Article (Scientific journals)
Fungal lectin, XCL, is internalized via clathrin-dependent endocytosis and facilitates uptake of other molecules.
Francis, Frédéric; Marty-Detraves, Claire; Poincloux, Renaud et al.
2003In European Journal of Cell Biology, 82 (10), p. 515-22
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Keywords :
Animals; Antibiotics, Antifungal/pharmacology; Cell Line; Chlorpromazine/pharmacology; Clathrin/metabolism; Clathrin-Coated Vesicles/metabolism; Endocytosis/physiology; Endosomes/metabolism; Filipin/pharmacology; Fungi/drug effects/metabolism; Green Fluorescent Proteins; Humans; Lectins/genetics/metabolism; Luminescent Proteins; Lysosomes/metabolism; Potassium/metabolism; Protein Transport/physiology
Abstract :
[en] The lectin isolated from Xerocomus chrysenteron (XCL) displays a toxic activity towards insects. In order to assess its possible mode of action and to gather useful data for its potential use in insect-resistant transgenic plants, we investigated the effects of XCL at the cellular level. Immunofluorescence microscopy studies revealed that XCL is rapidly internalized into small endocytic vesicles that further coalesce in the perinuclear region. We show that XCL is endocytosed by the clathrin-dependent pathway, and is delivered to late endosome/lysosome compartments. The internalization of XCL seems to be general since it occurs in different cell types such as insect (SF9) or mammalian (NIH-3T3 and Hela) cell lines. In the presence of XCL, the uptake of GFP and BSA is greatly enhanced, demonstrating that XCL facilitates endocytosis. Thus, XCL could serve as a delivery agent to facilitate the endocytosis of proteins that do not enter the cell alone.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Francis, Frédéric  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Marty-Detraves, Claire
Poincloux, Renaud
Baricault, Laurent
Fournier, Didier
Paquereau, Laurent
Language :
English
Title :
Fungal lectin, XCL, is internalized via clathrin-dependent endocytosis and facilitates uptake of other molecules.
Publication date :
2003
Journal title :
European Journal of Cell Biology
ISSN :
0171-9335
eISSN :
1618-1298
Publisher :
Wissenschaftliche Verlagsgesellschaft, Stuttgart, Germany
Volume :
82
Issue :
10
Pages :
515-22
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 02 December 2009

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