., & Yu, J. T. (2021). Associations of alcohol consumption with cerebrospinal fluid biomarkers of Alzheimer's disease pathology in cognitively intact older adults: The CABLE Study. Journal of Alzheimer's Disease, 82, 1045–1054.
Wehman, A. M., Poggioli, C., Schweinsberg, P., Grant, B. D., & Nance, J. (2011). The P4-ATPase TAT-5 inhibits the budding of extracellular vesicles in C. elegans embryos. Current Biology, 21, 1951–1959.
Welsh, J. A., Killingsworth, B., Kepley, J., Traynor, T., Cook, S., Savage, J., Marte, J., Lee, M., Maeng, H. M., Pleet, M. L., Magana, S., Gorgens, A., Maire, C. L., Lamszus, K., Ricklefs, F. L., Merino, M. J., Linehan, W. M., Greten, T., Cooks, T., … Jones, J. C. (2022). MPAPASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays. Cell Reports Methods, 2, 100136.
Welsh, J. A., Arkesteijn, G. J. A., Bremer, M., Cimorelli, M., Dignat-George, F., Giebel, B., Görgens, A., Hendrix, A., Kuiper, M., Lacroix, R., Lannigan, J., van Leeuwen, T. G., Lozano-Andrés, E., Rao, S., Robert, S., de Rond, L., Tang, V. A., Tertel, T., Yan, X., … van der Pol, E. (2023). A compendium of single extracellular vesicle flow cytometry. Journal of Extracellular Vesicles, 12, e12299.
Welsh, J. A., Horak, P., Wilkinson, J. S., Ford, V. J., Jones, J. C., Smith, D., Holloway, J. A., & Englyst, N. A. (2020). FCMPASS software aids extracellular vesicle light scatter standardization. Cytometry Part A: The Journal of the International Society for Analytical Cytology, 97, 569–581.
Welsh, J. A., Jones, J. C., & Tang, V. A. (2020). Fluorescence and light scatter calibration allow comparisons of small particle data in standard units across different flow cytometry platforms and detector settings. Cytometry Part A: The Journal of the International Society for Analytical Cytology, 97, 592–601.
Welsh, J. A., Killingsworth, B., Kepley, J., Traynor, T., McKinnon, K., Savage, J., Appel, D., Aldape, K., Camphausen, K., Berzofsky, J. A., Ivanov, A. R., Ghiran, I. H., & Jones, J. C. (2021). A simple, high-throughput method of protein and label removal from extracellular vesicle samples. Nanoscale, 13, 3737–3745.
Welsh, J. A., Tang, V. A., van der Pol, E., & Görgens, A. (2021). MIFlowCyt-EV: The next chapter in the reporting and reliability of single extracellular vesicle flow cytometry experiments. Cytometry Part A: The Journal of the International Society for Analytical Cytology, 99, 365–368.
Welsh, J. A., Van Der Pol, E., Arkesteijn, G. J. A., Bremer, M., Brisson, A., Coumans, F., Dignat-George, F., Duggan, E., Ghiran, I., Giebel, B., Gorgens, A., Hendrix, A., Lacroix, R., Lannigan, J., Libregts, S., Lozano-Andres, E., Morales-Kastresana, A., Robert, S., de Rond, L., … Jones, J. C. (2020). MIFlowCyt-EV: A framework for standardized reporting of extracellular vesicle flow cytometry experiments. Journal of Extracellular Vesicles, 9, 1713526.
Welsh, J. A., van der Pol, E., Bettin, B. A., Carter, D. R. F., Hendrix, A., Lenassi, M., Langlois, M. A., Llorente, A., van de Nes, A. S., Nieuwland, R., Tang, V., Wang, L., Witwer, K. W., & Jones, J. C. (2020). Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size and concentration. Journal of Extracellular Vesicles, 9, 1816641.
Wen, S. W., Sceneay, J., Lima, L. G., Wong, C. S., Becker, M., Krumeich, S., Lobb, R. J., Castillo, V., Wong, K. N., Ellis, S., Parker, B. S., & Möller, A. (2016). The biodistribution and immune suppressive effects of breast cancer-derived exosomes. Cancer Research, 76, 6816–6827.
