[en] Two nucleases active on alkylated-depurinated DNA have been extracted from rat liver chromatin with 1 M KCl. The major enzyme was purified to near homogeneity; it has a molecular weight of 12 500 (although some dimerization might occur), needs Mg2+ or Mn2+ for activity. The endonuclease activity is specific for apurinic/apyrimidinic sites in DNA; the enzyme has no associated exonuclease activity.
Disciplines :
Human health sciences: Multidisciplinary, general & others
Author, co-author :
Thibodeau, Lise; Laboratoire de Biochimie, Faculté Des Sciences De, Université de Liége, Institut de Chimie, Université de Liège Au Sart-Tilman, Liège, B-4000, Belgium
Bricteux, Suzanne; Laboratoire de Biochimie, Faculté Des Sciences De, Université de Liége, Institut de Chimie, Université de Liège Au Sart-Tilman, Liège, B-4000, Belgium
Verly, Walter ; Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Purification and properties of the major apurinic/apyrimidinic endodeoxyribonuclease of rat-liver chromatin.
Alternative titles :
[fr] Purification et propriétés de la principale endodésoxyribonucléase apurinique/apyrimidinique de la chromatine du foie de rat.
Verly W.G., Paquette Y. Can. J. Biochem. 1972, 50:217-224.
Verly W.G., Rassart E. J. Biol. Chem. 1975, 250:8214-8219.
Gossard F., Verly W.G. Eur. J. Biochem. 1978, 82:321-332.
Paquettc Y., Crine P., Verly W.G. (1972) Properties of the Endonuclease for Depurinated DNA fromEscherichia coli. Canadian Journal of Biochemistry 50:1199-1209.
Weiss B. J. Biol. Chem. 1976, 251:1896-1901.
Ljungquist S., Lindahl T., Howard‐Flanders P. J. Bacteriol. 1976, 126:646-653.
Verly W.G., Gossard F., Crine P. Proc. Natl Acad. Sci. USA 1974, 71:2273-2275.
