T4 DNA ligase can seal a nick in double-stranded DNA limited by a 5'-phosphorylated base-free deoxyribose residue.

DNA, Viral; Deoxyribose; Endodeoxyribonucleases; Deoxyribonuclease I; DNA Ligases; Polynucleotide Ligases; Animals; DNA Ligases/metabolism; Endodeoxyribonucleases/metabolism; Pancreas/enzymology; Phosphorylation; Polynucleotide Ligases/metabolism; Substrate Specificity; T-Phages/enzymology; Genetics

Abstract :

[en] The 5' AP endodeoxyribonucleases hydrolyze the phosphodiester bond 5' to AP (apurinic or apyrimidinic) sites in double-stranded DNA leaving 3'-OH and 5'-phosphate ends. These nicks are sealed by T4 DNA ligase although the 5'-phosphate end belongs to a base-free deoxyribose.

Disciplines :

Human health sciences: Multidisciplinary, general & others

Author, co-author :

Goffin, Colette ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'Ingénierie des Protéines (CIP)

Verly, Walter ; Relations académiques et scientifiques (Sciences)

Language :

English

Title :

T4 DNA ligase can seal a nick in double-stranded DNA limited by a 5'-phosphorylated base-free deoxyribose residue.

Alternative titles :

[fr] La T4 ADN ligase peut sceller une encoche dans l'ADN double brin limitée par un résidu de désoxyribose sans base 5'-phosphorylée.

Publication date :

25 November 1983

Journal title :

Nucleic Acids Research

ISSN :

0305-1048

eISSN :

1362-4962

Publisher :

Oxford University Press (OUP), England

Volume :

Issue :

Pages :

8103 - 8109

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://academic.oup.com/nar/article-pdf/11/22/8103/7048760/11-22-8103.pdf

Funding text :

Acknowledgments : This work was supported by grants from the Fonds de la Recherche Scientifique Mgdicale and the Fonds Cancfi- rologique de la Caisse Generale d'Epargne et de Retraite. C.G. was the recipient of a fellowship from the Institut pour la Recherche Scientifique Industrielle et Agricole.

Available on ORBi :

since 19 March 2024

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Bibliography

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