Whitehead, B., Wu, L., Hvam, M. L., Aslan, H., Dong, M., Dyrskjøt, L., Ostenfeld, M. S., Moghimi, S. M., & Howard, K. A. (2015). Tumour exosomes display differential mechanical and complement activation properties dependent on malignant state: Implications in endothelial leakiness. Journal of Extracellular Vesicles, 4, 29685.
Wiklander, O. P. B., Bostancioglu, R. B., Welsh, J. A., Zickler, A. M., Murke, F., Corso, G., Felldin, U., Hagey, D. W., Evertsson, B., Liang, X. M., Gustafsson, M. O., Mohammad, D. K., Wiek, C., Hanenberg, H., Bremer, M., Gupta, D., Bjornstedt, M., Giebel, B., Nordin, J. Z., … Gorgens, A. (2018). Systematic methodological evaluation of a multiplex bead-based flow cytometry assay for detection of extracellular vesicle surface signatures. Frontiers in Immunology, 9, 1326.
Wiklander, O. P., Nordin, J. Z., O'Loughlin, A., Gustafsson, Y., Corso, G., Mäger, I., Vader, P., Lee, Y., Sork, H., Seow, Y., Heldring, N., Alvarez-Erviti, L., Smith, C. I., Blanc, K. Le, Macchiarini, P., Jungebluth, P., Wood, M. J., & Andaloussi, S. E. (2015). Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting. Journal of Extracellular Vesicles, 4, 26316.
Willy, N. M., Colombo, F., Huber, S., Smith, A. C., Norton, E. G., Kural, C., & Cocucci, E. (2021). CALM supports clathrin-coated vesicle completion upon membrane tension increase. PNAS, 118, e2010438118.
Witwer, K. W., Buzas, E. I., Bemis, L. T., Bora, A., Lasser, C., Lotvall, J., Nolte-’t Hoen, E. N., Piper, M. G., Sivaraman, S., Skog, J., Thery, C., Wauben, M. H., & Hochberg, F. (2013). Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. Journal of Extracellular Vesicles, 2, 20360.
Witwer, K. W., Goberdhan, D. C., O'Driscoll, L., Théry, C., Welsh, J. A., Blenkiron, C., Buzás, E. I., di Vizio, D., Erdbrügger, U., Falcón-Pérez, J. M., Fu, Q. L., Hill, A. F., Lenassi, M., Lötvall, J., Nieuwland, R., Ochiya, T., Rome, S., Sahoo, S., & Zheng, L. (2021). Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles. Journal of Extracellular Vesicles, 10, e12182.
Wolf, M., Poupardin, R. W., Ebner-Peking, P., Andrade, A. C., Blöchl, C., Obermayer, A., Gomes, F. G., Vari, B., Maeding, N., Eminger, E., Binder, H. M., Raninger, A. M., Hochmann, S., Brachtl, G., Spittler, A., Heuser, T., Ofir, R., Huber, C. G., Aberman, Z., … Strunk, D. (2022). A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation. Journal of Extracellular Vesicles, 11, e12207.
Wombacher, R., Heidbreder, M., van de Linde, S., Sheetz, M. P., Heilemann, M., Cornish, V. W., & Sauer, M. (2010). Live-cell super-resolution imaging with trimethoprim conjugates. Nature Methods, 7, 717–719.
Wong, M., Schlaggar, B. L., Buller, R. S., Storch, G. A., & Landt, M. (2000). Cerebrospinal fluid protein concentration in pediatric patients: Defining clinically relevant reference values. Archives of Pediatrics & Adolescent Medicine, 154, 827–831.
Wong, V. (2007). Medication use as a confounding factor in the use of the cerebrospinal fluid tau/beta-amyloid42 ratio. Archives of Neurology, 64, 1357–1359.
Wood, C. R., Huang, K., Diener, D. R., & Rosenbaum, J. L. (2013). The cilium secretes bioactive ectosomes. Current Biology, 23, 906–911.
Woud, W. W., Hesselink, D. A., Hoogduijn, M. J., Baan, C. C., & Boer, K. (2022). Direct detection of circulating donor-derived extracellular vesicles in kidney transplant recipients. Scientific Reports, 12, 21973.
