Induction of photosynthesis under anoxic condition in Thalassiosira pseudonana and Euglena gracilis: interactions between fermentation and photosynthesis.

Gain, Gwenaëlle; Berne, Nicolas; Feller, Tom; Godaux, Damien; Cenci, Ugo; Cardol, Pierre

doi:10.3389/fpls.2023.1186926

Article (Scientific journals)

Induction of photosynthesis under anoxic condition in Thalassiosira pseudonana and Euglena gracilis: interactions between fermentation and photosynthesis.

Gain, Gwenaëlle; Berne, Nicolas; Feller, Tom et al.

2023 • In Frontiers in Plant Science, 14, p. 1186926

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/310723

DOI
10.3389/fpls.2023.1186926

PubMed
37560033

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Keywords :

Euglena gracilis; Thalassiosira pseudonana; cyclic electron flow (CEF); fermentation; hydrogenase; photosynthesis; Plant Science

Abstract :

[en] [en] INTRODUCTION: In their natural environment, microalgae can be transiently exposed to hypoxic or anoxic environments. Whereas fermentative pathways and their interactions with photosynthesis are relatively well characterized in the green alga model Chlamydomonas reinhardtii, little information is available in other groups of photosynthetic micro-eukaryotes. In C. reinhardtii cyclic electron flow (CEF) around photosystem (PS) I, and light-dependent oxygen-sensitive hydrogenase activity both contribute to restoring photosynthetic linear electron flow (LEF) in anoxic conditions. METHODS: Here we analyzed photosynthetic electron transfer after incubation in dark anoxic conditions (up to 24 h) in two secondary microalgae: the marine diatom Thalassiosira pseudonana and the excavate Euglena gracilis. RESULTS: Both species showed sustained abilities to prevent over-reduction of photosynthetic electron carriers and to restore LEF. A high and transient CEF around PSI was also observed specifically in anoxic conditions at light onset in both species. In contrast, at variance with C. reinhardtii, no sustained hydrogenase activity was detected in anoxic conditions in both species. DISCUSSION: Altogether our results suggest that another fermentative pathway might contribute, along with CEF around PSI, to restore photosynthetic activity in anoxic conditions in E. gracilis and T. pseudonana. We discuss the possible implication of the dissimilatory nitrate reduction to ammonium (DNRA) in T. pseudonana and the wax ester fermentation in E. gracilis.

Disciplines :

Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Gain, Gwenaëlle ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Berne, Nicolas ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Feller, Tom ; Université de Liège - ULiège > Integrative Biological Sciences (InBioS)

Godaux, Damien ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Cenci, Ugo; Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille, CNRS, UMR8576 - UGSF, Lille, France

Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Language :

English

Title :

Induction of photosynthesis under anoxic condition in Thalassiosira pseudonana and Euglena gracilis: interactions between fermentation and photosynthesis.

Publication date :

2023

Journal title :

Frontiers in Plant Science

eISSN :

1664-462X

Publisher :

Frontiers Media SA, Switzerland

Volume :

Pages :

1186926

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.frontiersin.org/articles/10.3389/fpls.2023.1186926/full

Funding text :

PC acknowledges financial support from the Belgian Fonds de la Recherche Scientifique F.R.S.-F.N.R.S. (PDR T.0032), the BELSPO BRAIN project B2/212/PI/PORTAL, and European Research Council (ERC, H2020-EU BEAL project 682580). PC is Senior Research Associate from Fonds de la Recherche Scientifique – FNRS. AcknowledgmentsPC acknowledges financial support from the Belgian Fonds de la Recherche Scientifique F.R.S.-F.N.R.S. (PDR T.0032), the BELSPO BRAIN project B2/212/PI/PORTAL, and European Research Council (ERC, H2020-EU BEAL project 682580). PC is Senior Research Associate from Fonds de la Recherche Scientifique – FNRS.

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