[en] Abstract
The application of Genotyping-by-Sequencing (GBS) approaches is often restricted in wildlife monitoring and conservation genetics, as those fields often rely on noninvasively collected samples with low DNA content. Here we selected a subset of informative single-nucleotide polymorphisms (SNPs) from genome-wide data for lineage discrimination of a locally endangered Eurasian rodent, the hazel dormouse (Muscardinus avellanarius), and designed a microfluidic 96 SNP genotyping assay suitable for noninvasively collected samples. Analyses of 43 samples from different European countries confirmed successful discrimination of the Eastern and Western lineage and local substructure within those lineages, proving the suitability of the developed panel for identifying evolutionary significant units and conservation units. Application with 94 hair and scat samples collected in a recent monitoring study on the hazel dormouse in Southern Germany resulted in >99.5% amplification success showing the applicability of the new tool in genetic wildlife monitoring and conservation studies.
Disciplines :
Zoology
Author, co-author :
Beez, Tobias; Senckenberg Research Institute and Natural History Museum Frankfurt
Leyhausen, Johanna; Senckenberg Research Institute and Natural History Museum Frankfurt
Mueller, Sarah; LMU Munich
von Thaden, Alina; Senckenberg Research Institute and Natural History Museum Frankfurt
Bertolino, Sandro; University of Turin
Büchner, Sven; Senckenberg Museum of Natural History Görlitz
Fietz, Joanna; University Hohenheim
Lang, Johannes; Justus-Liebig-University
Michaux, Johan ; Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Verbeylen, Goedele; Natuurpunt Mechelen
Nowak, Carsten; Senckenberg Research Institute and Natural History Museum Frankfurt
Cocchiararo, Berardino; Senckenberg Research Institute and Natural History Museum Frankfurt
Language :
English
Title :
Development of a microfluidic SNP assay for lineage discrimination in the endangered hazel dormouse
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