Wu, Y., Deng, W., & Klinke, D. J. 2nd (2015). Exosomes: Improved methods to characterize their morphology, RNA content, and surface protein biomarkers. Analyst, 140, 6631–6642.
Xiang, H., Jin, S., Tan, F., Xu, Y., Lu, Y., & Wu, T. (2021). Physiological functions and therapeutic applications of neutral sphingomyelinase and acid sphingomyelinase. Biomedicine & Pharmacotherapy, 139, 111610.
Xu, F., Laguna, L., & Sarkar, A. (2019). Aging-related changes in quantity and quality of saliva: Where do we stand in our understanding? Journal of Texture Studies, 50, 27–35.
Yang, C., Chalasani, G., Ng, Y. H., & Robbins, P. D. (2012). Exosomes released from Mycoplasma infected tumor cells activate inhibitory B cells. PLoS ONE, 7, e36138.
Yang, K., Jia, M., Cheddah, S., Zhang, Z., Wang, W., Li, X., Wang, Y., & Yan, C. (2022). Peptide ligand-SiO(2) microspheres with specific affinity for phosphatidylserine as a new strategy to isolate exosomes and application in proteomics to differentiate hepatic cancer. Bioactive Materials, 15, 343–354.
Ye, S., Li, W., Wang, H., Zhu, L., Wang, C., & Yang, Y. (2021). Quantitative nanomechanical analysis of small extracellular vesicles for tumor malignancy indication. Advanced Science, 8, e2100825.
Yelamanchili, S. V., Lamberty, B. G., Rennard, D. A., Morsey, B. M., Hochfelder, C. G., Meays, B. M., Levy, E., & Fox, H. S. (2015). MiR-21 in extracellular vesicles leads to neurotoxicity via TLR7 signaling in SIV neurological disease. Plos Pathogens, 11, e1005032.
Yerneni, S. S., Solomon, T., Smith, J., & Campbell, P. G. (2022). Radioiodination of extravesicular surface constituents to study the biocorona, cell trafficking and storage stability of extracellular vesicles. Biochimica et Biophysica Acta (BBA) - General Subjects, 1866, 130069.
You, J. S., Gelfanova, V., Knierman, M. D., Witzmann, F. A., Wang, M., & Hale, J. E. (2005). The impact of blood contamination on the proteome of cerebrospinal fluid. Proteomics, 5, 290–296.
Young, G., Hundt, N., Cole, D., Fineberg, A., Andrecka, J., Tyler, A., Olerinyova, A., Ansari, A., Marklund, E. G., Collier, M. P., Chandler, S. A., Tkachenko, O., Allen, J., Crispin, M., Billington, N., Takagi, Y., Sellers, J. R., Eichmann, C., Selenko, P., … Kukura, P. (2018). Quantitative mass imaging of single biological macromolecules. Science, 360, 423–427.
Yuana, Y., Koning, R. I., Kuil, M. E., Rensen, P. C., Koster, A. J., Bertina, R. M., & Osanto, S. (2013). Cryo-electron microscopy of extracellular vesicles in fresh plasma. Journal of Extracellular Vesicles, 2, https://doi.org/10.3402/jev.v2i0.21494
Zavan, L., Bitto, N. J., Johnston, E. L., Greening, D. W., & Kaparakis-Liaskos, M. (2019). Helicobacter pylori growth stage determines the size, protein composition, and preferential cargo packaging of outer membrane vesicles. Proteomics, 19, e1800209.
Zavan, L., Fang, H., Johnston, E. L., Whitchurch, C., Greening, D., Hill, A. F., & KaparakisLiaskos, M. (2023). The mechanism of Pseudomonas aeruginosa outer membrane vesicle biogenesis determines their protein composition. Proteomics, e2200464.
Zhang, H., Freitas, D., Kim, H. S., Fabijanic, K., Li, Z., Chen, H., Mark, M. T., Molina, H., Martin, A. B., Bojmar, L., Fang, J., Rampersaud, S., Hoshino, A., Matei, I., Kenific, C. M., Nakajima, M., Mutvei, A. P., Sansone, P., Buehring, W., … Lyden, D. (2018). Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation. Nature Cell Biology, 20, 332–343.
