Postnatal development of electrophysiological and morphological properties in layer 2/3 and layer 5 pyramidal neurons in the mouse primary visual cortex.
[en] Eye-opening is a critical point for laminar maturation of pyramidal neurons (PNs) in primary visual cortex. Knowing both the intrinsic properties and morphology of PNs from the visual cortex during development is crucial to contextualize the integration of visual inputs at different age stages. Few studies have reported changes in intrinsic excitability in these neurons but were restricted to only one layer or one stage of cortical development. Here, we used in vitro whole-cell patch-clamp to investigate the developmental impact on electrophysiological properties of layer 2/3 and layer 5 PNs in mouse visual cortex. Additionally, we evaluated the morphological changes before and after eye-opening and compared these in adult mice. Overall, we found a decrease in intrinsic excitability in both layers after eye-opening which remained stable between juvenile and adult mice. The basal dendritic length increased in layer 5 neurons, whereas spine density increased in layer 2/3 neurons after eye-opening. These data show increased number of synapses after onset of sensory input paralleled with a reduced excitability, presumably as homeostatic mechanism. Altogether, we provide a database of the properties of PNs in mouse visual cortex by considering the layer- and time-specific changes of these neurons during sensory development.
Research center :
Systems Neurophysiology, Institute of Zoology, RWTH Aachen University
Ciganok-Hückels, Natalja; Systems Neurophysiology, Institute of Zoology, RWTH Aachen University, 52074 Aachen, Germany ; Research Training Group 2416 MultiSenses-MultiScales, RWTH Aachen University, 52074 Aachen, Germany
Jehasse, Kevin ; Université de Liège - ULiège > Département d'électricité, électronique et informatique (Institut Montefiore) > Systèmes et modélisation
Kricsfalussy-Hrabár, Lena; Systems Neurophysiology, Institute of Zoology, RWTH Aachen University, 52074 Aachen, Germany
Ritter, Mira; Systems Neurophysiology, Institute of Zoology, RWTH Aachen University, 52074 Aachen, Germany
Rüland, Thomas; Systems Neurophysiology, Institute of Zoology, RWTH Aachen University, 52074 Aachen, Germany ; Research Training Group 2416 MultiSenses-MultiScales, RWTH Aachen University, 52074 Aachen, Germany ; Institute for Biological Information Processing (IBI-1), Forschungszentrum Jülich, 52428 Jülich, Germany
Kampa, Björn M ; Systems Neurophysiology, Institute of Zoology, RWTH Aachen University, 52074 Aachen, Germany ; Research Training Group 2416 MultiSenses-MultiScales, RWTH Aachen University, 52074 Aachen, Germany ; JARA BRAIN, Institute of Neuroscience and Medicine (INM-10), Forschungszentrum Jülich, 52428 Jülich, Germany
Language :
English
Title :
Postnatal development of electrophysiological and morphological properties in layer 2/3 and layer 5 pyramidal neurons in the mouse primary visual cortex.
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