Excision of apurinic sites from DNA with enzymes isolated from rat-liver chromatin.

Chromatin; DNA, Viral; Apurinic Acid; DNA; Endodeoxyribonucleases; Deoxyribonuclease IV (Phage T4-Induced); DNA-(Apurinic or Apyrimidinic Site) Lyase; Animals; Apurinic Acid/metabolism; Bacteriophage phi X 174/chemistry; Binding Sites; Chromatin/enzymology; DNA/isolation & purification; DNA, Viral/isolation & purification; Endodeoxyribonucleases/isolation & purification; Liver/enzymology; Rats; Biochemistry

Abstract :

[en] Apurinic sites were excised from phi X174 RF DNA with two enzymes isolated from rat liver chromatin: an apurinic/apyrimidinic endodeoxyribonuclease and a 5'-3'-exonuclease; the resulting gap was filled with DNA polymerase beta also prepared from rat liver chromatin and the repair was fully terminated with T4 ligase.

Disciplines :

Human health sciences: Multidisciplinary, general & others

Author, co-author :

Goffin, Colette ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'Ingénierie des Protéines (CIP)

Verly, Walter ; Relations académiques et scientifiques (Sciences)

Language :

English

Title :

Excision of apurinic sites from DNA with enzymes isolated from rat-liver chromatin.

Alternative titles :

[fr] Excision de sites apuriniques de l'ADN par des enzymes isolées de la chromatine du foie de rat.

Publication date :

October 1982

Journal title :

European Journal of Biochemistry

ISSN :

0014-2956

eISSN :

1432-1033

Publisher :

Wiley, England

Volume :

127

Issue :

Pages :

619 - 623

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1982.tb06917.x

Available on ORBi :

since 19 March 2024

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Bibliography

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