Zhang, H., Lu, J., Liu, J., Zhang, G., & Lu, A. (2020). Advances in the discovery of exosome inhibitors in cancer. Journal of Enzyme Inhibition and Medicinal Chemistry, 35, 1322–1330.
Zhang, J., Goodlett, D. R., Peskind, E. R., Quinn, J. F., Zhou, Y., Wang, Q., Pan, C., Yi, E., Eng, J., Aebersold, R. H., & Montine, T. J. (2005). Quantitative proteomic analysis of age-related changes in human cerebrospinal fluid. Neurobiology of Aging, 26, 207–227.
Zhang, K., Yue, Y., Wu, S., Liu, W., Shi, J., & Zhang, Z. (2019). Rapid capture and nondestructive release of extracellular vesicles using aptamer-based magnetic isolation. ACS Sensors, 4, 1245–1251.
Zhang, Q., Higginbotham, J. N., Jeppesen, D. K., Yang, Y. P., Li, W., McKinley, E. T., Graves-Deal, R., Ping, J., Britain, C. M., Dorsett, K. A., Hartman, C. L., Ford, D. A., Allen, R. M., Vickers, K. C., Liu, Q., Franklin, J. L., Bellis, S. L., & Coffey, R. J. (2019). Transfer of functional cargo in exomeres. Cell Reports, 27, 940–954.e6.
Zhang, Q., Jeppesen, D. K., Higginbotham, J. N., Graves-Deal, R., Trinh, V. Q., Ramirez, M. A., Sohn, Y., Neininger, A. C., Taneja, N., McKinley, E. T., Niitsu, H., Cao, Z., Evans, R., Glass, S. E., Ray, K. C., Fissell, W. H., Hill, S., Rose, K. L., Huh, W. J., … Coffey, R. J. (2021). Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets. Nature Cell Biology, 23, 1240–1254.
Zhang, S., Wear, D. J., & Lo, S. (2000). Mycoplasmal infections alter gene expression in cultured human prostatic and cervical epithelial cells. Fems Immunology and Medical Microbiology, 27, 43–50.
Zhang, X., Baht, G. S., Huang, R., Chen, Y. H., Molitoris, K. H., Miller, S. E., & Kraus, V. B. (2022). Rejuvenation of neutrophils and their extracellular vesicles is associated with enhanced aged fracture healing. Aging Cell, 21, e13651.
Zhang, X., Borg, E. G. F., Liaci, A. M., Vos, H. R., & Stoorvogel, W. (2020). A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity. Journal of Extracellular Vesicles, 9, 1791450.
Zhao, K., Bleackley, M., Chisanga, D., Gangoda, L., Fonseka, P., Liem, M., Kalra, H., Al Saffar, H., Keerthikumar, S., Ang, C. S., Adda, C. G., Jiang, L., Yap, K., Poon, I. K., Lock, P., Bulone, V., Anderson, M., & Mathivanan, S. (2019). Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling. Communications Biology, 2, 305.
Zhu, S., Ma, L., Wang, S., Chen, C., Zhang, W., Yang, L., Hang, W., Nolan, J. P., Wu, L., & Yan, X. (2014). Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS Nano, 8, 10998–11006.
Zomer, A., Maynard, C., Verweij, F. J., Kamermans, A., Schäfer, R., Beerling, E., Schiffelers, R. M., de Wit, E., Berenguer, J., Ellenbroek, S. I. J., Wurdinger, T., Pegtel, D. M., & van Rheenen, J. (2015). In vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior. Cell, 161, 1046–1057.
Zomer, A., Steenbeek, S. C., Maynard, C., & van Rheenen, J. (2016). Studying extracellular vesicle transfer by a Cre-loxP method. Nature Protocols, 11, 87–101.
Zong, S., Zong, J., Chen, C., Jiang, X., Zhang, Y., Wang, Z., & Cui, Y. (2018). Single molecule localization imaging of exosomes using blinking silicon quantum dots. Nanotechnology, 29, 065705.
Zonneveld, M. I., Brisson, A. R., van Herwijnen, M. J., Tan, S., van de Lest, C. H., Redegeld, F. A., Garssen, J., Wauben, M. H., & Nolte-’t Hoen, E. N. (2014). Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures. Journal of Extracellular Vesicles, 3, https://doi.org/10.3402/jev.v3.24215
