The relative importance of Escherichia coli exonuclease III and endonuclease IV for the hydrolysis of 3'-phosphoglycolate ends in polydeoxynucleotides.
Bacterial Proteins; Escherichia coli Proteins; Polydeoxyribonucleotides; Endodeoxyribonucleases; Exodeoxyribonucleases; exodeoxyribonuclease III; Deoxyribonuclease IV (Phage T4-Induced); endonuclease IV, E coli; DNA-(Apurinic or Apyrimidinic Site) Lyase; Magnesium; Bacterial Proteins/metabolism; Endodeoxyribonucleases/metabolism; Escherichia coli/enzymology; Exodeoxyribonucleases/metabolism; Magnesium/metabolism; Polydeoxyribonucleotides/metabolism; Genetics
Abstract :
[en] In vitro, in the presence of Mg++, the 3'-phosphoglycolatase activity of endonuclease IV is about 4-times smaller than that of exonuclease III for the same AP endonuclease activity. It thus seems that endonuclease IV has only a minor role in the repair of strand breaks limited by 3'-phosphoglycolate ends in Escherichia coli even after the amount of enzyme has been increased by induction with O2 -generating agents.
Disciplines :
Human health sciences: Multidisciplinary, general & others
Author, co-author :
Siwek, Brigitte; Biochimie, Faculté des Sciences, Université de Liège, Belgium
Bricteux-Grégoire, Suzanne; Université de Liège, 4000 Liège I, Belgium
Verly, Walter ; Relations académiques et scientifiques (Sciences)
Language :
English
Title :
The relative importance of Escherichia coli exonuclease III and endonuclease IV for the hydrolysis of 3'-phosphoglycolate ends in polydeoxynucleotides.
Alternative titles :
[fr] L'importance relative de l'exonucléase III et de l'endonucléase IV d'Escherichia coli pour l'hydrolyse des extrémités 3'-phosphoglycolate dans les polydéoxynucléotides.
Acknowledgments : This work was supported by grants from the Fonds Cancerolo-gique de la Caisse Generale d'Epargne et de Retraite and the Fonds de la Recherche Scientifique Medicale. V.B. was an Aspirant of the Fonds National de la Recherche Scientifique.
