[en] Thoroughbred (TB) racehorses undergo rigorous conditioning programs to optimize their physical and mental capabilities through varied exercise sessions. While conventional investigations focus on limited hematological and biochemical parameters, this field study employed untargeted metabolomics to comprehensively assess metabolic responses triggered by exercise sessions routinely used in TB conditioning. Blood samples were collected pre- and post-exercise from ten racehorses, divided into two groups based on exercise intensity: high intensity (n = 6, gallop at ± 13.38 m/s, 1400 m) and moderate intensity (n =4, soft canter at ± 7.63 m/s, 2500 m). Intensity was evaluated through monitoring of the speed, heart rate, and lactatemia. Resting and 30 min post-exercise plasma samples were analyzed using ultraperformance liquid chromatography coupled with high-resolution mass spectrometry. Unsupervised principal component analysis revealed exercise-induced metabolome changes, with high-intensity exercise inducing greater alterations. Following high-intensity exercise, 54 metabolites related to amino acid, fatty acid, nucleic acid, and vitamin metabolism were altered versus 23 metabolites, primarily linked to fatty acid and amino acid metabolism, following moderate-intensity exercise. Metabolomics confirmed energy metabolism changes reported by traditional biochemistry studies and highlighted the involvement of lipid and amino acid metabolism during routine exercise and recovery, aspects that had previously been overlooked in TB racehorses.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Bonhomme, Maëlle ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Patarin, Florence ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Kruse, Caroline ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Physiologie neuro-musculaire, de l'effort - Médecine sportive des animaux
François, Anne-Christine ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Renaud, Benoît ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Couroucé, Anne; Equine Department, Oniris, National Vet School of Nantes, 101 Route de Gachet, 44300 Nantes, France ; UR 7450 Biotargen, University of Caen Normandie, 3 Rue Nelson Mandela, 14280 Saint-Contest, France
Leleu, Claire; Equi-Test, La Lande, 53290 Grez-en-Bouère, France
Boemer, François ; Centre Hospitalier Universitaire de Liège - CHU > > Service de génétique
Toquet, Marie-Pierre; UR 7450 Biotargen, University of Caen Normandie, 3 Rue Nelson Mandela, 14280 Saint-Contest, France ; LABÉO (Frank Duncombe), 1 Route de Rosel, 14280 Saint-Contest, France
Richard, Eric A.; UR 7450 Biotargen, University of Caen Normandie, 3 Rue Nelson Mandela, 14280 Saint-Contest, France ; LABÉO (Frank Duncombe), 1 Route de Rosel, 14280 Saint-Contest, France
Seignot, Jérôme; Clinique Vétérinaire du Parc, 1 Avenue Malesherbes, 78600 Maisons-Laffitte, France
Wouters, Clovis ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Votion, Dominique ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
ULiège FSR - Université de Liège. Fonds spécial pour la recherche F.R.S.-FNRS - Fonds de la Recherche Scientifique IFCE - Institut Français du Cheval et de l'Équitation
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