Article (Scientific journals)
Differentiation of Bone Marrow Monocytes into Alveolar Macrophages-like Cells through Co-culture with Lung Epithelial Cells and Group 2 Innate Lymphoid Cells.
Loos, Pauline; Marichal, Thomas; Machiels, Bénédicte et al.
2023In Bio-protocol, 13 (18), p. 4818
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Keywords :
Alveolar Niche; Alveolar macrophages; Co-culture; Group 2 innate lymphoid cells; Monocytes; Monocytes differentiation and polarization; Monocytes differentiation; polarization; Neuroscience (all); Biochemistry, Genetics and Molecular Biology (all); Immunology and Microbiology (all); Plant Science; General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; General Neuroscience
Abstract :
[en] During life, the embryonic alveolar macrophage (AM) population undergoes successive waves of depletion and replenishment in response to infectious and inflammatory episodes. While resident AMs are traditionally described as from embryonic origin, their ontogeny following inflammation or infection is much more complex. Indeed, it appears that the contribution of monocytes (MOs) to the AM pool is variable and depends on the type of inflammation, its severity, and the signals released in the microenvironment of the pulmonary niche (peripheral imprinting) and/or in the bone marrow (central imprinting). Deciphering the cellular and molecular mechanisms regulating the differentiation of MOs into AMs remains an area of intense investigation, as this could potentially explain part of the inter-individual susceptibility to respiratory immunopathologies. Here, we detail a relevant ex vivo co-culture model to investigate how lung epithelial cells (ECs) and group 2 lung innate lymphoid cells (ILC2s) contribute to the differentiation of recruited MOs into AMs. Interestingly, the presence of lung ILC2s and ECs provides the necessary niche signals to ensure the differentiation of bone marrow MOs into AMs, thus establishing an accessible model to study the underlying mechanisms following different infection or inflammation processes. Key features • Ex vivo co-culture model of the alveolar niche. • Deciphering the particular niche signals underlying the differentiation of MO into AMs and their functional polarization.
Disciplines :
Immunology & infectious disease
Author, co-author :
Loos, Pauline  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Marichal, Thomas  ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology
Machiels, Bénédicte  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Gillet, Laurent  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Language :
English
Title :
Differentiation of Bone Marrow Monocytes into Alveolar Macrophages-like Cells through Co-culture with Lung Epithelial Cells and Group 2 Innate Lymphoid Cells.
Publication date :
20 September 2023
Journal title :
Bio-protocol
eISSN :
2331-8325
Publisher :
Bio-protocol LLC, United States
Volume :
13
Issue :
18
Pages :
e4818
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
The protocol was adapted from the previously published paper: Loos et al. (2023). This work was supported, in part, by University of Liège (VIRIMPRINT ARC to L.G.), by Fondation Léon Fredericq (grant to P.L.), by the Fonds de la Recherche Scientifique—Fonds National Belge de la Recherche Scientifique (F.R.S./FNRS, “credit de recherche” J007515F to L.G.; “projet de recherche” T.0195.16 to L.G.; research associate support for B.M.; and research fellow for P.L. and C.M.), by Institut MERIEUX starting grant (to L.G.), by EOS joint programme of F.R.S./FNRS Fonds wetenschapellijk onderzoek–Vlaanderen-FWO (EOS ID:30981113 and 40007527) (to L.G.); and by ERC Starting Grant (ERC-StG-2020 VIROME, ID:853608) (to B.M.).
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