HPLC/ESI-MS Characterization of Phenolic Compounds from Cnicus benedictus L. Roots: A Study of Antioxidant, Antibacterial, Anti-Inflammatory, and Anti-Alzheimer's Activity.
[en] The phenolic composition of Cnicus benedictus roots from four Algerian regions was investigated. Extractions were performed in both hydro-methanolic (30 : 70, v/v) and hydro-ethanolic (30 : 70, v/v) solvents. Their efficiency was determined in terms of the qualitative and quantitative composition in phenolic compounds by HPLC-LC/MS of the different extracts isolated from C. Benedictus roots. Cnicus benedictus roots extract have been characterized by high content of phenolic compounds, where the trans chalcone, 2,3-dihydro flavone, 3-hydroxy flavone and cinnamic acid constitute the major components, in addition to fourteen minor acidic compounds and flavonoids as rutin. The hydro-methanolic extract was the richest in phenolic compounds yield from C benedictus. On the other hand, hydro methanolic (30 : 70, v/v) and hydro ethanolic (30 : 70, v/v) extracts exhibited a high anti-inflammatory activity by in vitro 5-lipoxygenase inhibitory activity (IC50 : 6.05±94.16 μg/mL) as well as by in silico docking according two methods. Likewise, anti-Alzheimer activity of extracts was confirmed by this last technique taking into account the major compounds identified. Antibacterial tests revealed interesting results compared to amoxicillin for the different regions studied with a high content in trans chalcone and 3-hydroxy Flavone.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Rezig, Khalid; Ecole Normale Supérieure El Bachir El-Ibrahimi (E.N.S), Ecology and Animal Laboratory, Department of Biology, BP 92, Kouba-Algiers, Algeria
Benkaci-Ali, Farid; University of Sciences and Technology HouariBoumediene (U.S.T.H.B), Laboratory of Functional Organic Analysis, Department of Organic Chemistry, Faculty of Chemistry, El Alia, BP 32, Bab Ezzouar, 16111, Algiers, Algeria
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Laurent, Sophie; University of Mons, Unit of General, Organic and Biomedical Chemistry, NMR and Molecular, Imaging Laboratory, 20, Place du Parc, B-7000, Mons, Belgium
Umar, Haruna Isiyaku; Department of Biochemistry, Federal University of Technology, Akure, Nigeria
Alex, Omoboyowa Damilola; Biochemical Pharmacology and Computational Biology Unit, Department of Biochemistry, Adekunle Ajasin University, Akungba-Akoko, Ondo State, Nigeria
Tata, Samira; Ecole Normale Supérieure El Bachir El-Ibrahimi (E.N.S), Ecology and Animal Laboratory, Department of Biology, BP 92, Kouba-Algiers, Algeria
Language :
English
Title :
HPLC/ESI-MS Characterization of Phenolic Compounds from Cnicus benedictus L. Roots: A Study of Antioxidant, Antibacterial, Anti-Inflammatory, and Anti-Alzheimer's Activity.
This work was supported by Laboratory of Functional Organic Analysis, Department of Organic Chemistry, Faculty of Chemistry, USTHB. We would like to thank Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech and Unit of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Liège for their collaboration.
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